BECTON DICKENSON FACSARIA CELL SORTER: IMMUNOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：我们正在申请资金购买一个Becton Dickenson FACSAria高速细胞分选机。所要求的配置包括三个激光器，能够检测多达九种不同的荧光染料，具有一系列激发和发射特性。该仪器还包括单个分选细胞的收集和温度控制功能。新仪器将用于一些分析和分类应用程序，以支持NIH资助的项目。这些应用包括细胞信号分子的多重免疫荧光分析、动物组织中白细胞的分选以及酵母展示文库中高亲和力抗体和多肽的筛选。新仪器将放置在LJBI流式细胞仪设施中。已经制定了准入和使用政策，并作出了机构承诺，在最初三年的运作中为人员和维护提供支助。FACSAria在LJBI先进的流式细胞术开发和应用能力的发展中发挥着关键作用。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a Becton Dickenson FACSAria high speed cell sorter. The requested configuration includes three lasers, enabling the detection as many nine different fluorochromes with a range of excitation and emission properties. The instrument also includes capabilities for the collection and temperature control of single sorted cells. The new instrument will be used for a number of analysis and sorting applications in support of NIH-funded projects. These applications include multiplexed immunofluorescence analysis of cell signaling molecules, sorting of leukocytes from animal tissue, and the screening of yeast display libraries for high affinity antibodies and polypeptides. The new instrument will be housed in the LJBI Flow Cytometry Facility. Policies for access and use have been formulated, and an institutional commitment is in place to support personnel and maintenance for the initial three years of operation. The FACSAria plays a key role in the development of an advanced flow cytometry development and application capability at LJBI.