Mechanisms of de novo membrane assembly

从头膜组装的机制

• 批准号: 10578067
• 负责人: Aaron M Neiman
• 金额: $ 31.36万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10578067
• 关键词: Adaptor Signaling Protein; Address; Autophagosome; Biochemical; Biological Models; Biological Process; Bypass; Cell physiology; Cells; Centrioles; Cytology; Cytoplasm; D Cells; Defect; Disease; Docking; Encapsulated; Endoplasmic Reticulum; Eukaryotic Cell; Event; Experimental Genetics; Fluorescence; Generations; Genetic; Guanine Nucleotides; Health; Human; Hydrophobicity; Intracellular Membranes; Lead; Licensing; Lipids; Mediating; Meiosis; Membrane; Membrane Fusion; Microtubule-Organizing Center; Modeling; Molecular; Mutation; Nature; Neurodegenerative Disorders; Organelles; Orthologous Gene; Pathologic; Pathway interactions; Point Mutation; Population; Process; Protein Family; Proteins; Reporting; Role; SNAP receptor; Saccharomycetales; Site; Surface; Syndrome; System; Techniques; Testing; Vesicle; Yeasts; cell behavior; ciliopathy; experimental study; flexibility; in vitro testing; in vivo; insight; interest; lipid transfer protein; membrane assembly; nervous system disorder; protein complex; protein purification; rab GTP-Binding Proteins; recruit; spindle pole body; target SNARE proteins; vesicular SNARE proteins

7. PROJECT SUMMARY/ABSTRACT The ability to rearrange vesicular traffic to create new membrane compartments de novo is a fundamental cell biological process important for many aspects of cellular physiology and differentiation. Two common examples of this phenomenon are the creation of autophagosomes and the generation of the ciliary sheath. In both cases, vesicles coalesce on a protein substrate to create a small double membrane that subsequently expands into a distinct organelle. Defects in these processes are associated with a variety of diseases, highlighting the importance of this aspect of cellular physiology to human health. Prospore membrane formation during the process of sporulation in budding yeast is another example of a de novo membrane formation event. Prospore membrane formation occurs on the microtubule organizing center of the cells, analogous to the ciliary sheath in higher cells, and the prospore membrane expands to encapsulate cytoplasmic components, analogous to an autophagosome. These similarities are due to parallels at the molecular level that make yeast sporulation a powerful model system to identify the underlying molecular mechanisms of membrane assembly. The experiments in this proposal are focused on two aspects of this process: 1) how the MOP promotes coalescence of vesicles to create the prospore membrane, and 2) how the Vps13 lipid transfer protein promotes membrane expansion.

7.项目总结/摘要 重新排列囊泡运输以从头产生新的膜隔室的能力是一种新的生物学机制。 基本的细胞生物学过程，对细胞生理学和分化的许多方面都很重要。两 这种现象的常见例子是自噬体的产生和纤毛的产生。 鞘在这两种情况下，囊泡在蛋白质底物上结合，形成一个小的双膜， 随后扩展成一个独特的细胞器。这些过程中的缺陷与各种 疾病，突出了细胞生理学的这一方面对人类健康的重要性。原孢子 芽殖酵母中孢子形成过程中的膜形成是另一个从头开始的例子。 膜形成事件。原孢子膜的形成发生在细胞壁微管组织中心， 细胞，类似于高等细胞中的纤毛鞘，原孢子膜扩张以包裹 细胞质成分，类似于自噬体。这些相似之处是由于 分子水平，使酵母孢子形成一个强大的模型系统，以确定潜在的分子 膜组装的机制。该提案中的实验集中在两个方面 过程：1）MOP如何促进囊泡聚结以产生原孢子膜，以及2）MOP如何促进囊泡聚结以产生原孢子膜。 vps 13脂质转运蛋白促进膜扩张。