FLUORESCENT ANALYSIS OF REGULATION OF CHOLESTEROL METABOLISM IN MAMMALIAN CELL

哺乳动物细胞胆固醇代谢调节的荧光分析

• 批准号: 7365585
• 负责人: VICKIE J LAMORTE
• 金额: $ 2.65万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7365585
• 关键词: FLUORESCENT; ANALYSIS; REGULATION; CHOLESTEROL; METABOLISM

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major focus of our research program is to understand how cholesterol metabolism is regulated in higher animals. Cholesterol is a major structural molecule of the plasma membranes of virtually all mammalian cells. Its unique chemical and geometric qualities makes it ideal for its role in membrane function since it provides structural rigidity and aids in the selective permeability of the lipid bilayer. In addition, cholesterol plays an essential role in more specialized biological processes. It is a major component of the very low density lipoprotein particle which is secreted by the liver for delivery of cholesterol and triacylglycerols to other sites in the body; and cholesterol is a required precursor in the synthesis of steroid hormones and bile acids which are essential for proper nutrition, growth, and development. Also, the excellent insulating capacity of the cholesterol molecule is exploited for the formation of the myelin sheath that wraps axons a nd is essential to rapid propagation of action potentials in the nervous system. Additionally, an exciting new report has demonstrated that cholesterol is covalently added to the "hedgehog" family of small proteins during a proteolytic maturation process that is required for these proteins to act as important signaling molecules during pattern formation of the developing embryo (Porter et al., 1996). This is an exciting and novel role for the cholesterol molecule.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们研究计划的主要重点是了解高等动物胆固醇代谢是如何调节的。胆固醇是几乎所有哺乳动物细胞质膜的主要结构分子。其独特的化学和几何性质使其成为膜功能的理想选择，因为它提供了结构刚性并有助于脂质双层的选择性渗透。此外，胆固醇在更专门的生物过程中起着至关重要的作用。它是极低密度脂蛋白颗粒的主要成分，极低密度脂蛋白颗粒由肝脏分泌，用于将胆固醇和三酰甘油输送到体内其他部位;胆固醇是合成类固醇激素和胆汁酸所需的前体，类固醇激素和胆汁酸对适当的营养、生长和发育至关重要。此外，胆固醇分子的优异绝缘能力被用于形成包裹轴突的髓鞘，并且对于神经系统中动作电位的快速传播至关重要。此外，一份令人兴奋的新报告已经证明，胆固醇在蛋白水解成熟过程中被共价添加到小蛋白的“刺猬”家族，这是这些蛋白质在发育胚胎的模式形成期间充当重要信号分子所需的（Porter等人，1996年）的报告。这对胆固醇分子来说是一个令人兴奋的新角色。