Instrumental Designs for Near Infrared Glucose Monitors

描述(申请人提供)：描述了两种用于非侵入性血糖测量的新型固态仪器设计。这两种仪器都实现了近红外光通过组织样本的透射率测量，并允许在硬件中对多变量校准模型进行编码。每台仪器都基于以滤波器带通函数的形式指定的预定义的近红外波长组合的顺序测量。在这两种设计中，使用数字微镜阵列(MMA)或声光可调谐滤光器(AOT F)来产生这些滤光器轮廓。MMA允许指定任意复杂性的滤波器轮廓，而AOTF允许生成具有更简单方波形状的滤波器。描述了一种计算模型和数值优化过程，用于定义导致测量对血糖具有选择性的独特光谱模式的最佳滤光片。这些滤光片轮廓类似于由多变量校准模型从常规光谱仪获取的连续光谱数据中提取的分量。执行这些基于离散过滤器的测量可获得吞吐量和多路复用优势，并允许创建与糖尿病患者在实验室受控环境之外进行的非侵入性血糖测量兼容的坚固的仪器设计。体外实验和以大鼠模型进行的体内研究都被建议用于表征这些仪器设计的性能和分析实用性。实验和计算工作将针对开发非侵入性夜间低血糖报警器的总体目标。

DESCRIPTION (provided by applicant): Two novel solid-state instrumental designs are described for use in the noninvasive measurement of blood glucose. Both instruments implement transmission measurements of near-infrared light through a tissue sample and allow the encoding of multivariate calibration models in hardware. Each instrument is based on the sequential measurement of pre-defined combinations of near- infrared wavelengths that are specified in the form of filter bandpass functions. In the two designs, these filter profiles are generated with either a digital micromirror array (MMA) or an acousto-optic tunable filter (AOT F). The MMA allows the specification of filter profiles of arbitrary complexity, while the AOTF allows the generation of filters with simpler square-wave shapes. A computational model and numerical optimization procedures are described for use in defining optimal filter profiles that result in the measurement of unique spectral patterns that are selective for blood glucose. These filter profiles are analogous to the components extracted by multivariate calibration models from contiguous spectral data acquired by a conventional spectrometer. Performing these discrete filter-based measurements gains both throughput and multiplex advantages, and allows the creation of rugged instrumental designs that are compatible with noninvasive blood glucose measurements made by diabetic patients outside the controlled environment of the laboratory. Both in vitro experiments and in vivo studies with a rat model are proposed for use in characterizing the performance and analytical utility of these instrumental designs. The experimental and computational work will be directed to the overall goal of developing a noninvasive nocturnal hypoglycemic alarm.