Nanotube and nanogold to gear phi29 DNA packaging motor

纳米管和纳米金齿轮 phi29 DNA 包装电机

• 批准号: 7245847
• 负责人: PEIXUAN GUO
• 金额: $ 25.93万
• 依托单位: UNIVERSITY OF CINCINNATI
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7245847
• 关键词: Bacteriophages; Binding; Biological; Biology; Biomimetics; Chemicals; Complex; DNA; DNA Packaging; DNA Sequence; Data Storage and Retrieval; Devices; Diagnosis; Disease; Disruption; Drug Delivery Systems; Engineering; Facility Construction Funding Category; In Vitro; International Aspects; Link; Mechanics; Medicine; Membrane; Modification; Motor; Movement; Nanotechnology; Nanotubes; Organism; Pattern; Phase; Process; Protein Array; Pump; RNA; Recombinant Proteins; Recombinants; Route; Signal Transduction; Staging; Structure; System; chemical group; density; design; dimer; in vivo; infancy; molecular sieving; nanoGold; nanodevice; nanomachine; nanomaterials; nanometer; nanoparticle; nanoscale; novel; pathogen

DESCRIPTION (provided by applicant): Nanotechniques involve the creation, characterization, and modification of organized nanomaterials to serve as building blocks for the construction of large-scale devices and systems. Living systems contain a wide variety of nanomachines and highly ordered structures, including motors, arrays, pumps, membrane cores, and valves. The novelty and ingenious design of bacterial virus phi29 DNA packaging motor, the strongest biomotor studied to date, have inspired the synthesis of this motor and its components as biomemitics for nanodevices. This 30 nm nanomotor uses six ATP-binding pRNA molecules to gear the motor. An imitative motor has been successfully constructed using purified recombinant proteins and artificially synthesized RNA. It can be turned on and off at will. The formation of ordered structural arrays of the motor complex and its components, the retention of motor function after 3'-end extension of the pRNA, and the ease with which pRNA dimers and trimers can be manipulated combine to make the RNA-containing motor a viable option as mechanical parts in nanotechnology. The long-term objective is to utilize this synthesized motor, together with arrays of its components, as parts in nanodevices, with several major applications: 1) To use this artificial motor as parts for nanodevices, such as apparatuses for in vivo drug delivery. 2) To use the ordered arrays of motor components as building facades for large-scale supramolecular structures to serve as molecular sieves, chips for the diagnosis of diseases, or as ultrahigh density data storage systems. The presence of six pRNA subunits in the hexameric building blocks will allow for the construction of arrays with multiple functions, for example to allow for the separate identification of multiple pathogens. 3) To develop this nanomotor into a DNA-sequencing apparatus, since the DNA-packaging process involves movement of the DNA through a 3.6-nm pore surrounded by six RNA that can be modified to accept chemical signals. Though all of these long-term applications are becoming more and more realizable, practical nanotechnology is still in its infancy. It would be unrealistic to propose applying the phi29 motor system directly to nanodevices in only three or four years. Thus, the short-term objective of this proposal is to construct pRNA or protein arrays that will serve as templates for the construction of patterned supramolecular structures, and to find the best routes through which to link nanotubes and nanogolds as well as to attach biological moieties and chemical groups to the motor or the constructed arrays, pRNA dimers, trimers and hexamers will be used for array construction. Chief among early concerns will be avoiding disruption of the assembly and functioning of the motor and/or arrays due to the incorporation of foreign components. This phase of the study will pave the way toward direct and practical technological applications of the motor and its arrays in nanodevices.

描述（由申请人提供）：纳米技术涉及有组织纳米材料的创建、表征和修饰，以作为构建大规模设备和系统的构建模块。生命系统包含各种各样的纳米机器和高度有序的结构，包括马达、阵列、泵、膜芯和阀门。细菌病毒phi 29 DNA包装马达是迄今为止研究的最强的生物马达，其新奇和巧妙的设计激发了这种马达及其组件作为纳米器件的仿生学的合成。这种30 nm的纳米粒子使用六个ATP结合的pRNA分子来驱动马达。利用纯化的重组蛋白和人工合成的RNA成功地构建了一个模拟马达。它可以随意打开和关闭。马达复合物及其组分的有序结构阵列的形成、pRNA的3 '端延伸后马达功能的保留、以及pRNA二聚体和三聚体可被操纵的容易性联合收割机使含RNA马达成为纳米技术中作为机械部件的可行选择。 长期目标是利用这种合成马达及其组件阵列作为纳米器件中的部件，具有几个主要应用：1）使用这种人造马达作为纳米器件的部件，例如用于体内药物递送的装置。2)利用马达组件的有序阵列作为大规模超分子结构的建筑立面，作为分子筛、疾病诊断芯片或高密度数据存储系统。六聚体结构单元中六个pRNA亚基的存在将允许构建具有多种功能的阵列，例如允许单独鉴定多种病原体。3)为了将这种纳米管开发成DNA测序仪器，因为DNA包装过程涉及DNA通过3.6 nm孔的移动，该孔被六种RNA包围，这些RNA可以被修饰以接受化学信号。 虽然所有这些长期应用正变得越来越现实，实用的纳米技术仍处于起步阶段。在短短三四年内，将phi 29电机系统直接应用于纳米器件是不现实的。因此，该提案的短期目标是构建pRNA或蛋白质阵列，其将用作构建图案化超分子结构的模板，并找到连接纳米管和纳米金以及将生物部分和化学基团连接到马达或构建的阵列的最佳途径。pRNA二聚体、三聚体和六聚体将用于阵列构建。早期关注的主要问题将是避免由于引入外来组件而破坏电机和/或阵列的组装和功能。这一阶段的研究将为电机及其阵列在纳米器件中的直接和实际技术应用铺平道路。