Raman Tomography of Musculoskeletal Tissue

肌肉骨骼组织的拉曼断层扫描

• 批准号: 7300612
• 负责人: MICHAEL DAVID MORRIS
• 金额: $ 29.43万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7300612
• 关键词: Algorithms; Analytical Chemistry; Animals; Anterior; Area; Arterial Fatty Streak; Arthroplasty; Bone Diseases; Bone Tissue; Carbonates; Cholesterol; Clinic Visits; Collagen Fibril; Collection; Complex; Consent; DEXA; Defect; Depth; Distal; Family suidae; Fiber; Fiber Optics; Fracture; Fracture Healing; Healed; Health; Human; Image; Imaging Techniques; Invasive; Knee joint; Lasers; Life; Ligaments; Light; Limb structure; Maintenance; Measurement; Measures; Minerals; Modeling; Mus; Musculoskeletal; Operating Room Technicians; Operating Rooms; Operative Surgical Procedures; Optics; Orthopedics; Osteogenesis Imperfecta; Patients; Penetration; Pharmacologic Substance; Procedures; Process; Public Health; Quality Indicator; Raman Spectrum Analysis; Recovery; Replacement Arthroplasty; Research Personnel; Resolution; Risk; Safety; Schedule; Serum; Shapes; Signal Transduction; Site; Skin; Specimen; Spectrum Analysis; Sterility; Stress Fractures; Sus scrofa; Techniques; Technology; Tendon structure; Testing; Time; Tissues; Validation; Week; Wild Type Mouse; animal tissue; bone; bone imaging; bone quality; crosslink; design; detector; experience; fluorescence imaging; healing; human subject; human tissue; inorganic phosphate; instrument; interest; mouse model; new technology; novel; optical fiber; programs; prototype; radius bone structure; research study; spectroscopic imaging; tibia; tissue regeneration; tomography

DESCRIPTION (provided by applicant): We will develop Raman tomography, a non-invasive platform technology, for the recovery of spatial and spectral information from live animals and human subjects. Raman tomography will provide measurements of bone quality at depths of up to 1 cm below the skin. Available quality indicators will include mineral/matrix ratio, mineral crystallinity and carbonate/phosphate ratio and collagen-fibril cross-link state. The technology requires only a CW laser, an imaging spectrograph and CCD detector. Two specially designed fiber optic probes that provide shaped and distributed laser light to excite spectra and an array of 50 or more collection fibers to recover spectra will be used. Guided by tissue optics measurements, a ring/disk probe will be developed for rapid, deep tissue spectroscopy. Similarly, a tomographic probe will be developed for recovery of spectroscopic images. Modified versions of proven chemometric and tomographic signal recovery algorithms will be used to recover spectra and bone quality indicators. We will investigate trade-offs among signal acquisition time, depth penetration and spatial/spectral detail. Optimized probes will be used to study fracture healing in wild type mice and brtl, a model of osteogenesis imperfecta type IV.. Studies will be performed on live anaesthetized mice and on excised limbs from animals sacrificed at four time points in the eight week tissue regeneration period. Excised limbs allow validation by direct measurement of dissected, sectioned tissue. Porcine models will be used as models for human subjects, because of similarities of skin and tendon optics, allowing progression to Raman tomography of cadaveric tibial and distal radius tissue. Finally, we will demonstrate application to human subjects using arthroplasty patients whose bone tissue is exposed during surgery, allowing measurement validation. Although the focus of this project is on bone tissue, the technology is broadly applicable. Relevance to public health: Raman tomography provides new information for assessment of bone quality and fracture risk unavailable by DEXA or micro-MRI. The instruments used are small, safe and non-invasive, requiring only that a laser light be shined on the subject's skin. The technology is also readily adapted to measurement of other important health indicators, including presence and extent of atherosclerotic plaque and levels of serum cholesterol.

描述(申请人提供)：我们将开发拉曼断层扫描，这是一种非侵入性的平台技术，用于从活体动物和人类受试者中恢复空间和光谱信息。拉曼断层扫描将提供皮肤下1厘米深处的骨骼质量测量。可用的质量指标将包括矿物/基质比、矿物结晶度、碳酸盐/磷酸盐比和胶原纤维交联态。这项技术只需要一台连续波激光器、一台成像光谱仪和一台CCD探测器。将使用两个专门设计的光纤探头，提供定形和分布的激光来激发光谱，以及一个由50根或更多收集光纤组成的阵列来恢复光谱。在组织光学测量的指导下，将开发一种环/盘探头，用于快速、深层组织光谱分析。同样，将开发一种用于恢复光谱图像的层析探头。经过验证的化学计量学和断层扫描信号恢复算法的修改版本将用于恢复光谱和骨骼质量指标。我们将研究信号采集时间、深度穿透和空间/光谱细节之间的权衡。优化后的探针将用于研究野生型小鼠的骨折愈合情况，以及IV型成骨不全模型BRTL。研究将在活体麻醉的小鼠身上进行，并在八周组织再生期的四个时间点处死动物的切除肢体上进行。切除的肢体可以通过直接测量解剖的切片组织来进行验证。猪模型将被用作人类受试者的模型，因为皮肤和肌腱的光学结构相似，因此可以进行身体胫骨和桡骨远端组织的拉曼断层扫描。最后，我们将使用骨组织在手术过程中暴露的关节成形术患者演示其在人类受试者中的应用，从而允许测量验证。虽然该项目的重点是骨组织，但该技术具有广泛的适用性。 与公众健康相关：拉曼断层扫描为评估骨骼质量和骨折风险提供了新的信息，这是DEXA或微型MRI无法提供的。所使用的仪器体积小、安全、非侵入性，只需要用激光照射受试者的皮肤即可。这项技术也很容易被用于测量其他重要的健康指标，包括动脉粥样硬化斑块的存在和程度以及血清胆固醇水平。