Biospecimen and Pathology Core

生物样本和病理学核心

• 批准号: 10473658
• 负责人: CARL D MORRISON
• 金额: $ 9.86万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-18 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10473658
• 关键词: American; American Society of Clinical Oncology; Archives; Biological Assay; Blood specimen; Clinical; Clinical Research; Clinical Trials; Collection; Coupled; Cytometry; DNA methylation profiling; Data; Diagnostic; Drug or chemical Tissue Distribution; Eligibility Determination; Evaluation; Experimental Designs; Guidelines; Histologic; Histology; Histopathology; Human; Human Resources; Image; Immune; Immunogenomics; Immunohistochemistry; Immunologics; Individual; Informatics; Infrastructure; Ischemia; Malignant neoplasm of ovary; Pathologic; Pathologist; Pathology; Patients; Performance; Phenotype; Preparation; Process; Protocols documentation; Quality Control; Research Personnel; Research Project Grants; Research Support; Sampling; Scanning; Services; Site; Slide; Specimen; Standardization; T cell receptor repertoire sequencing; Testing; Time; Tissue Microarray; Tissues; Training; Translational Research; Tumor Tissue; Tumor-Infiltrating Lymphocytes; automated image analysis; base; biobank; college; data dissemination; digital; digital imaging; exome sequencing; experience; immunological status; informatics infrastructure; interest; laser capture microdissection; next generation sequencing; ovarian neoplasm; sample fixation; tissue resource; tool; transcriptome sequencing; tumor; tumor microenvironment

ABSTRACT The Roswell Park Ovarian Cancer SPORE Biospecimen & Pathology Core (BPC) combines a centralized mechanism for expert tissue handling services and performance of tissue-based assays by highly qualified, experienced personnel with a state-of-the-art integrated informatics infrastructure which provides tools for tracking biospecimen collection, histopathological evaluation, and clinical annotation of samples with linkage to immunogenomic and other translational data generated by the Individual Research Projects proposed in this renewal application. The Core will collect, de-identify, process, bank, and distribute annotated, high-quality tumor and blood samples from patients participating in SPORE-sponsored clinical studies. In addition, the BPC will provide investigators with specialized technical and interpretative histopathology services including expertise in multiplexed immunohistochemistry (IHC) and imaging mass cytometry (IMC), tissue microarray construction, laser capture microdissection, digital imaging, and quantitative pathology review of slides, tumor- infiltrating lymphocytes assessment, tissue QC/QA and preparation for next-gen sequencing-based assays (whole exome seq, RNAseq, TCRseq, MethylSEQ), and oversight on optimal utilization of each biospecimen. The Core will also be instrumental in testing patient biospecimens to determine eligibility for clinical trials and directly add to the bi-directional translational science by a detailed immunological annotation of ovarian tumors.

抽象的 罗斯威尔公园卵巢癌孢子生物镜和病理学核心（BPC）结合了集中式 专家组织处理服务的机制以及高素质的基于组织测定的性能 经验丰富的人员具有最先进的综合信息学基础架构，可为 跟踪生物传播收集，组织病理学评估以及与与 在此提出的个人研究项目产生的免疫基因组和其他翻译数据 更新申请。核心将收集，取消识别，处理，银行和分发带注释的高质量 参加孢子赞助的临床研究的患者的肿瘤和血液样本。此外，BPC 将为研究人员提供专业的技术和解释性组织病理学服务，包括 多重免疫组织化学（IHC）和成像质量细胞术（IMC），组织微阵列的专业知识 构建，激光捕获微解剖，数字成像和幻灯片，肿瘤的定量病理学评论 浸润淋巴细胞评估，组织QC/QA和基于下一代测序的测定 （整个外显子Seq，rnaseq，tcrseq，甲基解释），以及对每个生物测量最佳利用的监督。 该核心还将有助于测试患者生物测量以确定临床试验的资格和 通过对卵巢肿瘤的详细免疫学注释直接添加到双向转化科学。