ZEISS LMS 510 META NLO MULTI-PHOTON SYSTEM

ZEISS LMS 510 META NLO 多光子系统

• 批准号: 7335297
• 负责人: VICKIE J LAMORTE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335297
• 关键词: electromagnetic radiation; housing; lasers; lighting; microscopy; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are a group of 13 multi-disciplinary investigators representing ten departments at the University of California, Irvine with 9 federally funded research projects impacted by this request for instrumentation. We are seeking funds for a Zeiss LSM 51 0 META NLO multi-photon microscope to be housed and supported within the well-established NIH-NCRR Biomedical Technology Resource Center (P41-RR01192, Laser Microbeam and Medical Program, LAMMP) at the Beckman Laser Institute. The LAMMP facility is dedicated to defining and understanding broad issues related to the use of light as a tool to selectively perturb, monitor, and image physiology in cells and tissues. There is an urgent need for a commercial, user-friendly confocal/multi-photon microscope to be housed and managed within our NIH resource. All of the proposed as pilot/feasibility studies using a non-commercial, home-built multi-photon excitation laser scanning system and/or a 14-year-old LSM 410 confocal system. These projects have expanded in scope, and now have extramural federal support that, in turn, requires frequent and often heavy use of laser scanning microscopy. In order to adequately accommodate the maturation of these innovative studies, we are requesting funds to purchase a Zeiss META instrument. This platform ideally accommodates our needs, which range from low-light-level endogenous signal detection of both fluorescence and second harmonic generation (SHG) in tissues to cellular FRET-based imaging. Although LAMMP facilities include multi-photon and confocal microscopy, these instruments are custom-built, low-throughput devices optimized for special needs and pilot/feasibility data. Consequently, continued progress for these NIH-funded investigators is hampered without access to a commercial-grade instrument. It is expected that the requested instrumentation will have a significant overall benefit to the UCI research community and beyond based on the existing strong impact of the LAMMP and its dissemination of new discoveries to colleagues and collaborators.

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：我们是一个由13名多学科研究人员组成的小组，代表加州大学欧文分校的10个部门，有9个联邦资助的研究项目受到本次仪器申请的影响。我们正在为一个蔡司LSM 51 0 META NLO多光子显微镜寻求资金，该显微镜将被安置在贝克曼激光研究所完善的NIH-NCRR生物医学技术资源中心（P41-RR01192，激光微束和医疗项目，LAMMP）内并得到支持。LAMMP设施致力于定义和理解与使用光作为选择性干扰、监测和成像细胞和组织生理学的工具相关的广泛问题。迫切需要一个商业的，用户友好的共聚焦/多光子显微镜来安置和管理我们的NIH资源。所有建议的试点/可行性研究使用非商业的，自制的多光子激发激光扫描系统和/或14年的LSM 410共聚焦系统。这些项目已经扩大了范围，现在得到了联邦政府的外部支持，这反过来又需要频繁和经常大量使用激光扫描显微镜。为了充分适应这些创新研究的成熟，我们正在申请资金购买蔡司META仪器。该平台理想地满足了我们的需求，从组织中荧光和二次谐波产生（SHG）的微光内源性信号检测到基于细胞fret的成像。虽然LAMMP设备包括多光子和共聚焦显微镜，但这些仪器是定制的，低通量设备，针对特殊需求和试点/可行性数据进行了优化。因此，这些美国国立卫生研究院资助的研究人员在没有获得商业级仪器的情况下，继续取得进展受到阻碍。基于LAMMP现有的强大影响及其向同事和合作者传播新发现，预计所要求的仪器将对UCI研究界和其他领域产生重大的总体效益。