IMMUNOGENICITY OF HSV AMPLICONS IN RHESUS MACAQUES

HSV 扩增子在恒河猴中的免疫原性

• 批准号: 7562431
• 负责人: Luis David Giavedoni
• 金额: $ 9.7万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562431
• 关键词: Antigens; CD8B1 gene; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Genome; Grant; HIV; Immune response; Institution; Intramuscular; Longitudinal Studies; Macaca mulatta; Mucosal Immunity; Natural Killer Cells; Prevention strategy; Production; Proteins; Research; Research Personnel; Resources; Route; SIV; Serum; Simplexvirus; Source; Staining method; Stains; T-Lymphocyte; United States National Institutes of Health; Vaccines; Virus Diseases; cytokine; immunogenicity; lymphocyte proliferation; novel; response; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The best strategy for prevention of human immunodeficiency virus (HIV) infection involves the development of an effective vaccine. Herpes simplex virus (HSV) amplicons represent a novel and promising strategy for vaccine development. The studies described in this proposal will determine if HSV amplicons can elicit a significant immune response in the rhesus macaque. Specifically, rhesus macaques will be inoculated via an intramuscular route with HSV amplicons that express a replication-defective SIvmax239 genome or by control amplicons that do not express any SIV gene products. The cellular immune response to SIV proteins will be followed through analysis of CD4+ and CD8+ T cell responses, tetramer staining, and antigen-specific cytokine production. The humoral response will be determined by analysis of both serum and mucosal immunity. Finally, the innate immune response will be determined through analysis of both natural killer cells and by performing longitudinal studies of changes in lymphocyte proliferation and activation.

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