Molecular mechanisms of queen bee longevity

蜂王长寿的分子机制

• 批准号: 7114879
• 负责人: GENE E ROBINSON
• 金额: $ 29.88万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7114879
• 关键词: Hymenoptera; adenosine triphosphate; aging; animal colony; animal population genetics; animal population study; antioxidants; biological models; biological signal transduction; cellular respiration; electron transport; ethology; free radical oxygen; gene expression; gene induction /repression; genetically modified animals; longevity; microarray technology; mitochondria; model design /development; oxidative stress; polymerase chain reaction

DESCRIPTION (provided by applicant): We propose to use the honey bee as a model to determine which of the mechanisms that can be manipulated to increase lifespan actually have been in nature. The queen bee is highly reproductively active but typically lives 10-fold longer than does the worker bee. Using the free radical damage theory of aging as a foundation, we will: 1) Determine whether queen-worker longevity differences are associated with differences in expression of genes encoding antioxidants, electron chain proteins, or both. We have complete or near complete sequence for 25 genes related to longevity: 15 antioxidant, 8 mitochondrial, and 2 signal transduction genes; 12 will be analyzed with real-time quantitative RT-PCR and the rest are on a Cdna microarray (below). Analyses will be supplemented with protein measures in selected cases. Preliminary results indicate striking differences in gene expression, especially early in life. Measurements of ATP production and effects of ROS damage will test the functional significance of the differences; 2) Determine the causal basis of some of the queen-worker differences in gene expression with transgenic flies. Three of the genes with some of the strongest queen-worker differences will be selected and transgenic flies made (up- and downregulation) in the laboratory of collaborator J. Tower. Collaborator K. Hughes will study their age-specific survival and reproduction, and developmental rate. Hughes will also work with long- and-short lived selected lines of flies to determine whether selection acted on some of the same genes that differ in queens vs. workers; and 3) Conduct a microarray survey to identify additional genes that differ in expression between queens and workers, to determine what related pathways are affected in association with differences in the specific genes studied in Aim 1. We will use our recently developed cDNA microarrays which represent ca. 6000 different bee genes, including additional antioxidant and respiration-related genes, and genes encoding different HSPs. The principal significance of this research is that it will identify naturally occurring molecular mechanisms promoting longevity. The value of the bee as an aging model will be enhanced significantly with the expected completion of genome sequencing later in '03.

描述（由申请人提供）：我们建议使用蜜蜂作为模型来确定自然界中实际上存在哪些可被操纵以延长寿命的机制。蜂王的繁殖能力很强，但寿命通常比工蜂长 10 倍。以衰老的自由基损伤理论为基础，我们将：1）确定蚁后与工蚁的寿命差异是否与编码抗氧化剂、电子链蛋白或两者的基因表达差异有关。我们拥有25个与长寿相关的基因的完整或接近完整的序列：15个抗氧化剂基因、8个线粒体基因和2个信号转导基因； 12 个将使用实时定量 RT-PCR 进行分析，其余的则在 Cdna 微阵列上进行分析（如下）。在某些情况下，分析将辅以蛋白质测量。初步结果表明基因表达存在显着差异，尤其是在生命早期。 ATP 产生和 ROS 损伤影响的测量将测试差异的功能意义； 2) 确定转基因果蝇的一些蜂王-工蜂基因表达差异的因果基础。将选择具有最强蚁后-工蚁差异的三个基因，并在合作者 J. Tower 的实验室中制作转基因果蝇（上调和下调）。合作者 K. Hughes 将研究它们特定年龄的生存和繁殖以及发育速度。休斯还将研究长寿命和短寿命的选定果蝇品系，以确定选择是否作用于一些相同的基因，而这些基因在蚁后和工蚁中有所不同； 3) 进行微阵列调查，以确定蚁后和工蜂之间表达差异的其他基因，以确定与目标 1 中研究的特定基因的差异相关的哪些相关途径受到影响。 6000 个不同的蜜蜂基因，包括额外的抗氧化和呼吸相关基因，以及编码不同 HSP 的基因。这项研究的主要意义在于，它将确定自然发生的促进长寿的分子机制。随着基因组测序预计在 03 年晚些时候完成，蜜蜂作为衰老模型的价值将得到显着增强。

项目成果

Senescence in the worker honey bee Apis Mellifera.

• DOI: 10.1016/j.jinsphys.2007.05.015
• 发表时间: 2007-10
• 期刊: Journal of insect physiology
• 影响因子: 2.2
• 作者: Silvia C. Remolina;Daniel M Hafez;G. Robinson;K. Hughes

Vitellogenin family gene expression does not increase Drosophila lifespan or fecundity.

• DOI: 10.12688/f1000research.3975.1
• 发表时间: 2014
• 期刊: F1000Research
• 作者: Ren Y;Hughes KA
• 通讯作者: Hughes KA

Age specificity of inbreeding load in Drosophila melanogaster and implications for the evolution of late-life mortality plateaus.

果蝇近交负荷的年龄特异性及其对晚年死亡率高原演变的影响。

• DOI: 10.1534/genetics.106.070078
• 发表时间: 2007
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Reynolds,RoseM;Temiyasathit,Sara;Reedy,MelissaM;Ruedi,ElizabethA;Drnevich,JennyM;Leips,Jeff;Hughes,KimberlyA
• 通讯作者: Hughes,KimberlyA

Genes of the antioxidant system of the honey bee: annotation and phylogeny.

• DOI: 10.1111/j.1365-2583.2006.00695.x
• 发表时间: 2006-10
• 期刊: Insect molecular biology
• 影响因子: 2.6
• 作者: Corona M;Robinson GE
• 通讯作者: Robinson GE