QUANTITATIVE IMAGING: MORPHOMETRY ANALYSIS OF RHESUS FETAL LUNG

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 该项目的总体目标是将现代体视学方法引入ONPRC的定量成像项目。 使用显微镜来获得定量而不是描述性信息依赖于对3D几何、统计和采样理论概念的理解和实施，这些概念在生物学应用中不常用。 我们正在与CNPRC的达拉斯海德博士（国际体视学学会副主席）合作，以标准化非人灵长类动物肺形态计量学定量分析的方案。 根据Hyde博士在成人肺标准测量方面的经验，我们将为ONPRC目前正在进行的项目开发处理、采样、成像和分析恒河猴胎肺的方案。 该项目有两个总体目标：1）设计用于处理、分割和采样肺的协议，以便它可以为几个研究者提供整个器官的完整表示，用于几种方法。 这将具有双重优势：它将增加器官使用的效率，并将确保每个研究都具有整个器官的充分代表性，以及2）开发，测试，实施并向感兴趣的ONPRC研究者提供用于测量胎儿肺中感兴趣的各种结构参数的方案。 体视学方法，有时繁琐和数学恐吓将提出易于遵循，简单的协议。 在肺部获得的初步经验将扩展到其他感兴趣的器官，从胰腺，卵巢和大脑区域开始。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general goal of this project is to introduce modern stereology methods to quantitative imaging projects at ONPRC. Using microscopy to obtain quantitative rather than descriptive information relies on the understanding and implementation of 3D geometry, statistics and sampling theory concepts, not commonly used in biological applications. We are collaborating with Dr. Dallas Hyde from the CNPRC, vice-president of the International Society for Stereology, to standardize protocols for the quantitative analysis of nonhuman primate lung morphometry. Based on Dr. Hyde's experience with standard measurements on adult lungs, we will develop protocols for processing, sampling, imaging and analyzing rhesus fetal lungs for projects currently in work at ONPRC. There are two general aims to this project: 1) to design protocols for processing, dividing and sampling the lung so that it can provide several investigators with complete representation of the whole organ, to be used for several methods. This will have a two-fold advantage: it will increase the efficiency or organ use and would ensure that each study has an adequate representation of the whole organ, and 2) to develop, test, implement and make available to interested ONPRC investigators protocols for measuring various structural parameters of interest in fetal lung. Stereology methods, sometimes tedious and mathematically intimidating will be presented as easy to follow, simple protocols. This initial experience obtained in lung will be then expanded to other organs of interest, starting with pancreas, ovaries and brain regions.