Assembly and Function of the Yeast Spore Wall

酵母孢子壁的组装和功能

• 批准号: 7923497
• 负责人: Aaron M Neiman
• 金额: $ 19.27万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7923497
• 关键词: Antibodies; Appearance; Binding; Biological Assay; Biological Models; CDH1 gene; Cell Cycle; Cell Wall; Cells; Chitin; Chitin Synthase; Chitosan; Chromosome Segregation; Collection; Complex; Cytolysis; Cytoprotection; Defect; Deposition; Development; Diffuse; Enzymes; Extracellular Matrix; Extracellular Structure; Failure; Gene Mutation; Genes; Genetic; Glucans; Grant; Homologous Protein; Individual; Knock-out; Lectin; Link; Mediating; Meiosis; Membrane; Mitosis; Modeling; Molecular; Molecular Mechanisms of Action; Monitor; Mutate; Mutation; Organism; Pathway interactions; Pattern; Peptide Signal Sequences; Phenotype; Phosphorylation; Phosphorylation Site; Polysaccharides; Process; Proteins; Regulation; Regulator Genes; Reproduction spores; Research Personnel; Resistance; Role; Route; Saccharomyces cerevisiae; Site; Stress; Structure; Sum; Testing; Time; Transmembrane Domain; Ubiquitin-mediated Proteolysis Pathway; Work; Yeasts; base; beta-Glucans; cell type; crosslink; dityrosine; glucan synthase; inhibitor/antagonist; insight; mutant; novel; physical property; programs; protein transport; scaffold; sugar; yeast two hybrid system

All cells surround themselves with an extracellular matrix that provides both physical support and protection for the cell. In multicellular organisms the extracellular matrices from individual cells combine to form much larger structures that provide the scaffolding for the body of the organism. It is important, therefore, to understand how extracellular matrices are constructed and connected. The formation of the spore wall, a stratified, multicellular extracellular matrix, in the yeast Saccharomyces cerevisiae provides an excellent model system to study the assembly of extracellular matrices. An initial characterization of a collection of mutants defective in spore wall formation has provided an outline of the pathway of spore wall assembly. This initial work will be expanded by further studies on the role of the different spore wall assembly genes in promoting formation of the wall. The localization and activity of the beta-glucan and chitin syntheses, which catalyze the formation of the major spore wall polysaccharides, will be investigated both in wild type cells and in cells mutant for putative regulatory genes. Additionally, the molecular mechanism of action of the AMA1 gene, which links the exit from meiosis to the onset of spore wall formation will be explored. Finally, the outer layers of the spore wall both allow the spore to resist environmental stress and connect individual spores together through bridges. The targeting and anchoring to the spore wall of proteins involved in the assembly of these layers will be investigated. Also, a collection of mutants with modest defects in outer spore wall assembly will be analyzed to define genes involved in acquisition of resistance to specific stresses. In sum, these studies should deepen our understanding of the molecular mechanisms of spore wall construction, which will in turn provide insight into the strategies of extracellular matrix assembly in other organisms.

所有细胞周围都有细胞外基质，为细胞提供物理支持和保护。在多细胞生物中，单个细胞的细胞外基质结合形成更大的结构，为生物体提供支架。因此，了解细胞外基质的构建和连接方式非常重要。酿酒酵母中孢子壁（一种分层的多细胞胞外基质）的形成为研究胞外基质的组装提供了一个极好的模型系统。对孢子壁形成缺陷的突变体集合的初步表征提供了孢子壁组装途径的轮廓。这项初步工作将通过进一步研究不同孢子壁组装基因在促进孢子壁形成中的作用来扩展。催化主要孢子壁多糖形成的β-葡聚糖和几丁质合成的定位和活性将在野生型细胞和突变细胞中研究推定的调节基因。此外，还将探讨 AMA1 基因的分子作用机制，该基因将减数分裂的退出与孢子壁形成的开始联系起来。最后，孢子壁的外层既使孢子能够抵抗环境压力，又通过桥梁将各个孢子连接在一起。将研究参与这些层组装的蛋白质的靶向和锚定到孢子壁。此外，还将对外孢子壁组装中具有适度缺陷的突变体集合进行分析，以定义参与获得对特定胁迫的抗性的基因。总之，这些研究应该加深我们对孢子壁构建的分子机制的理解，进而为其他生物体细胞外基质组装的策略提供见解。