QUANTITATIVE IMAGING: MORPHOMETRY ANALYSIS OF RHESUS FETAL LUNG

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 该项目的总体目标是将现代体视学方法引入ONPRC的定量成像项目。使用显微镜来获得定量信息而不是描述性信息，依赖于对三维几何、统计学和抽样理论概念的理解和实施，这些概念在生物学应用中并不常用。我们正在与中华人民共和国国际体视学学会副会长达拉斯·海德博士合作，为非人类灵长类动物肺形态计量的定量分析制定标准化方案。基于海德博士在成人肺的标准测量方面的经验，我们将为ONPRC目前正在进行的项目开发处理、采样、成像和分析恒河猴胎肺的方案。这个项目的总体目标有两个：1)设计肺的处理、分割和采样方案，以便为多名研究人员提供完整的器官全貌，可用于多种方法。这将有两个好处：它将提高器官使用的效率，并将确保每项研究都有足够的整个器官的代表性，以及2)开发、测试、实施并向ONPRC感兴趣的研究人员提供测量胎儿肺中感兴趣的各种结构参数的方案。体视学方法，有时枯燥乏味，在数学上令人望而生畏，将以易于遵循、简单的方案呈现。这种最初在肺部获得的经验将扩展到其他感兴趣的器官，从胰腺、卵巢和大脑区域开始。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general goal of this project is to introduce modern stereology methods to quantitative imaging projects at ONPRC. Using microscopy to obtain quantitative rather than descriptive information relies on the understanding and implementation of 3D geometry, statistics and sampling theory concepts, not commonly used in biological applications. We are collaborating with Dr. Dallas Hyde from the CNPRC, vice-president of the International Society for Stereology, to standardize protocols for the quantitative analysis of nonhuman primate lung morphometry. Based on Dr. Hyde's experience with standard measurements on adult lungs, we will develop protocols for processing, sampling, imaging and analyzing rhesus fetal lungs for projects currently in work at ONPRC. There are two general aims to this project: 1) to design protocols for processing, dividing and sampling the lung so that it can provide several investigators with complete representation of the whole organ, to be used for several methods. This will have a two-fold advantage: it will increase the efficiency or organ use and would ensure that each study has an adequate representation of the whole organ, and 2) to develop, test, implement and make available to interested ONPRC investigators protocols for measuring various structural parameters of interest in fetal lung. Stereology methods, sometimes tedious and mathematically intimidating will be presented as easy to follow, simple protocols. This initial experience obtained in lung will be then expanded to other organs of interest, starting with pancreas, ovaries and brain regions.