Isotope Ratio Mass Spectrometer

描述（由申请人提供）：我们申请资金购买一台同位素比质谱仪用于18O和13C分析。我们选择了配备气体工作台II的热电子Delta Plus，用于二氧化碳中的18O自动分析，以及用于13C的燃烧元素分析仪，用于固体中的13C自动分析。这种特殊的仪器提供快速分析18O，同时保持高精度的0.15 peril。该仪器与目前用于2H分析的仪器一起，为双标记水法分析能量消耗提供了最佳系统。我们的实验室是世界上双标签水分析的领先实验室之一，在过去的二十年里与数十个用户合作。然而，我们目前无法满足用户的要求，因为我们现有的18O同位素比质谱仪的停机时间与年龄有关，而且没有自动进气口。这台新仪器将大大提高我们进行这些同位素分析的能力，并降低我们的分析成本。这台新仪器将被安置在威斯康星大学营养科学稳定同位素实验室。它将在Dale A. Schoeller博士和Timothy Shriver女士的指导下运作。Colbert， Kemnitz和Schoeller确保用户之间公平分配使用。用户组将由7名调查人员组成。其中4名来自威斯康星大学，3名来自其他美国院校。大多数用户将使用18O分析，因为它适用于双重标签水，用于研究肥胖的病因和治疗。在美国，肥胖仍然是最普遍、增长最快的健康问题。肥胖是美国国立卫生研究院路线图上的首要任务之一，双标签水法已被确定为研究人类肥胖的关键研究工具之一。此外，这种新仪器将以固体燃烧入口的形式提供新的容量。这扩大了我们在饮食生物标志物开发领域的潜在用户群体。

DESCRIPTION (provided by applicant): We request funds for the purchase of an isotope ratio mass spectrometer for 18O and 13C analysis. We have selected the Thermoelectron Delta Plus equipped with a Gas Bench II for automated 18O analysis in CO2and a Combustion Elemental Analyzer for 13C for automated analysis of 13C in solids. This specific instrument provides fast analysis of 18O, while maintaining a high precision of 0.15 permil. This instrument, along with a current instrument used for 2H analysis, provides the optimal system for the analysis of energy expenditure by the doubly labeled water method. Our laboratory is one of the leading laboratories in the world for doubly labeled water analysis and has worked with dozens of users during the past two decades. We cannot, however, currently meet the requests from users due the age related down-time of our current 18O isotope ratio mass spectrometer and the absence of the automated inlet. This new instrument will dramatically improve increase our capacity to perform these isotope analyses and reduce our costs for the analyses. This new instrument will be housed in the Stable Isotope Laboratory of Nutritional Sciences at the University of Wisconsin. It will be operated under the direction Dr. Dale A. Schoeller and the operation of Timothy Shriver, MS. Oversight of the operation will provided by an executive committee composed of Drs. Colbert, Kemnitz and Schoeller to ensure equitable allotment of use among the users. The users group will consist of seven investigators. Four of these are from the University of Wisconsin and three from other US institutions. Most of the users will be utilizing the 18O analysis as it applies to doubly labeled water for the study of the etiology and treatment of obesity. Obesity remains the most prevalent and fastest growing health concerns in the United States. Obesity is among one of the top priorities on the NIH roadmap and the doubly labeled water method has been identified as one of the key research tools for the study o human obesity. In addition, this new instrument will provide new capacity in the form of the solids combustion inlet. This expands our potential users group in the area of development of dietary biomarkers.