Zeiss LMS 510 META NLO Multi-Photon System

蔡司 LMS 510 META NLO 多光子系统

• 批准号: 7038113
• 负责人: VICKIE J LAMORTE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7038113
• 关键词: electromagnetic radiation; housing; lasers; lighting; microscopy; tissues

DESCRIPTION (provided by applicant): We are a group of 13 multi-disciplinary investigators representing ten departments at the University of California, Irvine with 9 federally funded research projects impacted by this request for instrumentation. We are seeking funds for a Zeiss LSM 51 0 META NLO multi-photon microscope to be housed and supported within the well-established NIH-NCRR Biomedical Technology Resource Center (P41-RR01192, Laser Microbeam and Medical Program, LAMMP) at the Beckman Laser Institute. The LAMMP facility is dedicated to defining and understanding broad issues related to the use of light as a tool to selectively perturb, monitor, and image physiology in cells and tissues. There is an urgent need for a commercial, user-friendly confocal/multi-photon microscope to be housed and managed within our NIH resource. All of the proposed as pilot/feasibility studies using a non-commercial, home-built multi-photon excitation laser scanning system and/or a 14-year-old LSM 410 confocal system. These projects have expanded in scope, and now have extramural federal support that, in turn, requires frequent and often heavy use of laser scanning microscopy. In order to adequately accommodate the maturation of these innovative studies, we are requesting funds to purchase a Zeiss META instrument. This platform ideally accommodates our needs, which range from low-light-level endogenous signal detection of both fluorescence and second harmonic generation (SHG) in tissues to cellular FRET-based imaging. Although LAMMP facilities include multi-photon and confocal microscopy, these instruments are custom-built, low-throughput devices optimized for special needs and pilot/feasibility data. Consequently, continued progress for these NIH-funded investigators is hampered without access to a commercial-grade instrument. It is expected that the requested instrumentation will have a significant overall benefit to the UCI research community and beyond based on the existing strong impact of the LAMMP and its dissemination of new discoveries to colleagues and collaborators.

描述（由申请人提供）：我们是由13个多学科研究人员组成的小组，代表加利福尼亚大学尔湾分校的十个部门，由9个联邦政府资助的研究项目受到此仪器要求的影响。我们正在寻找Zeiss LSM 51 0 MetA NLO多光子显微镜的资金，该显微镜将在贝克曼·拉瑟学院（Beckman Laser Institute）置于良好成熟的NIH-NCRR生物医学技术资源中心（P41-RRRR01192，Laser Microbeam和Medical Program，LAMMP）中。 LAMMP设施致力于定义和理解与使用光作为在细胞和组织中选择性扰动，监测和图像生理的工具有关的广泛问题。迫切需要在我们的NIH资源中安装和管理商业，用户友好的共聚焦/多光子显微镜。所有提议的作为试验/可行性研究，都使用非商业，自制的多光子激发激光扫描系统和/或14岁的LSM 410共共聚焦系统。这些项目的范围扩大了，现在拥有壁外联邦支持，反过来又需要频繁且经常大量使用激光扫描显微镜。为了充分适应这些创新研究的成熟，我们要求资金购买Zeiss Meta仪器。理想情况下，该平台可满足我们的需求，其需求范围从荧光的低光内源信号检测到组织中的荧光和第二次谐波产生（SHG）到基于细胞FRET的成像。尽管LAMMP设施包括多光子和共聚焦显微镜，但这些仪器是针对特殊需求和飞行员/可行性数据进行了定制的低通量设备。因此，这些由NIH资助的调查人员的持续进展受到阻碍，而无需获得商业级工具。预计所要求的仪器将根据LAMMP的现有强烈影响及其向同事和合作者传播新发现对UCI研究界及其他地区的总体好处。

项目成果

Fluorescent hydrogels for embryoid body formation and osteogenic differentiation of embryonic stem cells.

• DOI: 10.1021/acsami.5b02368
• 发表时间: 2015-05
• 期刊: ACS applied materials & interfaces
• 影响因子: 9.5
• 作者: N. Z. Zur Nieden;Cassandra C. Turgman;Xuye Lang;Jillian M. Larsen;Joseph Granelli;Yu-Jer Hwang;Julia G. Lyubovitsky