Infrared Laser Desorption Ion Mobility Mass Spectrometer

描述(申请人提供)：将继续开发红外激光解吸电离离子迁移率质谱仪(IR LDI IM MS)。对项目第一阶段测试生物分子(如多肽和蛋白质)的分析表明，新系统的性能不仅与UV MALDI模式相当，而且在某些方面优于UV MALDI模式，这是因为离子的预先分离和IR MALDI的柔软性。这些结果可能为自上而下的蛋白质组学和蛋白质生物标记物的鉴定提供突破。还展示了无基质激光解吸以及对大鼠脑组织和细菌的高效二维分析。在第一阶段技术能力证明的基础上，该仪器将应用于生物组织的分析。将采用矩阵辅助和无矩阵两种方法。将对仪器进行升级，以进一步提高吞吐量、灵敏度和分辨率。作为这项研究的一部分，Ionwerks正在与路易斯安那州立大学Kermit Murray教授的质谱学研究小组合作，后者是使用红外激光直接分析生物分子的专家。将演示IR LDI IM MS系统对滥用药物、代谢物、脂质和神经肽在大鼠脑组织切片上和内的空间成像以及对海兔模型的应用。将开发一种分析复杂生物体系的先进技术，与传统的UV MALDI相比，这将具有显著的优势。这项技术的重要商业应用是生物医学研究和临床诊断。

DESCRIPTION (provided by applicant): The development of an infrared laser desorption ionization ion mobility mass spectrometer (IR LDI IM MS) will be continued. The analysis of test biomolecules such as peptides and proteins in the Phase I of the project has demonstrated that the new system's performance is not only comparable, but, in some aspects, superior to the UV MALDI mode due to both mobility pre-separation of ion and softer nature of the IR MALDI. These results could provide a breakthrough in both top down proteomics as well as protein biomarker identification. Matrix-free laser desorption as well as efficient two-dimensional analysis of rat brain tissues and bacteria was also demonstrated. Based on the proof of capabilities of the technique in the Phase I, the instrument will be applied to the analysis of biological tissues. Both matrix-assisted and matrix-free approach will be used. The instrument will be upgraded in order to further increase the throughput, sensitivity and resolution. As part of this research, Ionwerks is collaborating with the mass spectrometry research group of Prof. Kermit Murray at Louisiana State University who are the experts in the use of IR lasers for the direct analysis of biomolecules. The utility of the IR LDI IM MS system spatial imaging of drugs of abuse, metabolite, lipids, and neuropeptides on and within rat brain tissue sections and the model Aplysia californica will be demonstrated. An advanced technique of the analysis of complex biological systems will be developed, which will have significant advantages compared to the conventional UV MALDI. Important commercial applications of this technology are in biomedical research and clinical diagnostics.