Core C: Analytical Core

核心 C：分析核心

• 批准号: 7158291
• 负责人: BRUCE D HAMMOCK
• 金额: $ 9.47万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7158291
• 关键词: analytical chemistry; animal tissue; autism; biomarker; biomedical facility; children; disease /disorder model; electrospray ionization mass spectrometry; environmental health; family genetics; gene environment interaction; genetic susceptibility; halobiphenyl /halotriphenyl compound; human tissue; immunologic assay /test; lipid metabolism; liquid chromatography mass spectrometry; metabolomics; neurotoxicology; pesticides; proteomics; tryptophan

The overall objective of the Analytical Chemistry Core is to provide analytical support for the human monitoring, animal model, and cell biology projects in the program project. We will apply techniques to the research projects as appropriate, and will advance the technology upon which the analytical methodology is based. This core will conduct new analyses on serum samples from the CHARGE study, collected during the previous funding period, new samples from the CHARGEBACK and MARBLES study (see Projects 1 and 2). We will evaluate new methods to apply to the prospective study of future siblings and to test specific hypotheses. A major goal will be to generate data sets of xenobiotic exposure and metabolomic biomarkers to regress against transcriptome and proteome data by determining both xenobiotic exposure and levels of endogenous metabolites on selected . We will evaluate metabolite profiles in animal models emphasizing immune dysfunction. In human samples, we will pay particular attention to metabolites indicative of inflammatory status and plasma leptin levels recently found as a biomarker to distinguish between early onset and clinical regression autism. We also will monitor the metabolite profiles in T cells, B cells and natural killer (NK) cells of normal and autistic children with and without exposure to xenobiotics. Of equal importance we will provide a walk up instrument and consultation service in analytical chemistry for the entire core the Specific Aims are: 1. Establish the metabolic pathways responsible for variations in lipid composition between autistic children and their siblings and between normal and immunologically challenged animal models of autism. 2. Use global metabolomic procedures to search for biomarkers of autism. 3. Provide analytical data on pesticide and other xenobiotic levels in cell and in vivo model systems and in serum samples from the CHARGE, CHARGE-BACK, MARBLES, and other successor studies. 4. Develop new analytical methods for xenobiotics of interest to scientists in the program project.

分析化学核心的总体目标是提供分析支持， 该计划项目中的人体监测、动物模型和细胞生物学项目。我们将应用 技术的研究项目，并将推进技术上， 分析方法是基础。该核心将对来自 CHARGE研究，在上一个资助期内收集，来自CHARGEBACK的新样本 和大理石研究（见项目1和2）。我们将评估适用于 未来的兄弟姐妹的前瞻性研究，并测试特定的假设。一个主要目标将是产生 异生物质暴露和代谢组学生物标志物的数据集，以针对转录组回归， 蛋白质组数据，通过确定外源性暴露和内源性代谢物水平， 选定.我们将在强调免疫功能障碍的动物模型中评估代谢产物谱。 在人类样本中，我们将特别注意指示炎症状态的代谢物 和血浆瘦素水平最近被发现作为区分早发性和 临床退化自闭症我们还将监测T细胞、B细胞和自然细胞中的代谢产物谱。 正常儿童和自闭症儿童的NK细胞，有或没有接触外源性物质。平等 重要性我们将提供一个步行了仪器和咨询服务，在分析化学， 具体目标的整个核心是： 1.建立负责自闭症患者之间脂质组成变化的代谢途径 儿童和他们的兄弟姐妹之间以及正常和免疫挑战的动物模型之间， 自闭症 2.使用全球代谢组学程序来寻找自闭症的生物标志物。 3.提供细胞和体内模型系统中农药和其他生物外源性物质水平的分析数据 以及来自CHARGE、CHARGE-BACK、MARBLES和其他后继者的血清样本 问题研究 4.为项目中的科学家开发感兴趣的外源性物质的新分析方法。