Molecular Biology

分子生物学

• 批准号: 7516319
• 负责人: William Steven Dalton
• 金额: $ 10.57万
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7516319
• 关键词: Capillary Electrophoresis; Computer software; Consensus Sequence; Consultations; Cosmids; Custom; DNA; DNA Sequence; DNA analysis; Data; Data Storage and Retrieval; Databases; Detection; Development; Discrimination; Equipment; Gene Expression; Genotype; Learning; Molecular Biology; Molecular Profiling; Plasmids; Polymerase Chain Reaction; Preparation; Quality Control; Research Personnel; Running; Sampling; Sequence Analysis; Services; Standards of Weights and Measures; System; Testing; Training; bisulfite; design

The Molecular Biology Core offers automated, quality-controlled DNA sequencing, qPCR for gene expression or SNP analysis, as well as fragment analysis using a capillary electrophoresis system, and highthroughput genotyping using Beckman Coulter's SNPstream system. Researchers may choose to have the Core handle entire projects from sample to finished results for gene expression, or use one of the 7900HT Sequence Detection Systems for running self-user plates. In addition the Molecular Biology Core offers training in equipment and software usage for qPCR and fragment analysis for self-users. Consultation is available for all services, including primer design, sample prep, data interpretation and analysis. Services provided: 1. Sequence analysis of plasmid, BAG, and cosmid DNA, PCR products, bisulfite treated DNA 2. Gene expression profiles with standard or custom primer-probe sets 3. Genotyping - a) SNPstream high throughput system; b) TaqMan Allelic Discrimination; c) Fragment analysis 4. Additional services - a) Primer design for custom templates and qPCR primer-probe sets; b) Sample preparation information for optimal results; c) Sample testing prior to sequencing for quality; d) Results interpretation assistance; e) Contig assembly for consensus sequences; f) Homology and database searches; g) Permanent data storage; h) Large project sequencing involving design and delivery of a complete sequence; I) qPCR data supplied as Excel spreadsheets; j) Project development for the SNPstream system 5. Training - a) Training for unassisted use of qPCR to insure optimal results; b) Assistance in learning and using the DNA analysis software, DNASTAR; c) Fragment analysis training

分子生物学核心提供自动化，质量控制的DNA测序，qPCR基因 表达或SNP分析，以及使用毛细管电泳系统的片段分析，以及高通量 使用贝克曼库尔特的SNPstream系统进行基因分型。研究人员可能会选择 核心处理整个项目从样品到完成的基因表达结果，或使用7900 HT之一 序列检测系统运行的自我用户板。此外，分子生物学核心提供 为自我用户提供qPCR和片段分析的设备和软件使用培训。协商是 可用于所有服务，包括引物设计、样品制备、数据解释和分析。 提供的服务： 1.质粒、BAG和粘粒DNA、PCR产物、亚硫酸氢盐处理的DNA的序列分析 2.使用标准或定制引物-探针组的基因表达谱 3.基因分型- a）SNPstream高通量系统; B）TaqMan等位基因鉴别; c）片段 分析 4.附加服务- a）定制模板和qPCR引物-探针组的引物设计; B）样品 c）测序前的样品质量检测; d） 结果解释辅助; e）共有序列的重叠群组装; f）同源性和 数据库搜索; g）永久数据存储; h）涉及设计和 完整序列的交付; I）以Excel电子表格形式提供的qPCR数据; j）项目 SNPstream系统的开发 5.培训- a）培训qPCR的无辅助使用，以确保获得最佳结果; B）协助学习 c）片段分析培训