Infrared Laser Desorption Ion Mobility Mass Spectrometer

红外激光解吸离子淌度质谱仪

• 批准号: 7273987
• 负责人: John Albert Schultz
• 金额: $ 19.94万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7273987
• 关键词: Acceleration; Aplysia; Bacteria; Biological; Biological Markers; Biomedical Research; Brain; Cell Mobility; Cerebrospinal Fluid; Charge; Chemicals; Clinical; Collaborations; Collection; Communities; Complex; Computer software; Data; Detection; Development; Diagnostic; Disease; Drug Addiction; Engineering; Funding; Generations; Goals; Grant; Height; Image; Ions; Laboratory Rat; Lasers; Legal patent; Lipids; Louisiana; Maps; Mass Spectrum Analysis; Mental disorders; Methods; Modeling; National Institute of Drug Abuse; Nature; Neuropeptides; Noise; Peptides; Performance; Phase; Physiologic pulse; Positioning Attribute; Postdoctoral Fellow; Problem Solving; Process; Proteins; Proteomics; Pulse Rates; Pulse taking; Pump; Rattus; Research; Research Personnel; Resolution; Sampling; Signal Transduction; Slice; Solutions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Students; System; Techniques; Technology; Testing; Time; Tissues; United States National Institutes of Health; Universities; Wood material; Work; analog; base; brain tissue; commercial application; data acquisition; detector; digital; drug of abuse; env Gene Products; improved; insight; instrument; interest; ion mobility; ionization; mass spectrometer; novel; programs; small molecule; tool; trend; two-dimensional; ultraviolet; voltage

DESCRIPTION (provided by applicant): The development of an infrared laser desorption ionization ion mobility mass spectrometer (IR LDI IM MS) will be continued. The analysis of test biomolecules such as peptides and proteins in the Phase I of the project has demonstrated that the new system's performance is not only comparable, but, in some aspects, superior to the UV MALDI mode due to both mobility pre-separation of ion and softer nature of the IR MALDI. These results could provide a breakthrough in both top down proteomics as well as protein biomarker identification. Matrix-free laser desorption as well as efficient two-dimensional analysis of rat brain tissues and bacteria was also demonstrated. Based on the proof of capabilities of the technique in the Phase I, the instrument will be applied to the analysis of biological tissues. Both matrix-assisted and matrix-free approach will be used. The instrument will be upgraded in order to further increase the throughput, sensitivity and resolution. As part of this research, Ionwerks is collaborating with the mass spectrometry research group of Prof. Kermit Murray at Louisiana State University who are the experts in the use of IR lasers for the direct analysis of biomolecules. The utility of the IR LDI IM MS system spatial imaging of drugs of abuse, metabolite, lipids, and neuropeptides on and within rat brain tissue sections and the model Aplysia californica will be demonstrated. An advanced technique of the analysis of complex biological systems will be developed, which will have significant advantages compared to the conventional UV MALDI. Important commercial applications of this technology are in biomedical research and clinical diagnostics.

描述（由申请人提供）：将继续开发红外激光解吸电离离子迁移质谱仪（IR LDI IM MS）。在项目的第一阶段中，对测试生物分子（如肽和蛋白质）的分析表明，新系统的性能不仅与UV MALDI模式相当，而且在某些方面上级UV MALDI模式，这是由于离子的迁移率预分离和IR MALDI的较软性质。这些结果可以在自上而下的蛋白质组学以及蛋白质生物标志物鉴定方面提供突破。无基质激光解吸以及有效的二维分析大鼠脑组织和细菌也被证明。基于第一阶段的技术能力证明，该仪器将应用于生物组织的分析。将使用矩阵辅助和无矩阵方法。将对该仪器进行升级，以进一步提高通量、灵敏度和分辨率。作为这项研究的一部分，Ionwerks正在与路易斯安那州州立大学的Kermit Murray教授的质谱研究小组合作，他们是使用红外激光直接分析生物分子的专家。IR LDI IM MS系统的滥用药物，代谢物，脂质和神经肽的空间成像的实用程序和大鼠脑组织切片和模型的加利福尼亚州阿龙将被证明。一种先进的技术，分析复杂的生物系统将开发，这将有显着的优势相比，传统的紫外MALDI。该技术的重要商业应用是生物医学研究和临床诊断。