Biomarker Quantitation by NP-MS Tissue Imaging.

描述（由申请人提供）：当通过纳米颗粒（NP）基质植入实现时，MALDI组织成像正在成熟为我们实验室和NIDA-IRP中生物标志物和药物发现的有用临床前工具。连续冠状切片的定量可重复图像表明，在啮齿动物疾病和损伤模型中，相对定量现在是可能的。纳米颗粒（NP）基质植入允许啮齿动物疾病和损伤模型中生物标志物水平差异的数值定量。进行组织的质谱分子成像的商业仪器没有能够分析和显示这些质量数据集的软件。1)软件和硬件开发下，我们的先行阶段II的多维数据分析，从我们的MALDI离子迁移率-MS成像系统将扩大，以满足这一需求，也将有双重用途，在相关的分子图像质量与纳米级表征的NP矩阵先进的材料分析技术，如亚纳米分辨率蔡司猎户座氦离子显微镜。2)独特的核壳NP基质的持续增长将继续得到植入组织后NP结构和粒度分布的先进材料分析测量的帮助。3)将标准品掺入组织表面的方案将试图证明MALDI-MS成像中相对和绝对定量的能力。

DESCRIPTION (provided by applicant): MALDI tissue imaging, when enabled by Nanoparticulate (NP) Matrix implantation, is maturing into a useful preclinical tool for biomarker and drug discovery in our laboratory and at NIDA-IRP. Quantitatively reproducible images from serial coronal sections suggest that relative quantitation is now possible in rodent models of disease and injury. Nanoparticulate (NP) matrix implantation allows numerical quantitation of differences in biomarker levels in rodent disease and injury models. No commercial instrument which performs mass spectral molecular imaging of tissues has software capable of analyzing and displaying these quality data sets. 1) Software and hardware developed under our antecedent phase II for analyzing the multidimensional data from our MALDI-Ion Mobility-MS imaging system will be expanded to serve this need and will also have dual use in correlating molecular image quality with the nano-scale characterization of the NP matrices by advance materials analysis techniques such as the subnanometer resolution Zeiss Orion Helium Ion Microscope. 2) The continued growth of unique core shell NP matrices will continue to be aided by advanced materials analysis measurement of the NP structures and particle size distributions after implantation into the tissue. 3) Protocols for standards incorporation into and onto the tissue surface will attempt to prove the capability of relative and absolute quantitation in MALDI-MS imaging.