LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
基本信息
- 批准号:8357650
- 负责人:
- 金额:$ 13.85万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-05-01 至 2012-04-30
- 项目状态:已结题
- 来源:
- 关键词:AgingAnimal ModelAnimalsAntibodiesCallithrixCebidaeCellsCercopithecidaeCercopithecus pygerythrusComplexFamily suidaeFlow CytometryFundingGeneticGrantHumanImmune systemImmunologicsInflammationMacacaMacaca mulattaMediator of activation proteinModelingMonoclonal AntibodiesNational Center for Research ResourcesObesityPan GenusPapioPathogenesisPhysiologicalPhysiologyPlayPrimatesPrincipal InvestigatorProtocols documentationReagentRecombinant AntibodyRecoveryResearchResearch InfrastructureResourcesRoleScientistSignal TransductionSiteSourceSystemTailTechnologyTestingTubeUnited States National Institutes of Healthbasechemokinecostcytokineimprovednonhuman primatenovelpre-clinical researchvaccine developmentweb site
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Nonhuman primates (NHP) are the preferred animal models for pre-clinical research because they approximate humans in physiology and genetics more closely than any other animal. Critical advances in immunologic research have been made through the use of the NHP model, most notably in aids pathogenesis, treatment, and vaccine development. Cytokines and chemokines are soluble mediators of the immune system that play a crucial role in intercellular signaling, and in the recruitment of cells to inflammation sites. Identification of these molecules in NHP is crucial for the understanding of complex physiological and pathological mechanisms that occur in these species, and to demonstrate whether these mechanisms function similarly in humans. The luminex100 system is a bench-top flow cytometer that allows the user to quantify up to 100 molecules simultaneously in a single tube. Recently, we identified several antibodies specific for human cytokines that have the capacity to recognize homologous chemokines and cytokines of NHP origin. Currently, we have a panel of reagents that allow for the simultaneous identification of 23 cytokines and chemokines from chimpanzees and the old world monkeys rhesus macaques, baboons, cynomolgus macaques, pig-tailed macaques, and African green monkeys. in this r-24 application we propose to facilitate and expand the use of the Luminex technology in NHP by completing the following specific aims: 1) to improve the breadth and analytical power of our 23-plex NHP Luminex panel by increasing the number of detectable molecules in old world monkey species; 2) to improve the quality and depth of our 23-plex NHP Luminex panel by increasing the sensitivity of some suboptimal reagents currently used for quantification of cytokines from old world monkey species; and 3) to develop recombinant antibodies for the simultaneous quantification of cytokines in the marmoset, a new world monkey specie increasingly used in vaccine development, aging, and obesity research. We will perform extensive testing of available monoclonal antibodies against old world monkey cytokines, and state-of-the-art recombinant antibody technology for the creation of antibodies against marmoset cytokines. The information and protocols generated from these studies will be listed in the flow cytometry website of the SNPRC; novel antibodies will be distributed to scientists who justify their need on cost-recovery bases.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Luis David Giavedoni其他文献
Luis David Giavedoni的其他文献
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{{ truncateString('Luis David Giavedoni', 18)}}的其他基金
Development of Immunological Reagents for the Identification of New World Monkey Biomarkers
开发用于鉴定新世界猴生物标志物的免疫试剂
- 批准号:
10807651 - 财政年份:2023
- 资助金额:
$ 13.85万 - 项目类别:
Development of Immunological Reagents for the Identification of New World Monkey Biomarkers
开发用于鉴定新世界猴生物标志物的免疫试剂
- 批准号:
10592259 - 财政年份:2022
- 资助金额:
$ 13.85万 - 项目类别:
Development of Immunological Reagents for the Identification of New World Monkey Biomarkers
开发用于鉴定新世界猴生物标志物的免疫试剂
- 批准号:
10334632 - 财政年份:2022
- 资助金额:
$ 13.85万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
8172652 - 财政年份:2010
- 资助金额:
$ 13.85万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
7957900 - 财政年份:2009
- 资助金额:
$ 13.85万 - 项目类别:
LUMINEX TECHNOLOGY FOR THE QUANTIFICATION OF CYTOKINES IN NON-HUMAN PRIMATES
用于非人类灵长类动物细胞因子定量的 LUMINEX 技术
- 批准号:
7716084 - 财政年份:2008
- 资助金额:
$ 13.85万 - 项目类别:
IMMUNOGENICITY OF HSV AMPLICONS IN RHESUS MACAQUES
HSV 扩增子在恒河猴中的免疫原性
- 批准号:
7562431 - 财政年份:2007
- 资助金额:
$ 13.85万 - 项目类别:
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