Continuous near infrared glucose sensor

描述（由申请人提供）：一项研究计划，描述了开发一种新型的小型化固态光谱仪设计的分析测量的组合区域的近红外光谱（2.0-2.5？m或4000-5000 cm-1）。我们的目标是开发一个强大的光谱仪微传感器，能够在实验室外的非理想和不受控制的环境条件下进行准确的分析测量。迄今为止的进展证明了这种方法的可行性和实用性，连续测量的超滤探头植入实验室大鼠皮下收集的间质液中的葡萄糖。确定了当前光谱仪的改进，以从一分钟内收集的光谱中实现1 mM葡萄糖的检测限。该研究计划旨在通过提高定制半导体发光二极管（LED）的输出来实现这些改进，提高创新焦平面阵列探测器系统的探测能力，实施同伴参考通道，并开发数据分析方法以提高校准模型的长期稳定性。提出了一种新的电子接口来定制和简化数据采集。光谱仪原型将在一系列体外和体内实验中进行表征和验证，包括在非麻醉、自由活动的实验室大鼠中进行连续和选择性葡萄糖测量。在所要求的四年资助期结束时，我们的目标是拥有一个优化的近红外微传感器光谱仪，可以在人体试验中进行评估，用于连续测量葡萄糖。公共卫生相关性提出了一种新型葡萄糖传感器，该传感器基于一种创新的小型化全固态近红外光谱仪，该光谱仪由一组定制的光电器件构成。总体目标是在非实验室条件下在一分钟内测量临床相关的葡萄糖水平。将在实验室动物中验证分析特性。

DESCRIPTION (provided by applicant): A research program is described to develop a novel miniaturized solid-state spectrometer designed for analytical measurements over the combination region of the near infrared spectrum (2.0-2.5 ?m or 4000-5000 cm-1). The objective is to develop a robust spectrometer microsensor that is capable of accurate analytical measurements under non-ideal and uncontrolled ambient conditions outside the laboratory. Progress to date demonstrates the feasibility and utility of this approach for the continuous measurement of glucose in interstitial fluid collected by an ultrafiltration probe implanted subcutaneously in laboratory rats. Improvements in the current spectrometer are identified to achieve a limit of detection of 1 mM for glucose from spectra collected over a one-minute period. The research plan is designed to realize these improvements by enhancing the output of custom-made semiconductor light emitting diodes (LEDs), improving the detectivity of an innovative focal plan array detector system, implementing a companion reference channel, and developing data analysis methods to improve the long-term stability of the calibration model. A new electronic interface is proposed to customize and miniaturize data acquisition. Spectrometer prototypes will be characterized and validated in a series of in vitro and in vivo experiments that include continuous and selective glucose measurements in non-anesthetized, freely moving laboratory rats. At the end of the requested four-year grant period, our objective is to have an optimized near infrared microsensor spectrometer that is ready for evaluation in human trials for the continuous measurement of glucose. PUBLIC HEALTH RELEVANCE A novel glucose sensor is proposed based on an innovative miniaturized, all solid-state near infrared spectrometer that is constructed from a set of customized optoelectronic devices. The overall objective is to measure clinically relevant levels of glucose in one minute under non- laboratory conditions. Analytical properties will be validated in laboratory animals.