Photofragmentation MS of Ion Mobility Separated Peptides

描述(由申请人提供)：这项小型企业创新研究第一阶段项目提案专注于开发一种商业仪器，能够基于基质辅助激光解吸电离、离子迁移率光谱、光解离和飞行时间质谱学技术快速、高通量地鉴定多肽。拟议仪器的影响是巨大的，但最重要的贡献是缩短了从混合物(例如蛋白质消化)中鉴定多肽的时间。该项目的关键是使用离子迁移率光谱仪作为分离设备，并使用激光进行离子解离。例如，离子迁移率光谱仪能够在毫秒时间尺度上以50-100的分辨率分离多肽混合物。与高效液相色谱所需的几十到数百分钟相比，这是一个显着的节省时间。掺入激光诱导碎裂提供了其他碎裂方法所不具备的质量和化学选择性，例如碰撞诱导解离或表面诱导解离。激光的使用还消除了用于离子碎裂和聚焦的大量离子光学设备的需要，从而简化了整体仪器设计。通过调整激光波长，该仪器还可以扩展到化学研究的其他领域。

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project proposal is focused on the development of a commercial instrument capable of rapid, high-throughput identification of peptides based on matrix-assisted laser desorption ionization, ion mobility spectrometry, photodissociation, and time-of-flight mass spectrometry technology. The implications of the proposed instrument are vast but the most significant contribution is a time reduction in the identification of peptides from mixtures (e.g., protein digests). The keys to the project are the use of an ion mobility spectrometer as the separation device and a laser for ion dissociation. For example, an ion mobility spectrometer is capable of separating peptide mixtures with a resolution of 50-100 on the millisecond time-scale. This is a significant saving in time compared to the tens to hundreds of minutes needed by HPLC. The incorporation of a laser to induce fragmentation provides mass and chemical selectivity not afforded by other fragmentation means such as collision-induced dissociation or surface induced dissociation. Use of a laser also eliminates the need for numerous ion optical devices for fragmentation and focusing of ions, thereby simplifying the overall instrument design. The instrument can also be extended to other areas of chemical research simply by adjusting the laser wavelength.