Otopathology by Light Microscopy and Molecular Techniques

光学显微镜和分子技术的耳病理学

• 批准号: 7186016
• 负责人: SAUMIL N. MERCHANT
• 金额: $ 61.83万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7186016
• 关键词: clinical research; human; light microscopy; proteomics; temporal bone

DESCRIPTION (provided by applicant): A knowledge of the pathologic basis of disease is central to the study of medicine, including disorders affecting the auditory and vestibular systems. Otology is unique in that the inner ear is inaccessible during life and so, conventional techniques of pathologic study such as biopsy and surgical excision are not feasible. Consequently, insight into the pathologic basis of ear disease can only be obtained by postmortem study of temporal bones and by developing credible animal models. An improved understanding of the pathology and pathogenesis of disorders affecting the auditory and vestibular systems should lead to more rational diagnosis and clinical management of these diseases. The procurement, processing and study of human temporal bones is a time-consuming and costly endeavor which lies outside the purview of clinical departments of pathology, and is therefore a research endeavor that is performed in temporal bone laboratories. This proposal was developed in keeping with the guidelines announced in the RFA (DC-06-001) that is meant to establish the Human Temporal Bone Consortium for Research Resource Enhancement. Our goals include the following: 1. To develop customized protocols for assessment of prospectively acquired temporal bones. 2. To archive in digital format the histopathology reports and images for all temporal bones in order to enhance the research and educational value of archival and newly acquired specimens. 3. To develop improved protocols for applying molecular biologic assays to temporal bone research, especially protocols that will optimize the applicability of immunostaining and mass spectrometry- based proteomics while preserving the morphology of the processed tissues. 4. To collect comprehensive clinical information from registered temporal bone donors. 5. To establish a program for the training of future temporal bone researchers. 6. To cooperate with the Temporal Bone Research Consortium. This research will provide new knowledge about the causes of disorders of hearing and balance, which should lead to better methods of diagnosis and therapy.

描述（由申请人提供）：了解疾病的病理基础是医学研究的核心，包括影响听觉和前庭系统的疾病。耳科的独特之处在于，内耳在一生中是无法接触到的，因此传统的病理研究技术（例如活检和手术切除）是不可行的。因此，只有通过对颞骨进行尸检并开发可靠的动物模型，才能深入了解耳部疾病的病理基础。对影响听觉和前庭系统的疾病的病理学和发病机制的进一步了解应该有助于对这些疾病进行更合理的诊断和临床治疗。人类颞骨的采集、处理和研究是一项耗时且昂贵的工作，超出了病理学临床部门的范围，因此是在颞骨实验室中进行的研究工作。该提案是根据 RFA (DC-06-001) 中宣布的指南制定的，该指南旨在建立人类颞骨联盟以增强研究资源。我们的目标包括： 1. 开发定制方案来评估预期获得的颞骨。 2. 以数字格式存档所有颞骨的组织病理学报告和图像，以提高档案和新获取标本的研究和教育价值。 3. 开发改进的方案，将分子生物学测定应用于颞骨研究，特别是优化免疫染色和基于质谱的蛋白质组学的适用性，同时保留处理组织的形态的方案。 4. 从登记的颞骨捐献者那里收集全面的临床信息。 5. 制定未来颞骨研究人员的培训计划。 6. 与颞骨研究联盟合作。这项研究将提供有关听力和平衡障碍原因的新知识，从而带来更好的诊断和治疗方法。