Assembly and Function of the Yeast Spore Wall
酵母孢子壁的组装和功能
基本信息
- 批准号:7923497
- 负责人:
- 金额:$ 19.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-09-30 至 2010-08-31
- 项目状态:已结题
- 来源:
- 关键词:AntibodiesAppearanceBindingBiological AssayBiological ModelsCDH1 geneCell CycleCell WallCellsChitinChitin SynthaseChitosanChromosome SegregationCollectionComplexCytolysisCytoprotectionDefectDepositionDevelopmentDiffuseEnzymesExtracellular MatrixExtracellular StructureFailureGene MutationGenesGeneticGlucansGrantHomologous ProteinIndividualKnock-outLectinLinkMediatingMeiosisMembraneMitosisModelingMolecularMolecular Mechanisms of ActionMonitorMutateMutationOrganismPathway interactionsPatternPeptide Signal SequencesPhenotypePhosphorylationPhosphorylation SitePolysaccharidesProcessProteinsRegulationRegulator GenesReproduction sporesResearch PersonnelResistanceRoleRouteSaccharomyces cerevisiaeSiteStressStructureSumTestingTimeTransmembrane DomainUbiquitin-mediated Proteolysis PathwayWorkYeastsbasebeta-Glucanscell typecrosslinkdityrosineglucan synthaseinhibitor/antagonistinsightmutantnovelphysical propertyprogramsprotein transportscaffoldsugaryeast two hybrid system
项目摘要
All cells surround themselves with an extracellular matrix that provides both physical support and protection for the cell. In multicellular organisms the extracellular matrices from individual cells combine to form much larger structures that provide the scaffolding for the body of the organism. It is important, therefore, to understand how extracellular matrices are constructed and connected. The formation of the spore wall, a stratified, multicellular extracellular matrix, in the yeast Saccharomyces cerevisiae provides an excellent model system to study the assembly of extracellular matrices. An initial characterization of a collection of mutants defective in spore wall formation has provided an outline of the pathway of spore wall assembly. This initial work will be expanded by further studies on the role of the different spore wall assembly genes in promoting formation of the wall. The localization and activity of the beta-glucan and chitin syntheses, which catalyze the formation of the major spore wall polysaccharides, will be investigated both in wild type cells and in cells mutant for putative regulatory genes. Additionally, the molecular mechanism of action of the AMA1 gene, which links the exit from meiosis to the onset of spore wall formation will be explored. Finally, the outer layers of the spore wall both allow the spore to resist environmental stress and connect individual spores together through bridges. The targeting and anchoring to the spore wall of proteins involved in the assembly of these layers will be investigated. Also, a collection of mutants with modest defects in outer spore wall assembly will be analyzed to define genes involved in acquisition of resistance to specific stresses. In sum, these studies should deepen our understanding of the molecular mechanisms of spore wall construction, which will in turn provide insight into the strategies of extracellular matrix assembly in other organisms.
所有细胞都被细胞外基质包围,为细胞提供物理支持和保护。在多细胞生物体中,来自单个细胞的细胞外基质联合收割机形成更大的结构,为生物体提供支架。因此,了解细胞外基质是如何构建和连接的是很重要的。酿酒酵母孢子壁是一种分层的多细胞胞外基质,它的形成为研究胞外基质的组装提供了一个很好的模型系统。一个收集的突变体缺陷的孢子壁形成的初步表征提供了一个轮廓的孢子壁组装的途径。通过进一步研究不同孢子壁组装基因在促进壁形成中的作用,这一初步工作将得到扩展。将在野生型细胞和假定调控基因的细胞突变体中研究β-葡聚糖和几丁质合成的定位和活性,所述β-葡聚糖和几丁质合成催化主要孢子壁多糖的形成。此外,AMA 1基因的分子作用机制,它连接从减数分裂的孢子壁形成的开始退出将被探索。最后,孢子壁的外层既允许孢子抵抗环境压力,又通过桥梁将单个孢子连接在一起。将研究这些层的组装中涉及的蛋白质的孢子壁的靶向和锚定。此外,将分析在孢子外壁组装中具有适度缺陷的突变体的集合,以确定参与获得对特定胁迫的抗性的基因。总之,这些研究将加深我们对孢子壁构建的分子机制的理解,这反过来将为其他生物体中细胞外基质组装的策略提供见解。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Aaron M Neiman其他文献
Aaron M Neiman的其他文献
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{{ truncateString('Aaron M Neiman', 18)}}的其他基金
INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY
减数分裂纺锤体蛋白质之间的相互作用
- 批准号:
8365796 - 财政年份:2011
- 资助金额:
$ 19.27万 - 项目类别:
Chromatin and the Control of Late Meiotic Gene Expression
染色质和减数分裂晚期基因表达的控制
- 批准号:
7917077 - 财政年份:2010
- 资助金额:
$ 19.27万 - 项目类别:
TRAINING IN LIVE CELL FLUORESCENCE MICROSCOPY AND FRET ANALYSIS
活细胞荧光显微镜和微丝分析培训
- 批准号:
7957817 - 财政年份:2009
- 资助金额:
$ 19.27万 - 项目类别:
Developmental Regulation of the Secretory Pathway in Yeast
酵母分泌途径的发育调控
- 批准号:
7929176 - 财政年份:2009
- 资助金额:
$ 19.27万 - 项目类别:
INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY
减数分裂纺锤体蛋白质之间的相互作用
- 批准号:
7723749 - 财政年份:2008
- 资助金额:
$ 19.27万 - 项目类别:
TRAINING IN LIVE CELL FLUORESCENCE MICROSCOPY AND FRET ANALYSIS
活细胞荧光显微镜和微丝分析培训
- 批准号:
7723719 - 财政年份:2008
- 资助金额:
$ 19.27万 - 项目类别:
INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY
减数分裂纺锤体蛋白质之间的相互作用
- 批准号:
7420716 - 财政年份:2006
- 资助金额:
$ 19.27万 - 项目类别:
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