DIFFERENTIATION OF ASPARTIC VERSUS ISO-ASPARTIC ACID RESIDUES IN PEPTIDES

肽中天冬氨酸残基与异天冬氨酸残基的区分

• 批准号: 7182228
• 负责人: PETER B. O'CONNOR
• 金额: $ 4.04万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7182228
• 关键词: biomedical equipment resource; biomedical resource; mass spectrometry; method development

DESCRIPTION: MALDI mass spectrometry has become one of the two important methods driving the application of mass spectrometry in biochemistry. A problem that stands in the way of broad application is the extensive metastable-ion decay exhibited by some molecules. This is a particular problem when a class of molecules has one or more labile groups that undergo reactions with significantly lower fragmentation barriers than those for analytically useful fragmentations. An example is the loss of water, for which there is little information content. The problem is made worse when a mixture is encountered. The composite fragmentation is difficult, if not impossible, to assign. A promising development to overcome this limitation is to initiate the desorption process in the presence of a bath gas. This idea will be pursued for the analysis of samples containing oligosaccharides, glyco and phosphopeptides, sialylated glycolipids, sulfated oligosaccharides, and peptides containing oxidized cysteine residues. Many of these samples are of interest to collaborators of the BU research resource.

MALDI质谱已成为推动质谱在生物化学中应用的两种重要方法之一。 一个问题，站在方式的广泛应用是广泛的亚稳离子衰变所表现出的一些分子。 当一类分子具有一个或多个不稳定基团时，这是一个特别的问题，所述不稳定基团经历的反应具有比用于分析有用的碎片化的那些显著更低的碎片化屏障。 一个例子是水的流失，这方面的信息内容很少。 当遇到混合物时，问题变得更糟。 如果不是不可能的话，也很难分配复合碎片。 克服这种限制的一个有希望的发展是在浴气的存在下启动解吸过程。 这一想法将被追求的分析样品含有寡糖，糖和磷酸肽，唾液酸化糖脂，硫酸化寡糖，并含有氧化半胱氨酸残基的肽。 这些样本中的许多都是BU研究资源的合作者感兴趣的。