A Novel Combination of Thermoresponsive and Nanofibrillar Surface for Cell Cultur

用于细胞培养的热响应和纳米纤丝表面的新型组合

• 批准号: 7394730
• 负责人: Patrick E Guire
• 金额: $ 9.97万
• 依托单位: INNOVATIVE SURFACE TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-25 至 2008-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7394730
• 关键词: Address; Affect; Biological Assay; Biomimetics; Cell Adhesion; Cell Culture Techniques; Cell Differentiation process; Cell Line; Cell Surface Receptors; Cell Survival; Cell Therapy; Cell physiology; Cell surface; Cells; Cellular Morphology; Clinical Research; Condition; Cultured Cells; Data; Digestion; Disease; Environment; Enzymes; Evaluation; Extracellular Matrix; Gap Junctions; Gene Expression; Generations; Goals; Government; Growth; Harvest; Hour; Hydration status; Individual; Invasive; Mammalian Cell; Marketing; Mechanics; Persons; Phase; Phase I Clinical Trials; Polymers; Porosity; Process; Property; Purpose; Rate; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solutions; Supporting Cell; Surface; System; Technology; Temperature; Texture; Time; Tissue Engineering; Tissues; Toxic effect; Trypsin; base; cell growth; cell type; cold temperature; design; improved; in vivo; innovation; nanofiber; novel; poly-N-isopropylacrylamide; stem; surface coating

DESCRIPTION (provided by applicant): This SBIR Phase I study is designed to develop and provide to the cell culture market, significantly improved surfaces for cell growth, differentiation control, and harvesting. The combination of two recently demonstrated improvements (thermally-responsive polymer for facile and gentle cell/tissue release from the support surface and nanofibrillar texture for improved cell adhesion and native property expression) will be demonstrated and compared with current commercial cell culture surfaces. The nanofibers for this textured surface will be electrospun or photochemically coated with thermoresponsive polymer. Thin coating or spinning the nanofibers with thermoresponsive polymer will allow the culture of stem and other mammalian cells in a more native tissue-like environment and allow rapid detachment of cells and cell sheets without the damage from trypsin or mechanical scraping release treatment. Thus, this proposed new cell culture surface technology offers a temperature-responsive biomimetic surface with potential applications in tissue engineering, cell therapy, cellomic (cell arrays for toxicity assays) and cell-matrix interaction studies.Project Narrative This project will develop surfaces designed for the culture and harvest of mammalian cells in a more defined, disease-free and native tissue-like growth environment. This nanotextured surface with temperature- responsive release polymer will enable the growth and controlled differentiation of cells as individuals or thin tissues and their release from the growth surface without damage from digestive enzymes or mechanical scraping. This significant improvement in cell culture technique will add great value to basic and clinical research leading to cell therapy where a ready supply of selected and healthy cells are needed.

描述(由申请人提供)：这项SBIR第一阶段研究旨在开发并向细胞培养市场提供显著改善的表面，用于细胞生长、分化控制和收获。将展示最近展示的两种改进的组合(用于轻松和温和地从支撑表面释放细胞/组织的热响应聚合物和用于改善细胞粘附性和原生性表达的纳米纤维织构)，并与目前的商业细胞培养表面进行比较。这种纹理表面的纳米纤维将被电纺或光化学涂覆热响应性聚合物。用热响应性聚合物对纳米纤维进行薄涂层或纺丝，将允许在更自然的组织状环境中培养干细胞和其他哺乳动物细胞，并允许细胞和细胞膜快速分离，而不会受到胰酶或机械刮除释放处理的损害。因此，这项拟议的新细胞培养表面技术提供了一种温度响应型仿生表面，在组织工程、细胞治疗、细胞学(用于毒性分析的细胞阵列)和细胞-基质相互作用研究中具有潜在的应用。项目简介该项目将开发设计用于在更明确、无疾病和自然组织样生长环境中培养和收获哺乳动物细胞的表面。这种带有温度响应性释放聚合物的纳米结构表面将使细胞作为个体或薄组织的生长和受控分化以及它们从生长表面释放，而不会受到消化酶或机械刮擦的损害。细胞培养技术的这一重大改进将为基础和临床研究增加巨大价值，从而导致需要随时供应精选和健康细胞的细胞治疗。