Core C: Analytical Core
核心 C:分析核心
基本信息
- 批准号:7158291
- 负责人:
- 金额:$ 9.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-07-01 至 2011-06-30
- 项目状态:已结题
- 来源:
- 关键词:analytical chemistryanimal tissueautismbiomarkerbiomedical facilitychildrendisease /disorder modelelectrospray ionization mass spectrometryenvironmental healthfamily geneticsgene environment interactiongenetic susceptibilityhalobiphenyl /halotriphenyl compoundhuman tissueimmunologic assay /testlipid metabolismliquid chromatography mass spectrometrymetabolomicsneurotoxicologypesticidesproteomicstryptophan
项目摘要
The overall objective of the Analytical Chemistry Core is to provide analytical support for the
human monitoring, animal model, and cell biology projects in the program project. We will apply
techniques to the research projects as appropriate, and will advance the technology upon which the
analytical methodology is based. This core will conduct new analyses on serum samples from the
CHARGE study, collected during the previous funding period, new samples from the CHARGEBACK
and MARBLES study (see Projects 1 and 2). We will evaluate new methods to apply to the
prospective study of future siblings and to test specific hypotheses. A major goal will be to generate
data sets of xenobiotic exposure and metabolomic biomarkers to regress against transcriptome and
proteome data by determining both xenobiotic exposure and levels of endogenous metabolites on
selected . We will evaluate metabolite profiles in animal models emphasizing immune dysfunction.
In human samples, we will pay particular attention to metabolites indicative of inflammatory status
and plasma leptin levels recently found as a biomarker to distinguish between early onset and
clinical regression autism. We also will monitor the metabolite profiles in T cells, B cells and natural
killer (NK) cells of normal and autistic children with and without exposure to xenobiotics. Of equal
importance we will provide a walk up instrument and consultation service in analytical chemistry for
the entire core the Specific Aims are:
1. Establish the metabolic pathways responsible for variations in lipid composition between autistic
children and their siblings and between normal and immunologically challenged animal models of
autism.
2. Use global metabolomic procedures to search for biomarkers of autism.
3. Provide analytical data on pesticide and other xenobiotic levels in cell and in vivo model systems
and in serum samples from the CHARGE, CHARGE-BACK, MARBLES, and other successor
studies.
4. Develop new analytical methods for xenobiotics of interest to scientists in the program project.
分析化学核心的总体目标是提供分析支持,
该计划项目中的人体监测、动物模型和细胞生物学项目。我们将应用
技术的研究项目,并将推进技术上,
分析方法是基础。该核心将对来自
CHARGE研究,在上一个资助期内收集,来自CHARGEBACK的新样本
和大理石研究(见项目1和2)。我们将评估适用于
未来的兄弟姐妹的前瞻性研究,并测试特定的假设。一个主要目标将是产生
异生物质暴露和代谢组学生物标志物的数据集,以针对转录组回归,
蛋白质组数据,通过确定外源性暴露和内源性代谢物水平,
选定.我们将在强调免疫功能障碍的动物模型中评估代谢产物谱。
在人类样本中,我们将特别注意指示炎症状态的代谢物
和血浆瘦素水平最近被发现作为区分早发性和
临床退化自闭症我们还将监测T细胞、B细胞和自然细胞中的代谢产物谱。
正常儿童和自闭症儿童的NK细胞,有或没有接触外源性物质。平等
重要性我们将提供一个步行了仪器和咨询服务,在分析化学,
具体目标的整个核心是:
1.建立负责自闭症患者之间脂质组成变化的代谢途径
儿童和他们的兄弟姐妹之间以及正常和免疫挑战的动物模型之间,
自闭症
2.使用全球代谢组学程序来寻找自闭症的生物标志物。
3.提供细胞和体内模型系统中农药和其他生物外源性物质水平的分析数据
以及来自CHARGE、CHARGE-BACK、MARBLES和其他后继者的血清样本
问题研究
4.为项目中的科学家开发感兴趣的外源性物质的新分析方法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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BRUCE D HAMMOCK其他文献
BRUCE D HAMMOCK的其他文献
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{{ truncateString('BRUCE D HAMMOCK', 18)}}的其他基金
Soluble epoxide hydrolase and epoxide fatty acid involvement in corneal injury after ammonia exposure: Mechanisms of injury and potential therapeutics using sEH inhibitors and biostable EpFA mimics.
可溶性环氧化物水解酶和环氧化物脂肪酸参与氨暴露后角膜损伤:损伤机制和使用 sEH 抑制剂和生物稳定 EpFA 模拟物的潜在治疗方法。
- 批准号:
10708436 - 财政年份:2023
- 资助金额:
$ 9.47万 - 项目类别:
Bioactive lipids as effectors and indicators of the deleterious effects of environmental exposure on chronic diseases
生物活性脂质作为环境暴露对慢性疾病有害影响的效应物和指标
- 批准号:
10400036 - 财政年份:2019
- 资助金额:
$ 9.47万 - 项目类别:
Bioactive lipids as effectors and indicators of the deleterious effects of environmental exposure on chronic diseases
生物活性脂质作为环境暴露对慢性疾病有害影响的效应物和指标
- 批准号:
10615675 - 财政年份:2019
- 资助金额:
$ 9.47万 - 项目类别:
Bioactive lipids as effectors and indicators of the deleterious effects of environmental exposure on chronic diseases
生物活性脂质作为环境暴露对慢性疾病有害影响的效应物和指标
- 批准号:
10153794 - 财政年份:2019
- 资助金额:
$ 9.47万 - 项目类别:
Clinical Paths for Soluble Epoxide Hydrolase Inhibitors at Experimental Biology 2018
2018 年实验生物学中可溶性环氧化物水解酶抑制剂的临床路径
- 批准号:
9544621 - 财政年份:2018
- 资助金额:
$ 9.47万 - 项目类别:
Role of Epoxygenated Fatty Acids in Modulating Pain
环氧化脂肪酸在调节疼痛中的作用
- 批准号:
8446055 - 财政年份:2013
- 资助金额:
$ 9.47万 - 项目类别:
Role of Epoxygenated Fatty Acids in Modulating Pain
环氧化脂肪酸在调节疼痛中的作用
- 批准号:
8619587 - 财政年份:2013
- 资助金额:
$ 9.47万 - 项目类别:
METHODS MONITOR TOXIC SUBSTAN AND/OR INDICATORS OF PRESENCE IN HUMANS&OTHER SPE
监测人类体内有毒物质和/或存在指标的方法
- 批准号:
8362756 - 财政年份:2011
- 资助金额:
$ 9.47万 - 项目类别:
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