IMMUNOGENICITY OF HSV AMPLICONS IN RHESUS MACAQUES

HSV 扩增子在恒河猴中的免疫原性

• 批准号: 7562431
• 负责人: Luis David Giavedoni
• 金额: $ 9.7万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7562431
• 关键词: Antigens; CD8B1 gene; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Genome; Grant; HIV; Immune response; Institution; Intramuscular; Longitudinal Studies; Macaca mulatta; Mucosal Immunity; Natural Killer Cells; Prevention strategy; Production; Proteins; Research; Research Personnel; Resources; Route; SIV; Serum; Simplexvirus; Source; Staining method; Stains; T-Lymphocyte; United States National Institutes of Health; Vaccines; Virus Diseases; cytokine; immunogenicity; lymphocyte proliferation; novel; response; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The best strategy for prevention of human immunodeficiency virus (HIV) infection involves the development of an effective vaccine. Herpes simplex virus (HSV) amplicons represent a novel and promising strategy for vaccine development. The studies described in this proposal will determine if HSV amplicons can elicit a significant immune response in the rhesus macaque. Specifically, rhesus macaques will be inoculated via an intramuscular route with HSV amplicons that express a replication-defective SIvmax239 genome or by control amplicons that do not express any SIV gene products. The cellular immune response to SIV proteins will be followed through analysis of CD4+ and CD8+ T cell responses, tetramer staining, and antigen-specific cytokine production. The humoral response will be determined by analysis of both serum and mucosal immunity. Finally, the innate immune response will be determined through analysis of both natural killer cells and by performing longitudinal studies of changes in lymphocyte proliferation and activation.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 预防人类免疫缺陷病毒（HIV）感染的最佳策略是研制有效的疫苗。 单纯疱疹病毒（HSV）扩增子代表了一种新的和有前途的疫苗开发策略。 本提案中描述的研究将确定HSV扩增子是否可以在恒河猴中引起显著的免疫应答。 具体而言，恒河猴将通过肌内途径接种表达复制缺陷型SIvmax 239基因组的HSV扩增子或不表达任何SIV基因产物的对照扩增子。 将通过分析CD 4+和CD 8 + T细胞应答、四聚体染色和抗原特异性细胞因子产生来跟踪对SIV蛋白的细胞免疫应答。 将通过分析血清和粘膜免疫来确定体液应答。 最后，通过分析自然杀伤细胞和进行淋巴细胞增殖和活化变化的纵向研究来确定先天免疫应答。