Olympus Fluoview 1000 Confocal Microscope

描述(由申请人提供)：在组织、细胞和分子水平上分析基因功能需要使用最先进、复杂的分析工具。沙漠山生物实验室(MDIBL)正在积极开展全年和季节性的科学计划，为比较功能基因组学做出重大贡献。为了加强和继续这些研究项目的成功，MDIBL的研究人员需要获得当前共焦显微镜平台上可用的定量、精细成像技术。这项申请得到了17名NIH资助的研究人员的支持，旨在寻求支持或购买基本的单光子奥林巴斯FluoView 1000共焦显微镜。该仪器将被安置在成像中心的一座新的最先进的实验室大楼内。新的共聚焦显微镜将有助于美国第一个海洋功能基因组研究中心(CMFGS)的扩大，并有助于NIH资助的与该中心相关的研究项目的发展。CMFGS的目标是利用海洋模型来利用比较方法来理解基因组序列和生物功能之间的关系，并将这种理解应用于改善人类健康。 共焦显微镜是成像中心提供的一项基本服务；当前的中心依赖于第一代奥林巴斯FluoView 333，它能够进行双色、双通道成像，几乎已有10年的历史，当然不再代表当前的技术。因此，这项提议的目标是：1)取代不再用作核心显微镜的9.5年的奥林巴斯仪器；2)提供急需的仪器时间；以及3)提供所需的共焦技术，特别选择405和440 nm激发和3通道检测。所要求的显微镜将通过提供最先进的仪器来促进比较功能基因组研究中的多学科研究，从而促进国家对生物医学研究和培训的健康需求。这项研究将探索海洋基因组序列和生物功能之间的关系，并将这种理解应用于人类健康，特别是在毒理学和涉及膜生物学和运输的疾病方面。

DESCRIPTION (provided by applicant): The analysis of gene function at the tissue, cellular and molecular level requires access to state-of-the-art, sophisticated analytic tools. The Mount Desert Island Biological Laboratory (MDIBL) is making significant contributions to comparative functional genomics with active year-round and seasonal scientific programs. To enhance and continue the success of these research programs, MDIBL investigators need access to the quantitative, fine scale imaging technologies available in current confocal microscopy platforms. This application, which is supported by 17 NIH-funded investigators, seeks support or the purchase of a basic single photon Olympus Fluoview 1000 confocal microscope. The instrument will be housed in the Imaging Center within a new state-of-the-art laboratory building. The new confocal microscope will contribute to the expansion of the nation's first Center for Marine Functional Genomic Studies (CMFGS) and to the development of NIH-funded research programs associated with this Center. The goals of the CMFGS are to exploit comparative approaches using marine models to understand relationships between genomic sequence and biological function, and to apply this understanding to improve human health. Confocal microscopy is an essential service provided by the Imaging Center; the current Center depends upon a first generation Olympus Fluoview 333 capable of two color, two channel imaging which is almost 10 years old, and certainly no longer represents current technology. Thus, the goals of this proposal are to: 1) Replace the 9.5 year old Olympus instrument which is no longer serviceable as a core microscope; 2) Provide critically needed instrument time; and 3) Provide access to requested confocal technologies, specifically selected 405 and 440 nm excitation and 3 channel detection. The requested microscope will contribute to national health needs for biomedical research and training by providing state-of-the-art instrumentation to facilitate multidisciplinary research in comparative functional genomic studies. This research will exploit relationships between marine genomic sequence and biological function, and will apply this understanding to human health, particularly in toxicology and in diseases involving membrane biology and transport.