ATOMIC FORCE MICROSCOPE: MAGNETIC FORCE MICROSCOPY

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：戴维斯心肺研究所(DHLRI)正在申请一个原子力显微镜(AFM)系统，以在俄亥俄州立大学(OSU)医学和公共卫生学院(COMPH)建立AFM核心设施。所要求的仪器是数字仪器公司的生物显微镜AFM系统。该系统将由数字仪器的纳米IV控制器、生物显微镜AFM、用于数据分析的离线工作站和蔡司倒置光学显微镜Axiovert 200以及一些必要的AFM附件和图像处理软件组成。俄亥俄州立大学的COMPH和其他联合生物医学学院没有任何AFM仪器。我们目前的访问仅限于在相当远的俄亥俄州立大学西校区的工程学院升级的多模AFM和纳米ILIA控制器。除了物理上远离COMPH，这种原子力显微镜对样品大小和有限的技术能力有限制，只有通过像生物镜这样的仪器才能克服这些限制。生物显微镜对于容纳俄亥俄州立大学的数量和多样性的研究项目是必不可少的，这些项目将受益于AFM技术。这项关于购买最先进的AFM系统的提案是代表三个主要用户提交的，其中三个用户由NIH提供资金，四个小用户由NSF或其他资金提供资金。为了满足用户群体多样化的AFM需求，需要一种最先进的AFM仪器，既能完成高分辨率的纳米级成像，又能同时对细胞样本和组织切片进行光学显微镜检查。美国国立卫生研究院的项目将使用所要求的生物显微镜AFM来研究细胞骨架、细胞胞吐作用和细胞外基质调节、组织切片的形貌和表面特征。这些研究将有助于回答纳米级的生物学问题，然后这些问题将与NIH项目中使用多种其他技术获得的数据相关。AFM的新生物学应用，如磁力显微镜(MFM)和浸渍笔纳米光刻(DPN)，将形成拟议的AFM核心设施的创新研究和开发的新载体。研究所和大学为相关实验室设施的开发提供了支持，并将以工作人员工资的形式提供持续的机构支持。收购这种AFM仪器将极大地增强主要用户的研究计划，并帮助俄亥俄州立大学的其他生物医学研究人员。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The Davis Heart and Lung Research Institute (DHLRI) is requesting an atomic force microscope (AFM) system to establish an AFM core facility at the Ohio State University (OSU) College of Medicine and Public Health (COMPH). The requested instrument is a Bioscope AFM system from Digitial Instruments. The system will be comprised of a Digital Instrument's Nanoscope IV controller, a BioScope AFM, an offline workstation for data analysis and the Zeiss inverted light microscope Axiovert 200 along with a number of necessary AFM accessories and the image processing softwares. The COMPH and other allied Colleges of Biomedical Sciences at OSU do not have any AFM instrumentation. Our current access is limited to an upgraded MultiMode AFM with a Nanoscope Ilia controller in the College of Engineering, a considerable distance away at the West Campus of OSU. Besides being physically away from the COMPH, this AFM has restrictions on the sample size and limited technical capabilities which can only be overcome by an instrument like the Bioscope. The Bioscope is essential to accommodate the quantity and diversity of the research projects at OSU that will benefit by using the AFM technology. This proposal for acquiring a state-of-the art AFM system is being submitted on behalf of 3 major users with NIH funding and 4 minor users with NSF or other funding. To fulfill the diverse AFM needs of the user group, a state-of-the-art AFM instrument is needed which can accomplish both high-resolution nanoscale imaging and a simultaneous light-microscopy examination of the cell samples and tissue sections. The NIH projects will use the requested Bioscope AFM for investigating cell cytoskeleton, exocytosis and extracellular matrix regulation by cells, topography of tissue sections and surface characterization. These investigations will help in answering biological questions at the nanoscale level which will then be related to the data acquired using a multitude of other techniques in the NIH projects. Novel biological applications of AFM like magnetic force microscopy (MFM) and dip-pen nanolithography (DPN) will form a new vector of innovative research and development of the proposed AFM core facility. DHLRI and the University have provided support for the related laboratory facility development and will provide continuing institutional support in the form of staff salary. Acquisition of this AFM instrumentation will greatly enhance the research programs of the major users and aid other biomedical investigators at OSU.