Photofragmentation MS of Ion Mobility Separated Peptides

离子淌度分离肽的光裂解质谱

• 批准号: 6643145
• 负责人: John Albert Schultz
• 金额: $ 9.95万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6643145
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; high throughput technology; ion transport; mass spectrometry; matrix assisted laser desorption ionization; peptide structure; peptides; photoconduction

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project proposal is focused on the development of a commercial instrument capable of rapid, high-throughput identification of peptides based on matrix-assisted laser desorption ionization, ion mobility spectrometry, photodissociation, and time-of-flight mass spectrometry technology. The implications of the proposed instrument are vast but the most significant contribution is a time reduction in the identification of peptides from mixtures (e.g., protein digests). The keys to the project are the use of an ion mobility spectrometer as the separation device and a laser for ion dissociation. For example, an ion mobility spectrometer is capable of separating peptide mixtures with a resolution of 50-100 on the millisecond time-scale. This is a significant saving in time compared to the tens to hundreds of minutes needed by HPLC. The incorporation of a laser to induce fragmentation provides mass and chemical selectivity not afforded by other fragmentation means such as collision-induced dissociation or surface induced dissociation. Use of a laser also eliminates the need for numerous ion optical devices for fragmentation and focusing of ions, thereby simplifying the overall instrument design. The instrument can also be extended to other areas of chemical research simply by adjusting the laser wavelength.

描述（由申请人提供）：本小企业创新研究第一阶段项目提案的重点是开发一种商业仪器，能够基于基质辅助激光解吸电离，离子迁移谱，光解离和飞行时间质谱技术快速，高通量鉴定肽。所提出的仪器的影响是巨大的，但最重要的贡献是减少了从混合物中鉴定肽的时间（例如，蛋白酶）。该项目的关键是使用离子迁移谱仪作为分离装置和激光器进行离子解离。例如，离子迁移谱仪能够在毫秒时间尺度上以50-100的分辨率分离肽混合物。与HPLC所需的数十至数百分钟相比，这是一个显著的时间节省。引入激光以诱导碎裂提供了其它碎裂手段（例如碰撞诱导解离或表面诱导解离）所不能提供的质量和化学选择性。激光器的使用还消除了对用于离子的碎裂和聚焦的许多离子光学装置的需要，从而简化了整个仪器设计。该仪器还可以简单地通过调整激光波长扩展到化学研究的其他领域。