Zeiss LMS 510 META NLO Multi-Photon System

描述（由申请人提供）：我们是一个由13名多学科研究人员组成的小组，代表加州大学欧文分校的10个系，其中9个联邦资助的研究项目受到仪器申请的影响。我们正在为Zeiss LSM 51 0 Meta NLO多光子显微镜寻求资金，该显微镜将在Beckman激光研究所的NIH-NCRR生物医学技术资源中心（P41-RR 01192，激光微束和医疗计划，LAMMP）内安装和支持。LAMMP设施致力于定义和理解与使用光作为选择性干扰，监测和成像细胞和组织生理学的工具有关的广泛问题。迫切需要一个商业化的，用户友好的共聚焦/多光子显微镜，以容纳和管理在我们的NIH资源。所有拟议的试验/可行性研究均使用非商业化的自制多光子激发激光扫描系统和/或14年历史的LSM 410共焦系统。这些项目的范围已经扩大，现在有了校外的联邦支持，反过来，需要经常和经常大量使用激光扫描显微镜。为了充分适应这些创新研究的成熟，我们正在申请资金购买蔡司Meta仪器。该平台理想地满足了我们的需求，从组织中荧光和二次谐波产生（SHG）的低光水平内源性信号检测到基于FRET的细胞成像。虽然LAMMP设施包括多光子和共聚焦显微镜，但这些仪器是定制的，低通量设备，针对特殊需求和试点/可行性数据进行了优化。因此，这些NIH资助的研究人员在无法获得商业级仪器的情况下，继续取得进展受到阻碍。预计所要求的仪器将有一个显着的整体效益，以UCI研究界和超越现有的强大影响的LAMMP及其传播的新发现的同事和合作者。

DESCRIPTION (provided by applicant): We are a group of 13 multi-disciplinary investigators representing ten departments at the University of California, Irvine with 9 federally funded research projects impacted by this request for instrumentation. We are seeking funds for a Zeiss LSM 51 0 META NLO multi-photon microscope to be housed and supported within the well-established NIH-NCRR Biomedical Technology Resource Center (P41-RR01192, Laser Microbeam and Medical Program, LAMMP) at the Beckman Laser Institute. The LAMMP facility is dedicated to defining and understanding broad issues related to the use of light as a tool to selectively perturb, monitor, and image physiology in cells and tissues. There is an urgent need for a commercial, user-friendly confocal/multi-photon microscope to be housed and managed within our NIH resource. All of the proposed as pilot/feasibility studies using a non-commercial, home-built multi-photon excitation laser scanning system and/or a 14-year-old LSM 410 confocal system. These projects have expanded in scope, and now have extramural federal support that, in turn, requires frequent and often heavy use of laser scanning microscopy. In order to adequately accommodate the maturation of these innovative studies, we are requesting funds to purchase a Zeiss META instrument. This platform ideally accommodates our needs, which range from low-light-level endogenous signal detection of both fluorescence and second harmonic generation (SHG) in tissues to cellular FRET-based imaging. Although LAMMP facilities include multi-photon and confocal microscopy, these instruments are custom-built, low-throughput devices optimized for special needs and pilot/feasibility data. Consequently, continued progress for these NIH-funded investigators is hampered without access to a commercial-grade instrument. It is expected that the requested instrumentation will have a significant overall benefit to the UCI research community and beyond based on the existing strong impact of the LAMMP and its dissemination of new discoveries to colleagues and collaborators.