INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY

减数分裂纺锤体蛋白质之间的相互作用

• 批准号: 7420716
• 负责人: Aaron M Neiman
• 金额: $ 1.72万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420716
• 关键词: electron microscopy; meiosis; microtubules; proteins; spindle pole body; spores

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During meiosis, the outer plaque of the spindle pole body (SPB) is reorganized.The structure is converted from a site of microtubule growth to one of membrane formation and anchorage. These membranes, termed prospore membranes, are essential for the formation of spores. Electron microscopy analysis reveals a very highly organized structure for the meiotic outer plaque, and two-hybrid analyses have identified many interactions between these outer plaque proteins. However, precisely how these proteins are organized with respect to each other within the outer plaque is not known. The arrangement of these proteins within the outer plaque will be explored by FRET-based methodology.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在减数分裂过程中，纺锤体极体(SPB)的外部斑块发生重组，结构由微管生长部位转变为膜形成和锚定部位。这些膜被称为前孢子膜，对于孢子的形成是必不可少的。电子显微镜分析显示减数分裂外斑具有高度组织化的结构，双杂交分析发现这些外斑蛋白之间存在许多相互作用。然而，这些蛋白质在外部斑块中是如何相互组织的还不清楚。这些蛋白质在外部斑块中的排列将通过基于FRET的方法进行探索。