ISOTOPE RATIO MASS SPECTROMETER: OBESITY: US BLACK & NIGERIAN WOMEN

同位素比质谱仪：肥胖：美国黑人

• 批准号: 7335007
• 负责人: DALE A SCHOELLER
• 金额: $ 3.67万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335007
• 关键词: fasting; obesity; university; water

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We request funds for the purchase of an isotope ratio mass spectrometer for 18O and 13C analysis. We have selected the Thermoelectron Delta Plus equipped with a Gas Bench II for automated 18O analysis in CO2and a Combustion Elemental Analyzer for 13C for automated analysis of 13C in solids. This specific instrument provides fast analysis of 18O, while maintaining a high precision of 0.15 permil. This instrument, along with a current instrument used for 2H analysis, provides the optimal system for the analysis of energy expenditure by the doubly labeled water method. Our laboratory is one of the leading laboratories in the world for doubly labeled water analysis and has worked with dozens of users during the past two decades. We cannot, however, currently meet the requests from users due the age related down-time of our current 18O isotope ratio mass spectrometer and the absence of the automated inlet. This new instrument will dramatically improve increase our capacity to perform these isotope analyses and reduce our costs for the analyses. This new instrument will be housed in the Stable Isotope Laboratory of Nutritional Sciences at the University of Wisconsin. It will be operated under the direction Dr. Dale A. Schoeller and the operation of Timothy Shriver, MS. Oversight of the operation will provided by an executive committee composed of Drs. Colbert, Kemnitz and Schoeller to ensure equitable allotment of use among the users. The users group will consist of seven investigators. Four of these are from the University of Wisconsin and three from other US institutions. Most of the users will be utilizing the 18O analysis as it applies to doubly labeled water for the study of the etiology and treatment of obesity. Obesity remains the most prevalent and fastest growing health concerns in the United States. Obesity is among one of the top priorities on the NIH roadmap and the doubly labeled water method has been identified as one of the key research tools for the study o human obesity. In addition, this new instrument will provide new capacity in the form of the solids combustion inlet. This expands our potential users group in the area of development of dietary biomarkers.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：我们申请资金用于购买同位素比质谱仪进行18 O和13 C分析。我们选择了配备气体工作台II的Thermoelectron Delta Plus，用于CO2中18 O的自动分析，以及用于13 C的燃烧元素分析仪，用于固体中13 C的自动分析。这种特殊的仪器提供18 O的快速分析，同时保持0.15 permil的高精度。该仪器，沿着用于2 H分析的当前仪器，提供了用于通过双标记水方法分析能量消耗的最佳系统。我们的实验室是世界上双标记水分析的领先实验室之一，在过去的二十年里与数十名用户合作。然而，我们目前不能满足用户的要求，由于我们目前的18 O同位素比质谱仪的年龄相关的停机时间和自动入口的缺乏。这一新仪器将大大提高我们进行这些同位素分析的能力，并降低我们的分析成本。这台新仪器将被安置在威斯康星州大学营养科学稳定同位素实验室。它将在Dale A博士的指导下运作。Schoeller和Timothy Shriver女士的操作将由一个由Colbert博士、Kemnitz博士和Schoeller博士组成的执行委员会负责监督，以确保用户之间的公平分配。用户组将由7名调查员组成。其中四名来自威斯康星州大学，三名来自美国其他机构。大多数用户将使用18 O分析，因为它适用于双标记水，用于研究肥胖的病因和治疗。肥胖仍然是美国最普遍和增长最快的健康问题。肥胖是NIH路线图的首要任务之一，双标记水方法已被确定为研究人类肥胖的关键研究工具之一。此外，这种新仪器将以固体燃烧入口的形式提供新的容量。这扩大了我们在饮食生物标志物开发领域的潜在用户群。