ESTABLISHMENT AND EXPANSION OF A SPF RHESUS COLONY: AIDS, ANIMAL MODEL

SPF 恒河猴群体的建立和扩展：艾滋病、动物模型

• 批准号: 8359525
• 负责人: RUDOLF Paul BOHM
• 金额: $ 41.11万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359525
• 关键词: Acquired Immunodeficiency Syndrome; Address; Animal Model; Animals; Behavioral; Breeding; Censuses; Disease; Funding; Future; Gastrointestinal Agents; Gastrointestinal Diseases; Genetic Variation; Grant; Investigation; Macaca mulatta; Morbidity - disease rate; National Center for Research Resources; Opportunistic Infections; Population; Prevention; Primates; Production; Research; Research Personnel; Research Project Grants; Role; Screening procedure; Source; Specific Pathogen Frees; United States National Institutes of Health; Viral; biomedical resource; gene therapy; germ free condition; meetings; mortality; offspring; programs; social group; treatment strategy; vaccine development

DESCRIPTION (provided by applicant): Over the past five years the Tulane National Primate Research Center (TNPRC) has successfully developed and expanded its specific pathogen free (SPF) rhesus monkey breeding colony. By the end of the current funding period the U24 SPF colony will have grown from a population of zero animals in Year 1 to greater than 1000 animals at the end of Year 5. The colony was completely derived from the TNPRC conventional colony and has matured to the point that production over the next five years will create a self sustaining population allowing assignment of approximately 150 animals per year to NIH funded AIDS research programs. The entire TNPRC SPF colony supported by U24 and U42 grants is the largest in the NCRR/OAR funded program comprising 36 percent of the total animals reflected in the current program census. Over half of the animal allocations from the TNPRC SPF colony over the past five years have been to affiliate (outside) investigators demonstrating our commitment as a national biomedical resource. The further characterization and expansion of the SPF colony proposed in this application will be accomplished using several strategies. Funds have been requested to provide support for the founder SPF breeding stock and their offspring until assignment to AIDS research projects occurs. Funds for viral screening of animals have been requested. The expanded SPF9 (super clean) colony will continue to be grown to meet the needs of specialized AIDS studies including gene therapy, vaccine development and the investigation of opportunistic infections. Because gastrointestinal disease is the leading cause of morbidity and mortality in the TNPRC SPF breeding colonies, we plan to screen animals for selected gastrointestinal agents to further clarify their roles in disease and develop prevention and possible treatment strategies. Behavioral studies will be performed to address specific issues related to the formation of social groups of unrelated animals which has been required to maintain genetic diversity and maintain SPF status. For the colony that is supported by this grant, it is anticipated that 1000 rhesus monkeys will be maintained into the future. The size of the entire TNPRC SPF colony (support from all sources) will be over 3200 animals. Continued support of the TNPRC SPF colony will assist in alleviating the critical national shortage of well characterized rhesus monkeys for the AIDS research effort.

描述（由申请人提供）：在过去五年中，杜兰国家灵长类动物研究中心 (TNPRC) 成功开发并扩大了其无特定病原体 (SPF) 恒河猴繁殖群。到当前资助期结束时，U24 SPF 群体将从第一年的零动物数量增长到第五年末的超过 1000 只动物。该群体完全源自 TNPRC 传统群体，并且已经成熟到未来五年的生产将创建一个自我维持的群体，允许每年将大约 150 只动物分配给 NIH 资助的艾滋病研究项目。由 U24 和 U42 拨款支持的整个 TNPRC SPF 群体是 NCRR/OAR 资助计划中最大的群体，占当前计划普查中反映的动物总数的 36%。过去五年中，TNPRC SPF 群体分配的动物中有一半以上分配给了附属（外部）研究人员，这证明了我们作为国家生物医学资源的承诺。 本申请中提出的 SPF 菌落的进一步表征和扩展将使用多种策略来完成。已请求资金为创始人 SPF 种畜及其后代提供支持，直至分配到艾滋病研究项目为止。已请求资金用于动物病毒筛查。扩大后的SPF9（超洁净）菌落将继续生长，以满足专门的艾滋病研究的需要，包括基因治疗、疫苗开发和机会性感染的调查。由于胃肠道疾病是 TNPRC SPF 繁殖群体发病和死亡的主要原因，因此我们计划筛选动物中选定的胃肠道药物，以进一步阐明其在疾病中的作用，并制定预防和可能的治疗策略。将进行行为研究，以解决与无关动物形成社会群体相关的具体问题，这是维持遗传多样性和维持 SPF 状态所必需的。 这笔赠款支持的猴群预计未来将饲养 1000 只恒河猴。整个 TNPRC SPF 群体的规模（来自所有来源的支持）将超过 3200 只动物。 TNPRC SPF 群体的持续支持将有助于缓解全国用于艾滋病研究工作的特征良好的恒河猴的严重短缺问题。