Infrared Laser Desorption Ion Mobility Mass Spectrometer

红外激光解吸离子淌度质谱仪

• 批准号: 7463890
• 负责人: John Albert Schultz
• 金额: $ 19.41万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-10 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7463890
• 关键词: Acceleration; Aplysia; Bacteria; Biological; Biological Markers; Biomedical Research; Brain; Cell Mobility; Cerebrospinal Fluid; Charge; Chemicals; Clinical; Collaborations; Collection; Communities; Complex; Computer software; Data; Detection; Development; Diagnostic; Disease; Drug Addiction; Engineering; Funding; Generations; Goals; Grant; Height; Image; Ions; Laboratory Rat; Lasers; Legal patent; Lipids; Louisiana; Maps; Mass Spectrum Analysis; Mental disorders; Methods; Modeling; National Institute of Drug Abuse; Nature; Neuropeptides; Noise; Peptides; Performance; Phase; Physiologic pulse; Positioning Attribute; Postdoctoral Fellow; Problem Solving; Process; Proteins; Proteomics; Pulse Rates; Pulse taking; Pump; Rattus; Research; Research Personnel; Resolution; Sampling; Signal Transduction; Slice; Solutions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Students; System; Techniques; Technology; Testing; Time; Tissues; United States National Institutes of Health; Universities; Wood material; Work; analog; base; brain tissue; commercial application; data acquisition; detector; digital; drug of abuse; env Gene Products; improved; insight; instrument; interest; ion mobility; ionization; mass spectrometer; novel; programs; small molecule; tool; trend; two-dimensional; ultraviolet; voltage

DESCRIPTION (provided by applicant): The development of an infrared laser desorption ionization ion mobility mass spectrometer (IR LDI IM MS) will be continued. The analysis of test biomolecules such as peptides and proteins in the Phase I of the project has demonstrated that the new system's performance is not only comparable, but, in some aspects, superior to the UV MALDI mode due to both mobility pre-separation of ion and softer nature of the IR MALDI. These results could provide a breakthrough in both top down proteomics as well as protein biomarker identification. Matrix-free laser desorption as well as efficient two-dimensional analysis of rat brain tissues and bacteria was also demonstrated. Based on the proof of capabilities of the technique in the Phase I, the instrument will be applied to the analysis of biological tissues. Both matrix-assisted and matrix-free approach will be used. The instrument will be upgraded in order to further increase the throughput, sensitivity and resolution. As part of this research, Ionwerks is collaborating with the mass spectrometry research group of Prof. Kermit Murray at Louisiana State University who are the experts in the use of IR lasers for the direct analysis of biomolecules. The utility of the IR LDI IM MS system spatial imaging of drugs of abuse, metabolite, lipids, and neuropeptides on and within rat brain tissue sections and the model Aplysia californica will be demonstrated. An advanced technique of the analysis of complex biological systems will be developed, which will have significant advantages compared to the conventional UV MALDI. Important commercial applications of this technology are in biomedical research and clinical diagnostics.

描述（由申请人提供）：将继续开发红外激光解吸电离离子迁移质谱计（IR LDI IM MS）。该项目的第一期测试生物分子（如多肽和蛋白质）的分析表明，由于离子的迁移性和IR MALDI的柔软性，新系统的性能不仅与UV MALDI模式相当，而且在某些方面优于UV MALDI模式。这些结果可能为自上而下的蛋白质组学和蛋白质生物标志物鉴定提供突破。无基质激光解吸以及有效的大鼠脑组织和细菌的二维分析也被证明。基于该技术在第一阶段的能力证明，该仪器将应用于生物组织的分析。矩阵辅助和无矩阵方法都将被使用。仪器将进行升级，以进一步提高通量、灵敏度和分辨率。作为这项研究的一部分，Ionwerks正在与路易斯安那州立大学的Kermit Murray教授的质谱研究小组合作，后者是使用红外激光器直接分析生物分子的专家。将演示IR - LDI - IM - MS系统对滥用药物、代谢物、脂质和神经肽在大鼠脑组织切片上和大鼠模型内的空间成像的效用。一种先进的分析复杂生物系统的技术将被开发出来，与传统的紫外MALDI相比，它将具有显著的优势。这项技术的重要商业应用是生物医学研究和临床诊断。