Zeiss LMS 510 META NLO Multi-Photon System

蔡司 LMS 510 META NLO 多光子系统

• 批准号: 7038113
• 负责人: VICKIE J LAMORTE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7038113
• 关键词: electromagnetic radiation; housing; lasers; lighting; microscopy; tissues

DESCRIPTION (provided by applicant): We are a group of 13 multi-disciplinary investigators representing ten departments at the University of California, Irvine with 9 federally funded research projects impacted by this request for instrumentation. We are seeking funds for a Zeiss LSM 51 0 META NLO multi-photon microscope to be housed and supported within the well-established NIH-NCRR Biomedical Technology Resource Center (P41-RR01192, Laser Microbeam and Medical Program, LAMMP) at the Beckman Laser Institute. The LAMMP facility is dedicated to defining and understanding broad issues related to the use of light as a tool to selectively perturb, monitor, and image physiology in cells and tissues. There is an urgent need for a commercial, user-friendly confocal/multi-photon microscope to be housed and managed within our NIH resource. All of the proposed as pilot/feasibility studies using a non-commercial, home-built multi-photon excitation laser scanning system and/or a 14-year-old LSM 410 confocal system. These projects have expanded in scope, and now have extramural federal support that, in turn, requires frequent and often heavy use of laser scanning microscopy. In order to adequately accommodate the maturation of these innovative studies, we are requesting funds to purchase a Zeiss META instrument. This platform ideally accommodates our needs, which range from low-light-level endogenous signal detection of both fluorescence and second harmonic generation (SHG) in tissues to cellular FRET-based imaging. Although LAMMP facilities include multi-photon and confocal microscopy, these instruments are custom-built, low-throughput devices optimized for special needs and pilot/feasibility data. Consequently, continued progress for these NIH-funded investigators is hampered without access to a commercial-grade instrument. It is expected that the requested instrumentation will have a significant overall benefit to the UCI research community and beyond based on the existing strong impact of the LAMMP and its dissemination of new discoveries to colleagues and collaborators.

描述（由申请人提供）：我们是一个由 13 名多学科研究人员组成的小组，代表加州大学欧文分校的 10 个系，有 9 个联邦资助的研究项目受到此仪器要求的影响。我们正在为蔡司 LSM 51 0 META NLO 多光子显微镜寻求资金，以便在贝克曼激光研究所完善的 NIH-NCRR 生物医学技术资源中心（P41-RR01192，激光微束和医疗计划，LAMMP）内安置和支持。 LAMMP 设施致力于定义和理解与使用光作为选择性扰动、监测和成像细胞和组织生理学的工具相关的广泛问题。迫切需要在我们的 NIH 资源中安置和管理商业化、用户友好的共焦/多光子显微镜。所有拟议的试点/可行性研究均使用非商业的自制多光子激发激光扫描系统和/或已有 14 年历史的 LSM 410 共焦系统。这些项目的范围已经扩大，现在得到了联邦政府的外部支持，而这反过来又需要频繁且经常大量使用激光扫描显微镜。为了充分适应这些创新研究的成熟，我们请求资金购买蔡司 META 仪器。该平台非常适合我们的需求，从组织中荧光和二次谐波产生 (SHG) 的低光内源信号检测到基于细胞 FRET 的成像。尽管 LAMMP 设施包括多光子和共焦显微镜，但这些仪器是定制的低通量设备，针对特殊需求和试点/可行性数据进行了优化。因此，如果无法使用商业级仪器，这些 NIH 资助的研究人员的持续进展就会受到阻碍。基于 LAMMP 现有的强大影响及其向同事和合作者传播新发现，预计所需的仪器将为 UCI 研究界及其他领域带来重大的整体利益。

项目成果

Fluorescent hydrogels for embryoid body formation and osteogenic differentiation of embryonic stem cells.

• DOI: 10.1021/acsami.5b02368
• 发表时间: 2015-05
• 期刊: ACS applied materials & interfaces
• 影响因子: 9.5
• 作者: N. Z. Zur Nieden;Cassandra C. Turgman;Xuye Lang;Jillian M. Larsen;Joseph Granelli;Yu-Jer Hwang;Julia G. Lyubovitsky