ZEISS LMS 510 META NLO MULTI-PHOTON SYSTEM

ZEISS LMS 510 META NLO 多光子系统

• 批准号: 7335297
• 负责人: VICKIE J LAMORTE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335297
• 关键词: electromagnetic radiation; housing; lasers; lighting; microscopy; tissues

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are a group of 13 multi-disciplinary investigators representing ten departments at the University of California, Irvine with 9 federally funded research projects impacted by this request for instrumentation. We are seeking funds for a Zeiss LSM 51 0 META NLO multi-photon microscope to be housed and supported within the well-established NIH-NCRR Biomedical Technology Resource Center (P41-RR01192, Laser Microbeam and Medical Program, LAMMP) at the Beckman Laser Institute. The LAMMP facility is dedicated to defining and understanding broad issues related to the use of light as a tool to selectively perturb, monitor, and image physiology in cells and tissues. There is an urgent need for a commercial, user-friendly confocal/multi-photon microscope to be housed and managed within our NIH resource. All of the proposed as pilot/feasibility studies using a non-commercial, home-built multi-photon excitation laser scanning system and/or a 14-year-old LSM 410 confocal system. These projects have expanded in scope, and now have extramural federal support that, in turn, requires frequent and often heavy use of laser scanning microscopy. In order to adequately accommodate the maturation of these innovative studies, we are requesting funds to purchase a Zeiss META instrument. This platform ideally accommodates our needs, which range from low-light-level endogenous signal detection of both fluorescence and second harmonic generation (SHG) in tissues to cellular FRET-based imaging. Although LAMMP facilities include multi-photon and confocal microscopy, these instruments are custom-built, low-throughput devices optimized for special needs and pilot/feasibility data. Consequently, continued progress for these NIH-funded investigators is hampered without access to a commercial-grade instrument. It is expected that the requested instrumentation will have a significant overall benefit to the UCI research community and beyond based on the existing strong impact of the LAMMP and its dissemination of new discoveries to colleagues and collaborators.

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(由申请人提供)：我们是一个由13名多学科调查人员组成的小组，代表加州大学欧文分校的10个系，有9个联邦资助的研究项目受到此仪器请求的影响。我们正在为蔡司LSM 51 0 Meta NLO多光子显微镜寻求资金，该显微镜将安装在贝克曼激光研究所久负盛名的NIH-NCRR生物医学技术资源中心(P41-RR01192，激光微束和医学计划，LAMMP)内并得到支持。LAMP设施致力于定义和了解与使用光作为选择性干扰、监测和成像细胞和组织中的生理学的工具有关的广泛问题。我们迫切需要一种商业的、用户友好的共焦/多光子显微镜，并在我们的NIH资源中进行容纳和管理。所有建议作为试点/可行性研究，使用非商业的、自制的多光子激发激光扫描系统和/或已有14年历史的LSM 410共聚焦系统。这些项目的范围已经扩大，现在得到了外部联邦的支持，这反过来又需要频繁且经常大量使用激光扫描显微镜。为了充分适应这些创新研究的成熟，我们正在申请购买蔡司Meta仪器的资金。该平台完美地满足了我们的需求，从对组织中荧光和二次谐波(SHG)的微光内源信号检测到基于细胞FRET的成像。尽管LAMP设施包括多光子和共聚焦显微镜，但这些仪器是定制的低通量设备，针对特殊需求和试验/可行性数据进行了优化。因此，这些由美国国立卫生研究院资助的研究人员在无法获得商业级仪器的情况下，继续取得进展受到阻碍。预计所要求的仪器将对UCI研究界和其他方面产生重大的整体效益，因为LAMP现有的强大影响及其向同事和合作者传播的新发现。