MECHANISTIC STUDIES OF ELECTRON CAPTURE DISSOCIATION BY DEUTERIUM LABELING

描述：MALDI质谱学已成为推动质谱学在生物化学中应用的两种重要方法之一。阻碍广泛应用的一个问题是一些分子表现出的广泛的亚稳态离子衰变。当一类分子具有一个或多个不稳定基团时，这是一个特别的问题，这些基团经历的反应的碎裂障碍比那些对分析有用的碎裂要低得多。一个例子是水的损失，关于这一点的信息内容很少。当遇到混合物时，问题会变得更糟。复合碎片即使不是不可能分配，也很难分配。克服这一限制的一个有希望的发展是在浴气存在的情况下启动解吸过程。这一想法将被用于分析含有低聚糖、糖肽和磷酸肽、唾液酸化糖脂、硫化低聚糖和含有氧化半胱氨酸残基的多肽的样品。波士顿大学研究资源的合作者对这些样本中的许多都感兴趣。

DESCRIPTION: MALDI mass spectrometry has become one of the two important methods driving the application of mass spectrometry in biochemistry. A problem that stands in the way of broad application is the extensive metastable-ion decay exhibited by some molecules. This is a particular problem when a class of molecules has one or more labile groups that undergo reactions with significantly lower fragmentation barriers than those for analytically useful fragmentations. An example is the loss of water, for which there is little information content. The problem is made worse when a mixture is encountered. The composite fragmentation is difficult, if not impossible, to assign. A promising development to overcome this limitation is to initiate the desorption process in the presence of a bath gas. This idea will be pursued for the analysis of samples containing oligosaccharides, glyco and phosphopeptides, sialylated glycolipids, sulfated oligosaccharides, and peptides containing oxidized cysteine residues. Many of these samples are of interest to collaborators of the BU research resource.