DEVELOPMENTAL NEUROTOXICOLOGY PROGRAM

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在2005年期间，发育神经毒理学计划继续专注于开发与复杂反应时间行为测试结合使用的正电子发射断层扫描（PET）程序。在与灵长类动物中心PET成像服务中心主任Satoshi Minoshima博士的合作下，我们已经完成了产前暴露于甲基汞的成年食蟹猴功能性PET成像的培训程序。对反应时间测试范例和测试设备进行了变更，以适应功能成像的独特要求。已经实现了盐水注射的中试运行。2005年成功举办了六次PET培训班。目前正在审查这些PET会议的大脑图像。实验室中的三个人，包括汤姆·伯巴赫博士，都获得了安全处理放射性材料的认证。Drs. Burbacher和Grant还在继续努力调整发育神经毒理学项目中使用的操作性试验程序，以用于年幼动物。为此，正在训练3个月至1.5岁的猕猴自愿进入测试椅，触摸并握住反应时间测试面板上的主栏（反应时间测试的必要先决条件）。我们使用的保持时间从0.5秒到5秒不等。虽然大多数动物都可以成功地训练接触主栏的短暂保持时间（最多3秒），但更长的保持时间已被证明是更困难的。使用较短的触摸和保持时间，已经从动物的子集获得了稳定的反应时间和运动时间。2006年将采用反应时间滴定法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During 2005, the Developmental Neurotoxicology Program has continued concentrating on the development of the Positron Emission Tomography (PET) procedures to be used in conjunction with complex reaction time behavioral testing. In collaboration with Dr. Satoshi Minoshima, Director of the PET imaging service core at the Primate Center, we have completed training procedures for functional PET imaging in adult Macaca fascicularis prenatally exposed to methylmercury. Changes were made to the reaction time test paradigm and test equipment to accommodate the unique requirements of functional imaging. Pilot runs with saline injections have been achieved. Six successful PET sessions were accomplished in 2005. The brain images from these PET sessions are currently being reviewed. Three individuals in the laboratory, including Dr. Tom Burbacher, are certified in the safe handling of radioactive materials. There are also continued efforts by Drs. Burbacher and Grant to adapt the operant test procedures used in the Developmental Neurotoxicology Program for use with younger animals. To this end, macaque monkeys ranging from 3 months to 1.5 years are being trained to voluntarily enter the testing chair and touch and hold the home bar on the reaction time test panel (a necessary prerequisite for reaction time testing). We are using hold times that vary from .5 seconds to 5 seconds. While most animals can successfully be trained to touch the home bar for brief hold times (up to 3 seconds), longer hold times have proven to be more difficult. Using the shorter touch and hold times, stable reaction times and movement times have been obtained from a subset of animals. Titration of reaction times will be introduced in 2006.