Assembly and Function of the Yeast Spore Wall

酵母孢子壁的组装和功能

• 批准号: 6966008
• 负责人: Aaron M Neiman
• 金额: $ 26.63万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-12 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6966008
• 关键词: Saccharomyces cerevisiae; antibody; cell biology; cell cycle; developmental genetics; extracellular matrix; fungal genetics; fungal proteins; gene deletion mutation; genetic regulation; genetic screening; glucans; hexosyltransferase; immunoelectron microscopy; protein localization; protein protein interaction; protein structure function; scanning electron microscopy; spores; suppressor mutations; transmission electron microscopy

All cells surround themselves with an extracellular matrix that provides both physical support and protection for the cell. In multicellular organisms the extracellular matrices from individual cells combine to form much larger structures that provide the scaffolding for the body of the organism. It is important, therefore, to understand how extracellular matrices are constructed and connected. The formation of the spore wall, a stratified, multicellular extracellular matrix, in the yeast Saccharomyces cerevisiae provides an excellent model system to study the assembly of extracellular matrices. An initial characterization of a collection of mutants defective in spore wall formation has provided an outline of the pathway of spore wall assembly. This initial work will be expanded by further studies on the role of the different spore wall assembly genes in promoting formation of the wall. The localization and activity of the beta-glucan and chitin syntheses, which catalyze the formation of the major spore wall polysaccharides, will be investigated both in wild type cells and in cells mutant for putative regulatory genes. Additionally, the molecular mechanism of action of the AMA1 gene, which links the exit from meiosis to the onset of spore wall formation will be explored. Finally, the outer layers of the spore wall both allow the spore to resist environmental stress and connect individual spores together through bridges. The targeting and anchoring to the spore wall of proteins involved in the assembly of these layers will be investigated. Also, a collection of mutants with modest defects in outer spore wall assembly will be analyzed to define genes involved in acquisition of resistance to specific stresses. In sum, these studies should deepen our understanding of the molecular mechanisms of spore wall construction, which will in turn provide insight into the strategies of extracellular matrix assembly in other organisms.

所有细胞都被细胞外基质包围，细胞外基质为细胞提供物理支持和保护。在多细胞生物中，来自单个细胞的细胞外基质结合形成更大的结构，为生物体的身体提供支架。因此，了解细胞外基质是如何构建和连接的是很重要的。酵母孢子壁是一种分层的多细胞胞外基质，它的形成为研究胞外基质的组装提供了一个很好的模型系统。一组孢子壁形成缺陷突变体的初步表征提供了孢子壁组装途径的轮廓。这项初步工作将通过进一步研究不同孢子壁组装基因在促进孢子壁形成中的作用而得到扩展。催化主要孢子壁多糖形成的β -葡聚糖和几丁质合成的定位和活性将在野生型细胞和突变型细胞中进行研究，以确定可能的调控基因。此外，还将探讨AMA1基因的分子作用机制，该基因将减数分裂的退出与孢子壁形成的开始联系起来。最后，孢子壁的外层既可以使孢子抵抗环境压力，又可以通过桥梁将单个孢子连接在一起。将研究这些层组装过程中涉及的蛋白质在孢子壁上的靶向和锚定。此外，在孢子外壁组装中具有适度缺陷的突变体的集合将被分析，以确定参与获得对特定胁迫的抗性的基因。总之，这些研究将加深我们对孢子壁构建的分子机制的理解，这将反过来为其他生物的细胞外基质组装策略提供见解。