Cooperative Human Tissue Network

合作人体组织网络

• 批准号: 7233397
• 负责人: CARL D MORRISON
• 金额: $ 86.64万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7233397
• 关键词: biomedical registry /referral center; cancer information system; cancer registry /resource; case history; computer system design /evaluation; cooperative study; histopathology; human tissue; nucleic acids; tissue resource /registry

The objective of this proposal is to continue to operate a highly productive, efficient tissue procurement service at The Ohio State University Medical Center as a part of the Cooperative Human Tissue Network (CHTN) of the National Cancer Institute (NCI). This division will manage procurement consortiums both in Central (OSU Hospitals, James Cancer Hospital and Research Institute, and Riverside Methodist Hospital) and Northern Ohio (Cleveland Clinic) for research investigators in the United States and Canada. As the Midwestern Division of the CHTN we will continue to provide human normal, diseased, benign, and malignant surgical and autopsy tissues. This includes solid tissue, blood/sera, ascites fluids and pheresis specimens. Remnant tissues obtained for the CHTN will be examined and selected grossly then further quality controlled microscopically with histological review by a surgical pathologist. Tissues will be procured by investigator-specified guidelines and shipped within 24 hours or in batches according to the investigator's needs. Typical methods of procurement include liquid nitrogen (LN2) snap frozen tissues, OCT medium frozen tissue in LN2 cooled isopentane, gauze-wrapped fresh tissues in cooled saline, fresh tissue in transport tissue culture mediums and supplements, and formalin or gluteraldehyde fixed tissues. Paraffin- embedded fixed tissues are also available. In this grant new alternative methods for processed tissue will include RNase-inhibited prepared tissue, extracted tumor DNA and RNA, and slides of tumor sets in paraffin-embedded tissue matrix arrays. Investigator applications will be prioritized according to the CHTN charter. This division will continue to work closely with the other CHTN divisions to network investigator requests nationally, to educate the research community on the availability of CHTN specimens, to actively participate in the CHTN agendas to improve services and develop CHTN-wide policy and protocols. OSU Medical Center is instituting a system-wide informed consent for patients that will allow the use of specimens for research with outcome data or specimens will be delinked for complete anonymity. Specimens will continue to be coded to ensure patient confidentiality. OSU CHTN employees consist of anatomic technologists fully trained and supervised by pathologists in techniques of gross tissue examination, office personnel who perform database entry, update investigator's requests, distribute pathology reports, report statistical data, and gather donor-related medical information. In addition, the OSU CHTN personnel include a pathologist in charge of the daily operations.

本提案的目的是继续在俄亥俄州州立大学医学中心运营高产、高效的组织采购服务，作为国家癌症研究所（NCI）合作人体组织网络（CHTN）的一部分。该部门将为美国和加拿大的研究人员管理中部（OSU医院、詹姆斯癌症医院和研究所、滨江卫理公会医院）和北方俄亥俄州（克利夫兰诊所）的采购联盟。作为CHTN的中西部分部，我们将继续提供人类正常，患病，良性和恶性手术和尸检组织。这包括实体组织、血液/血清、腹水和分离标本。将对CHTN获得的残留组织进行检查，并进行大体选择，然后由外科病理学家进行组织学审查，进一步进行显微镜质量控制。组织将按照制药商规定的指南采购，并在24小时内或根据研究者的需要分批运输。典型的采购方法包括液氮（LN 2）快速冷冻组织、液氮冷却异戊烷中的OCT培养基冷冻组织、冷却盐水中的纱布包裹的新鲜组织、运输组织培养基和补充剂中的新鲜组织以及福尔马林或戊二醛固定的组织。石蜡包埋的固定组织也是可用的.在这项资助中，用于处理组织的新的替代方法将包括RNA酶抑制制备的组织，提取的肿瘤DNA和RNA，以及石蜡包埋组织基质阵列中的肿瘤组的载玻片。研究者申请将根据CHTN章程进行优先排序。该部门将继续与CHTN其他部门密切合作，在全国范围内建立研究者请求网络，教育研究界CHTN标本的可用性，积极参与CHTN议程，以改善服务并制定CHTN范围内的政策和协议。俄勒冈州立大学医学中心正在为患者制定一项全系统的知情同意书，允许使用标本进行研究，并提供结果数据，或将标本与完全匿名脱钩。将继续对标本进行编码，以确保患者机密性。OSU CHTN的员工包括接受过大体组织检查技术全面培训并由病理学家监督的解剖技术人员、执行数据库录入、更新研究者要求、分发病理学报告、报告统计数据和收集供体相关医疗信息的办公室人员。此外，OSU CHTN人员包括一名负责日常业务的病理学家。