Core D: Pathology and BioImaging
核心 D:病理学和生物成像
基本信息
- 批准号:10362708
- 负责人:
- 金额:$ 45.45万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-03-03 至 2026-07-31
- 项目状态:未结题
- 来源:
- 关键词:AftercareAnimalsArchivesBiological AssayBioluminescenceBiopsyBlood VesselsCD3 AntigensCD8B1 geneCLIA certifiedCell CommunicationCell Culture TechniquesCellsClinicalClinical TrialsClonal ExpansionClonalityCollectionColorColorectalComprehensive Cancer CenterComputer AssistedComputersDNA sequencingDevelopmentDoctor of PhilosophyDrug or chemical Tissue DistributionEffector CellEnsureEpitope spreadingEvaluationExhibitsFDA approvedFlow CytometryFluorescence MicroscopyFluorescent in Situ HybridizationFreezingGenesGenomicsGrowthHumanImageImage AnalysisImage CytometryImmuneImmune Response GenesImmunofluorescence ImmunologicImmunohistochemistryImmunologicsImmunotherapyInfrastructureIonsKineticsLaboratoriesLasersMagnetic Resonance ImagingMeasuresMicrosatellite InstabilityMusMutationMyeloid-derived suppressor cellsNew YorkOpticsOutcomeOvarianPD-1 blockadePD-1/PD-L1PTGS2 genePathologyPatientsPatternPopulationProcessProgram EvaluationProtocols documentationRecording of previous eventsRegimenRegulatory T-LymphocyteReportingReproducibilityResearch PersonnelResource SharingRoleSamplingScanningServicesSiteSourceSpecimenSystemT cell infiltrationT cell responseT-LymphocyteT-cell diversityTechnologyTestingTissue BanksTissue ProcurementsTissuesTranscriptTumor TissueUniversitiesVaccinationVaccinesWorkbioimagingchemokinechemokine receptordensitydesignexperimental studyimmune checkpointimmunological interventionimmunological statusin vivoin vivo imaginginnovationlymphoid structuresmedical schoolsmelanomamorphometrymouse modelmultimodalitynovelpathology imagingpreclinical studyprogrammed cell death ligand 1programmed cell death protein 1programsprotein expressionsample collectionsoundtissue culturetranscriptome sequencingtranscriptomicstreatment effecttumortumor growthtumor microenvironment
项目摘要
ABSTRACT
Core D (Pathology and Bio-Imaging Core), directed by Dr. Morrison, MD, will support this clinically oriented
Program by providing comprehensive state-of-the-art evaluation of the immune status of tumor
microenvironments (TME), using cutting-edge platforms for comprehensive immune profiling, cell and tissue
analysis, imaging and image-analysis. Core D includes the following Aims:
Aim 1: Provide infrastructure to collect, process, annotate and archive tumor samples and evaluate the
immune status of the TME using comprehensive immune profiling. Our Tissue Procurement Service will
help collect fresh, frozen and FFPE tumor specimens from the clinical trials of this Program and tissue banking
protocols. OmniSeq and multiplex IHC subcore, will perform immunologic, genomic, and transcriptomic
assessment of the immune status of the TME, using CLIA-certified Immune Report Card™ (IRC), to determine:
1) transcript levels of 384 immune genes, 2) mutational burden of each tumor, 3) microsatellite instability, and
4) copy number gain of PD-L1 and PD-L2. We will also evaluate 5) on-treatment changes in TCR beta
repertoire, to evaluate evidence of tumor-specific T cell response in the TME and 6) Patterns of expression of
PD-1/PD-L1/PD-L2 system and COX2 system in relation to T cell infiltrate and clinical outcomes.
Aim 2: Provide state-of-the-art immuno-fluorescence imaging and flow cytometry services to interrogate
the spatial effects of chemokine modulating agents on immune cell influx, effector- and immuno-suppressive
mechanisms in the TME. It will provide state-of-the-art multicolor flow and imaging cytometry, confocal laser
scanning and multicolor fluorescence microscopy, combined with computer morphometry and image analysis.
Aim 3 (Sub-Core D3): Perform small animal live imaging to determine the kinetics of tumor growth
(bioluminescence) in differentially-treated mice and the kinetics of CTL traffic and accumulation in TME.
Programmatic Role and Interactions. With Projects 1, 2 and 3 we will evaluate immune profiles of human
colorectal, ovarian and melanoma tumors in the course of patient treatment with chemokine-modulating
regimens and/or DC vaccines; determine changes in density, clonality and distribution of CTLs and other
immune cells in human and mouse tumors treated by CKM in the absence and presence of vaccination and
PD-1 blockade, and determine growth kinetics of the differentially treated and non-treated tumors in murine
models. We will also determine the impact of treatments on vascular normalization; and formation of tumor-
associated lymphoid structures implicated in epitope spreading and long term-treatment effects. Core D will
interact with Core A for prioritization of services, with Core B to assure that proper design of studies and sound
statistical evaluation of the results. We will work closely with Core C to ensure failsafe procurement of all
specimens from the clinical trials, samples annotation and evaluation in context of clinical outcomes.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
CARL D MORRISON其他文献
CARL D MORRISON的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('CARL D MORRISON', 18)}}的其他基金
相似海外基金
The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
- 批准号:
EP/Z000920/1 - 财政年份:2025
- 资助金额:
$ 45.45万 - 项目类别:
Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
- 批准号:
FT230100276 - 财政年份:2024
- 资助金额:
$ 45.45万 - 项目类别:
ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
- 批准号:
MR/X024261/1 - 财政年份:2024
- 资助金额:
$ 45.45万 - 项目类别:
Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
- 批准号:
DE240100388 - 财政年份:2024
- 资助金额:
$ 45.45万 - 项目类别:
Discovery Early Career Researcher Award
Zootropolis: Multi-species archaeological, ecological and historical approaches to animals in Medieval urban Scotland
Zootropolis:苏格兰中世纪城市动物的多物种考古、生态和历史方法
- 批准号:
2889694 - 财政年份:2023
- 资助金额:
$ 45.45万 - 项目类别:
Studentship
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
- 批准号:
2842926 - 财政年份:2023
- 资助金额:
$ 45.45万 - 项目类别:
Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
- 批准号:
NC/X001644/1 - 财政年份:2023
- 资助金额:
$ 45.45万 - 项目类别:
Training Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
- 批准号:
2337595 - 财政年份:2023
- 资助金额:
$ 45.45万 - 项目类别:
Continuing Grant
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
- 批准号:
2232190 - 财政年份:2023
- 资助金额:
$ 45.45万 - 项目类别:
Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
- 批准号:
23K17514 - 财政年份:2023
- 资助金额:
$ 45.45万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)














{{item.name}}会员




