ISOTOPE RATIO MASS SPECTROMETER: NUTRITION

同位素比质谱仪：营养

• 批准号: 7335004
• 负责人: DALE A SCHOELLER
• 金额: $ 6.5万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335004
• 关键词: fasting; nutrition; obesity; university; water

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We request funds for the purchase of an isotope ratio mass spectrometer for 18O and 13C analysis. We have selected the Thermoelectron Delta Plus equipped with a Gas Bench II for automated 18O analysis in CO2and a Combustion Elemental Analyzer for 13C for automated analysis of 13C in solids. This specific instrument provides fast analysis of 18O, while maintaining a high precision of 0.15 permil. This instrument, along with a current instrument used for 2H analysis, provides the optimal system for the analysis of energy expenditure by the doubly labeled water method. Our laboratory is one of the leading laboratories in the world for doubly labeled water analysis and has worked with dozens of users during the past two decades. We cannot, however, currently meet the requests from users due the age related down-time of our current 18O isotope ratio mass spectrometer and the absence of the automated inlet. This new instrument will dramatically improve increase our capacity to perform these isotope analyses and reduce our costs for the analyses. This new instrument will be housed in the Stable Isotope Laboratory of Nutritional Sciences at the University of Wisconsin. It will be operated under the direction Dr. Dale A. Schoeller and the operation of Timothy Shriver, MS. Oversight of the operation will provided by an executive committee composed of Drs. Colbert, Kemnitz and Schoeller to ensure equitable allotment of use among the users. The users group will consist of seven investigators. Four of these are from the University of Wisconsin and three from other US institutions. Most of the users will be utilizing the 18O analysis as it applies to doubly labeled water for the study of the etiology and treatment of obesity. Obesity remains the most prevalent and fastest growing health concerns in the United States. Obesity is among one of the top priorities on the NIH roadmap and the doubly labeled water method has been identified as one of the key research tools for the study o human obesity. In addition, this new instrument will provide new capacity in the form of the solids combustion inlet. This expands our potential users group in the area of development of dietary biomarkers.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：我们请求资金购买用于 18O 和 13C 分析的同位素比质谱仪。我们选择了配备有用于自动分析 CO2 中 18O 的 Gas Bench II 和用于自动分析固体中 13C 的 13C 燃烧元素分析仪的 Thermoelectron Delta Plus。该特定仪器可快速分析 18O，同时保持 0.15 permil 的高精度。该仪器与当前用于 2H 分析的仪器一起，为通过双标记水法分析能量消耗提供了最佳系统。我们的实验室是世界领先的双标记水分析实验室之一，在过去二十年中与数十名用户合作。然而，由于我们现有的 18O 同位素比质谱仪因老化而导致停机且缺乏自动进样口，目前我们无法满足用户的要求。这种新仪器将极大地提高我们进行这些同位素分析的能力并降低我们的分析成本。该新仪器将安装在威斯康星大学营养科学稳定同位素实验室。它将在 Dale A. Schoeller 博士和 Timothy Shriver, MS 的指导下运营。由博士组成的执行委员会将负责监督该行动。 Colbert、Kemnitz 和 Schoeller 确保用户之间公平分配使用。用户组将由七名调查员组成。其中四名来自威斯康星大学，三名来自其他美国机构。大多数用户将利用 18O 分析，因为它适用于双标记水来研究肥胖的病因和治疗。肥胖仍然是美国最普遍和增长最快的健康问题。肥胖是 NIH 路线图上的首要任务之一，双标记水法已被确定为研究人类肥胖的关键研究工具之一。此外，这种新仪器将以固体燃烧入口的形式提供新的能力。这扩大了我们在膳食生物标志物开发领域的潜在用户群。