Proteomics

蛋白质组学核心设施提供了蛋白质化学、分离和质量的交互资源 光谱分析。蛋白质组学核心设施为基础科学的研究需求提供具体的 有针对性的功能研究，包括鉴定相互作用的蛋白质 免疫共沉淀，翻译后修饰的定位，以及检测方法的发展 酶活性。对于临床和翻译项目，蛋白质组学核心设施提供图谱分析 服务，使用二维凝胶电泳或液相色谱与质谱联用，以及 数据挖掘指示的相关蛋白质的后续鉴定。此外，社区教育是一种 在莫菲特癌症中心开发这一新设施的关键要素；培训通过一系列 研讨会和资料可在内部网站上查阅。

The Proteomics Core Facility provides an interactive resource for protein chemistry, separations, and mass spectrometry. The Proteomics Core Facility serves the research needs of basic science with specifically targeted functional investigations, including the identification of interacting proteins isolated by coimmunoprecipitation, localization of post-translational modifications, and development of assays for enzymatic activity. For clinical and translational projects, the Proteomics Core Facility offers profiling services, using either 2D gel electrophoresis or liquid chromatography coupled to mass spectrometry, and subsequent identification of relevant proteins indicated by data mining. In addition, community education is a key element of developing this new facility at Moffitt Cancer Center; training is provided through a series of seminars and resources are made available on an internal Web site.