Developmental Regulation of the Secretory Pathway in Yeast

酵母分泌途径的发育调控

• 批准号: 7929176
• 负责人: Aaron M Neiman
• 金额: $ 8.84万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7929176
• 关键词: Architecture; Binding; Biological; Carbon; Cell division; Cell membrane; Cells; Cellular Membrane; Chimeric Proteins; Collection; Complex; DNA Sequence Rearrangement; Data; Destinations; Development; Docking; Electron Microscopy; Enzymes; Eukaryotic Cell; Event; Excision; Face; Funding; Generations; Genes; Genetic; Growth; Individual; Intracellular Membranes; Investigation; Lecithin; Light; Lipids; Mediator of activation protein; Meiosis; Membrane; Membrane Fusion; Membrane Lipids; Modeling; Movement; Pathway interactions; Pattern; Phase; Phosphatidic Acid; Phospholipase D; Process; Proteins; Regulation; Reproduction spores; Role; SNAP receptor; Saccharomyces cerevisiae; Secretory Vesicles; Set protein; Site; Source; Specificity; Structure; System; Transport Vesicles; Vesicle; Work; Yeasts; base; cell type; in vivo; insight; membrane assembly; programs; response; spindle pole body; trafficking

The endomembrane system of all eukaryotic cells consists of a collection of membrane bound compartments. Movement of materials amongst these compartments is achieved by the regulated trafficking of cargo vesicles between compartments. In the process of differentiation, higher cells re-organize their internal membranes in an enormous variety of ways; expanding or specializing certain compartments, distributing them differently in the cell, or carrying vesicles to new destinations. Though these rearrangements are often critical to the function of the differentiated cell, little is known about how these specializations are imposed on the basic pattern of the secretory pathway. Spore formation in yeast involves a cell division that requires a similar organized rearrangement of the secretory apparatus. In this instance, retargetting of secretory vesicles gives rise to a new membrane compartment, the prospore membrane. This membrane arises by the redirection of secretory vesicles away from the plasma membrane to the cell interior. Prospore membrane formation therefore serves as a model for understanding the developmental^ programmed reorganization of cellular membranes. In addition to the retargetting of secretory vesicles, new genetic requirements are imposed on the fusion and trafficking of these vesicles during sporulation. During the initial coalescence of vesicles into a membrane sheet, fusion of the vesicles requires a specialized docking complex, specific fusion proteins, and a lipid modifying enzyme. We are using a combination of genetic and cell biological approaches to understand how these different activities interact to allow the de novo formation of a new membrane compartment. Once an initial membrane sheet is formed, fusion of vesicles to the membrane is controlled by a distinct set of proteins. The basis for this change is also under investigation. Together these studies will provide insight into how the cell modifies its basic architecture during differentiation.

所有真核细胞的内膜系统由膜结合的集合组成 隔间。材料在这些隔间之间的移动是通过受管制的贩运来实现的 隔室之间的货物囊泡。在分化过程中，高等细胞重新组织它们的 以多种方式形成内膜；扩大或专门化某些隔间， 它们在细胞中以不同的方式分布，或者将囊泡携带到新的目的地。虽然这些 重排通常对分化细胞的功能至关重要，但人们对这些重排如何发挥作用知之甚少。 分泌途径的基本模式具有特殊性。 酵母中孢子的形成涉及细胞分裂，需要类似的有组织的重排 分泌装置。在这种情况下，分泌囊泡的重新定位产生了新的膜 室，原孢子膜。该膜是由分泌囊泡重定向而产生的 从质膜到细胞内部。因此，原生孢子膜的形成可作为模型 用于理解细胞膜的发育程序重组。 除了分泌囊泡的重新定位之外，还对融合和融合提出了新的遗传要求。 这些囊泡在孢子形成过程中的运输。在囊泡最初合并成膜的过程中 表中，囊泡的融合需要专门的对接复合物、特定的融合蛋白和脂质 修饰酶。我们正在结合遗传和细胞生物学方法来了解如何 这些不同的活动相互作用，使新的膜区室从头形成。曾经一个 初始膜片形成后，囊泡与膜的融合由一组不同的控制 蛋白质。这一变化的依据也在调查中。这些研究将共同​​提供见解 研究细胞在分化过程中如何改变其基本结构。

项目成果

Genetic evidence of a role for membrane lipid composition in the regulation of soluble NEM-sensitive factor receptor function in Saccharomyces cerevisiae.

膜脂成分在酿酒酵母可溶性 NEM 敏感因子受体功能调节中的作用的遗传证据。

• DOI: 10.1534/genetics.166.1.89
• 发表时间: 2004
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Coluccio,Alison;Malzone,Maria;Neiman,AaronM
• 通讯作者: Neiman,AaronM

Binding interactions control SNARE specificity in vivo.

结合相互作用控制体内 SNARE 特异性。

• DOI: 10.1083/jcb.200809178
• 发表时间: 2008
• 期刊: The Journal of cell biology
• 作者: Yang,Hui-Ju;Nakanishi,Hideki;Liu,Song;McNew,JamesA;Neiman,AaronM
• 通讯作者: Neiman,AaronM

A guaninine nucleotide exchange factor is a component of the meiotic spindle pole body in Schizosaccharomyces pombe.

• DOI: 10.1091/mbc.e09-10-0842
• 发表时间: 2010-04-01
• 期刊: Molecular biology of the cell
• 影响因子: 3.3
• 作者: Yang HJ;Neiman AM
• 通讯作者: Neiman AM

A Gip1p-Glc7p phosphatase complex regulates septin organization and spore wall formation.

• DOI: 10.1083/jcb.200107008
• 发表时间: 2001-11-26
• 期刊: The Journal of cell biology
• 作者: Tachikawa H;Bloecher A;Tatchell K;Neiman AM
• 通讯作者: Neiman AM

Cdc15 is required for spore morphogenesis independently of Cdc14 in Saccharomyces cerevisiae.

在酿酒酵母中，Cdc15 是孢子形态发生所必需的，独立于 Cdc14。

• DOI: 10.1534/genetics.107.076133
• 发表时间: 2007
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Pablo-Hernando,MEvangelina;Arnaiz-Pita,Yolanda;Nakanishi,Hideki;Dawson,Dean;delRey,Francisco;Neiman,AaronM;VazquezdeAldana,CarlosR
• 通讯作者: VazquezdeAldana,CarlosR