Microsphere Coatings for Protein Arrays

用于蛋白质阵列的微球涂层

• 批准号: 7160473
• 负责人: Patrick E Guire
• 金额: $ 9.99万
• 依托单位: INNOVATIVE SURFACE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2009-04-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7160473
• 关键词: 3-Dimensional; Address; Adhesions; Binding; Binding Sites; Biological Assay; Biosensor; Capsid Proteins; Cell Adhesion; Characteristics; Chemicals; Class; Corrosion; Cross-Linking Reagents; Development; Devices; Diagnostic; Disease Management; Drug Interactions; Electronics; Film; Generations; Genetic; Genetic Predisposition to Disease; Genetic screening method; Genomics; Goals; Growth; Immobilization; Individual; Industry; Laboratories; Marketing; Medical; Medical Device; Microscope; Microspheres; Modification; Numbers; Object Attachment; Optics; Parents; Pattern; Phase; Polymers; Preparation; Printing; Procedures; Production; Property; Protein Array; Proteomics; Public Health; Readiness; Reading; Reagent; Reproducibility; Research Personnel; Sampling; Sea; Site; Slide; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solubility; Specificity; Surface; Surface Properties; Suspension substance; Suspensions; System; Technology; Testing; Texture; Water; Wettability; Work; base; chemical bond; cost; density; design; desire; high throughput screening; improved; nano; nanoparticle; particle; protein protein interaction; scale up; size; virtual

DESCRIPTION (provided by applicant): This SBIR project is designed to develop a versatile and cost-effective coating technology for surfaces used in microarray devices, especially for proteomic and genomic analyses. The "virtual well" strip, slide or plate array will be developed with new multifunctional photoactivatible crosslinking reagents for covalent thin film immobilization of micro- and nano-particles. The hydrophilic microparticles will form immobilized biomolecule assay sites and the hydrophobic nanoparticles will form "superhydrophobic" nanotextured surface separating the "virtual well" assay sites. The Phase II work is expected to develop a dense array capability utilizing self-encoded microparticles to provide multianalyte assay capability in each site or "address" of a microarray, with confidence of at least one million analyte capability on microscope slide array. Specific aims of this Phase I proposal include: 1) reproducible and cost-effective synthesis of a new class of oligofunctional photoreactive crosslinking reagents and their utility in immobilizing microparticles with covalent thin film generation on array support surfaces; 2) preparation of "virtual well" arrays via patterned immobilization of hydrophilic microparticles in assay sites separated by "superhydrophobic" nanotextured surfaces; and 3) demonstrate superior characteristics of proteomic arrays formed with immobilized microparticles vs flat surfaces. This proposed work is expected to generate new microarray coating technology which will provide significant improvements in analyte capacity (number) per chip, analyte assay sensitivity, and ease of sample application. The Phase I project will produce a new class of photoreactive film-forming reagents useful for coating a large variety of diagnostic and implantable medical devices, including the generation of superhydrophobic nanotextured surfaces for a variety of medical, diagnostic and electronic industry applications. The benefit of this proposed project to public health would be less expensive and more accurate microarrays for genetic and proteomic analysis. As genetic testing increases and more specific genetic predispositions to drug interactions are understood, the testing will need to be cheaper and more reliable. Additionally, the improved microarrays will benefit researchers studying protein-protein interactions which may ultimately greatly aid in disease management.

描述（由申请人提供）：该SBIR项目旨在为微阵列设备中使用的表面开发一种通用且具有成本效益的涂层技术，特别是用于蛋白质组学和基因组学分析。“虚拟井”条，载玻片或板阵列将开发与新的多功能光活化交联剂共价薄膜固定的微米和纳米粒子。亲水性微粒将形成固定的生物分子测定位点，并且疏水性纳米颗粒将形成分离“虚拟孔”测定位点的“超疏水”纳米纹理化表面。第二阶段的工作预计将开发一个密集的阵列能力，利用自我编码的微粒，以提供多分析物测定能力，在每个网站或“地址”的微阵列，至少有一百万分析物的能力，显微镜载玻片阵列的信心。 该阶段I提案的具体目标包括：1）一类新的寡官能光反应性交联试剂的可再现且成本有效的合成，以及它们在阵列支撑表面上通过共价薄膜生成固定微粒中的效用; 2）通过在由“超疏水”纳米纹理化表面隔开的测定位点中图案化固定亲水性微粒来制备“虚拟井”阵列;和3）证明了与平坦表面相比，用固定化微粒形成的蛋白质组阵列的上级特征。 这项拟议的工作预计将产生新的微阵列涂层技术，这将提供显着改善分析物容量（数量）每个芯片，分析物检测灵敏度，并易于样品的应用。第一阶段项目将生产一种新型的光反应成膜试剂，可用于涂覆各种诊断和植入式医疗器械，包括为各种医疗，诊断和电子工业应用产生超疏水纳米纹理表面。这项拟议项目对公共卫生的好处将是更便宜和更准确的基因和蛋白质组分析微阵列。随着基因检测的增加和对药物相互作用更具体的遗传倾向的了解，检测将需要更便宜和更可靠。此外，改进的微阵列将使研究蛋白质-蛋白质相互作用的研究人员受益，这最终可能大大有助于疾病管理。