Raman Tomography of Musculoskeletal Tissue

肌肉骨骼组织的拉曼断层扫描

• 批准号: 7300612
• 负责人: MICHAEL DAVID MORRIS
• 金额: $ 29.43万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7300612
• 关键词: Algorithms; Analytical Chemistry; Animals; Anterior; Area; Arterial Fatty Streak; Arthroplasty; Bone Diseases; Bone Tissue; Carbonates; Cholesterol; Clinic Visits; Collagen Fibril; Collection; Complex; Consent; DEXA; Defect; Depth; Distal; Family suidae; Fiber; Fiber Optics; Fracture; Fracture Healing; Healed; Health; Human; Image; Imaging Techniques; Invasive; Knee joint; Lasers; Life; Ligaments; Light; Limb structure; Maintenance; Measurement; Measures; Minerals; Modeling; Mus; Musculoskeletal; Operating Room Technicians; Operating Rooms; Operative Surgical Procedures; Optics; Orthopedics; Osteogenesis Imperfecta; Patients; Penetration; Pharmacologic Substance; Procedures; Process; Public Health; Quality Indicator; Raman Spectrum Analysis; Recovery; Replacement Arthroplasty; Research Personnel; Resolution; Risk; Safety; Schedule; Serum; Shapes; Signal Transduction; Site; Skin; Specimen; Spectrum Analysis; Sterility; Stress Fractures; Sus scrofa; Techniques; Technology; Tendon structure; Testing; Time; Tissues; Validation; Week; Wild Type Mouse; animal tissue; bone; bone imaging; bone quality; crosslink; design; detector; experience; fluorescence imaging; healing; human subject; human tissue; inorganic phosphate; instrument; interest; mouse model; new technology; novel; optical fiber; programs; prototype; radius bone structure; research study; spectroscopic imaging; tibia; tissue regeneration; tomography

DESCRIPTION (provided by applicant): We will develop Raman tomography, a non-invasive platform technology, for the recovery of spatial and spectral information from live animals and human subjects. Raman tomography will provide measurements of bone quality at depths of up to 1 cm below the skin. Available quality indicators will include mineral/matrix ratio, mineral crystallinity and carbonate/phosphate ratio and collagen-fibril cross-link state. The technology requires only a CW laser, an imaging spectrograph and CCD detector. Two specially designed fiber optic probes that provide shaped and distributed laser light to excite spectra and an array of 50 or more collection fibers to recover spectra will be used. Guided by tissue optics measurements, a ring/disk probe will be developed for rapid, deep tissue spectroscopy. Similarly, a tomographic probe will be developed for recovery of spectroscopic images. Modified versions of proven chemometric and tomographic signal recovery algorithms will be used to recover spectra and bone quality indicators. We will investigate trade-offs among signal acquisition time, depth penetration and spatial/spectral detail. Optimized probes will be used to study fracture healing in wild type mice and brtl, a model of osteogenesis imperfecta type IV.. Studies will be performed on live anaesthetized mice and on excised limbs from animals sacrificed at four time points in the eight week tissue regeneration period. Excised limbs allow validation by direct measurement of dissected, sectioned tissue. Porcine models will be used as models for human subjects, because of similarities of skin and tendon optics, allowing progression to Raman tomography of cadaveric tibial and distal radius tissue. Finally, we will demonstrate application to human subjects using arthroplasty patients whose bone tissue is exposed during surgery, allowing measurement validation. Although the focus of this project is on bone tissue, the technology is broadly applicable. Relevance to public health: Raman tomography provides new information for assessment of bone quality and fracture risk unavailable by DEXA or micro-MRI. The instruments used are small, safe and non-invasive, requiring only that a laser light be shined on the subject's skin. The technology is also readily adapted to measurement of other important health indicators, including presence and extent of atherosclerotic plaque and levels of serum cholesterol.

描述（由申请人提供）：我们将开发一种非侵入性平台技术拉曼断层扫描，以恢复活动物和人类受试者的空间和光谱信息。拉曼断层扫描将在皮肤以下最多1厘米的深度提供骨质质量的测量。可用的质量指标将包括矿物/基质比，矿物质结晶度和碳酸盐/磷酸盐比和胶原 - 纤维纤维交联态。该技术仅需要CW激光器，成像光谱仪和CCD检测器。将使用两种专门设计的光纤探针，可提供形状和分布的激光灯以激发光谱，并使用50或更多收集纤维的阵列来恢复光谱。在组织光学测量的指导下，将开发一个环/磁盘探针，用于快速，深层组织光谱。同样，将开发层析成像探针以恢复光谱图像。经过验证的化学计量和断层信号恢复算法的修改版本将用于恢复光谱和骨质质量指标。我们将研究信号获取时间，深度渗透和空间/光谱细节之间的权衡。优化的探针将用于研究野生型小鼠和BRTL的骨折愈合，这是IVECTA型不完美的肌生成模型。研究将对活的麻醉小鼠进行研究，并在八周的组织再生期间在四个时间点牺牲的动物中脱离动物的肢体。切除的四肢允许通过直接测量解剖的截面组织进行验证。由于皮肤和肌腱光学的相似性，猪模型将用作人类受试者的模型，从而使尸体胫骨和远端半径组织的拉曼层析成像进展。最后，我们将使用关节置换术患者对人类受试者进行应用，其骨组织在手术过程中暴露，从而允许测量验证。尽管该项目的重点是骨组织，但该技术广泛适用。 与公共卫生的相关性：拉曼断层扫描提供了新信息，以评估DEXA或Micro-MRI无法获得骨质质量和骨折风险。所使用的仪器是小的，安全的和无创的，只需要在受试者的皮肤上发光。该技术也很容易适应其他重要的健康指标的测量，包括动脉粥样硬化斑块的存在和程度和血清胆固醇水平。