INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY

减数分裂纺锤体蛋白质之间的相互作用

• 批准号: 8365796
• 负责人: Aaron M Neiman
• 金额: $ 2.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365796
• 关键词: Biology; Electron Microscopy; Fluorescence Resonance Energy Transfer; Funding; Fungal Genome; Grant; Growth; Meiosis; Membrane; Methodology; Microtubules; National Center for Research Resources; Principal Investigator; Proteins; Reproduction spores; Research; Research Infrastructure; Resources; Site; Source; Structure; United States National Institutes of Health; base; cost; spindle pole body; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During meiosis, the outer plaque of the spindle pole body (SPB) is reorganized.The structure is converted from a site of microtubule growth to one of membrane formation and anchorage. These membranes, termed prospore membranes, are essential for the formation of spores. Electron microscopy analysis reveals a very highly organized structure for the meiotic outer plaque, and two-hybrid analyses have identified many interactions between these outer plaque proteins. However, precisely how these proteins are organized with respect to each other within the outer plaque is not known. The arrangement of these proteins within the outer plaque will be explored by FRET-based methodology.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 在减数分裂过程中，纺锤体极体（SPB）的外部斑块被重组。该结构从微管生长部位转变为膜形成和锚定部位。这些膜称为原孢子膜，对于孢子的形成至关重要。电子显微镜分析揭示了减数分裂外斑的高度组织结构，并且双杂交分析已经识别出这些外斑蛋白之间的许多相互作用。然而，这些蛋白质在外斑块内如何相互组织的确切方式尚不清楚。这些蛋白质在外斑块内的排列将通过基于 FRET 的方法进行探索。