MULTIPLEX NHC TYPING OF RHESUS MACAQUES

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The main goal of this small research grant was to develop a new research technology for the rapid identification of major histocompatibility complex class I (MHC-I) alleles present in the genome of rhesus macaques (macaca mulatta). MHC-I genes encode proteins central to the adaptive immune response, signaling infection by binding pathogenic peptides and presenting them on the cell surface to cytotoxic t cells. In addition, class I molecules are critical to the innate immune response, providing both inhibitory and stimulating signals to NK cells. Rhesus macaques are relevant preclinical models for human diseases and transplantation, and experimentally infected rhesus monkeys serve as an indispensable animal model to assess the pathogenesis, to validate therapy approaches and to develop vaccination strategies against AIDS. As in humans, the disease course in macaques is variable, and certain MHC-I molecules appear to be associated with better control SIV replication. Current molecular methods for genotyping rhesus monkeys use PCR techniques with individual sets of sequence-specific primers for a handful of Mamu-A and -B alleles. In order to develop a DNA assay that can simultaneously detect a large number of Mamu-A and -B alleles, we will combine the multiplexing capacity of the Luminex 100 platform with the high specificity of sequence-specific DNA probes (SSP) that contain minor groove binders (MGBs). MGBs allow the design of short probes with increase sensitivity to differentiate single nucleotide polymorphism (SNP), whereas the Luminex microspheres permit the simultaneous use of up to 100 different probes.