A Novel Combination of Thermoresponsive and Nanofibrillar Surface for Cell Cultur

描述（由申请人提供）：本SBIR I期研究旨在开发并向细胞培养市场提供显著改善的细胞生长、分化控制和收获表面。将证明两种最近证明的改进的组合（用于从支持表面容易且温和地释放细胞/组织的热响应聚合物和用于改善细胞粘附和天然性质表达的纳米原纤纹理），并与当前商业细胞培养表面进行比较。用于这种纹理化表面的纳米纤维将被电纺或光化学涂覆有热敏聚合物。用热敏聚合物薄涂覆或纺制纳米纤维将允许在更天然的组织样环境中培养干细胞和其他哺乳动物细胞，并允许细胞和细胞片的快速分离，而不会受到胰蛋白酶或机械刮擦释放处理的损害。因此，这一建议的新的细胞培养表面技术提供了一个温度响应的仿生表面与潜在的应用在组织工程，细胞治疗，细胞组学（细胞阵列的毒性测定）和细胞-基质相互作用的研究。项目叙述本项目将开发设计用于培养和收获哺乳动物细胞在一个更明确的，无疾病和天然组织样生长环境的表面。这种具有温度响应性释放聚合物的纳米纹理表面将使得细胞能够作为个体或薄组织生长和受控分化，并且它们从生长表面释放，而不会受到消化酶或机械刮擦的损害。细胞培养技术的这一重大改进将为基础和临床研究增加巨大价值，从而导致细胞治疗，其中需要现成的选择和健康细胞供应。

DESCRIPTION (provided by applicant): This SBIR Phase I study is designed to develop and provide to the cell culture market, significantly improved surfaces for cell growth, differentiation control, and harvesting. The combination of two recently demonstrated improvements (thermally-responsive polymer for facile and gentle cell/tissue release from the support surface and nanofibrillar texture for improved cell adhesion and native property expression) will be demonstrated and compared with current commercial cell culture surfaces. The nanofibers for this textured surface will be electrospun or photochemically coated with thermoresponsive polymer. Thin coating or spinning the nanofibers with thermoresponsive polymer will allow the culture of stem and other mammalian cells in a more native tissue-like environment and allow rapid detachment of cells and cell sheets without the damage from trypsin or mechanical scraping release treatment. Thus, this proposed new cell culture surface technology offers a temperature-responsive biomimetic surface with potential applications in tissue engineering, cell therapy, cellomic (cell arrays for toxicity assays) and cell-matrix interaction studies.Project Narrative This project will develop surfaces designed for the culture and harvest of mammalian cells in a more defined, disease-free and native tissue-like growth environment. This nanotextured surface with temperature- responsive release polymer will enable the growth and controlled differentiation of cells as individuals or thin tissues and their release from the growth surface without damage from digestive enzymes or mechanical scraping. This significant improvement in cell culture technique will add great value to basic and clinical research leading to cell therapy where a ready supply of selected and healthy cells are needed.