Application of an ultra fast single photon camera for spatial imaging of fast cellular events

超快单光子相机在快速细胞事件空间成像中的应用

• 批准号: BB/E000169/1
• 负责人: Colin Brownlee
• 金额: $ 16.96万
• 依托单位: Marine Biological Association of the United Kingdom
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE000169%2F1
• 关键词: Application; ultra; fast; single; photon

Recent advances in microscopy have enabled the activities of living cells to be studied with unprecedented detail. These advances include the development of fluorescent markers that can be used to label particular structures, chemical messengers and ion concentrations in cells, together with the development of techniques such as confocal microscopy that remove unwanted information such as out-of-focus blur from the microscope image. Many cellular processes result from the coordinated activity of a diverse array of molecules, such as ion channels in the cell membranes, the components of the cell's skeleton and molecules involved in secretion and membrane turnover. Many of these interactions occur very fast, in multiple locations in the cell and on time scales of milliseconds or less. Current microscopy techniques are generally not fast or sensitive enough to record them. Recent developments in camera and detector technology are producing a generation of ultra-fast and ultra-sensitive cameras that should provide much-needed solutions for imaging of very fast cellular events. This project will adapt such a camera for imaging of fast sub-cellular events in a model cellular system. The potential application of the technology to a wider range of microscopy applications will be assessed.

显微镜的最新进展使活细胞的活动能够以前所未有的细节进行研究。这些进展包括可用于标记细胞中特定结构、化学信使和离子浓度的荧光标记物的开发，以及诸如共聚焦显微镜之类的技术的开发，这些技术可以从显微镜图像中去除不需要的信息，例如离焦模糊。许多细胞过程是由多种分子的协调活动引起的，例如细胞膜中的离子通道、细胞骨架的组成部分以及参与分泌和膜周转的分子。这些相互作用中的许多发生得非常快，在细胞的多个位置和毫秒或更短的时间尺度上。目前的显微镜技术通常不够快或灵敏，无法记录它们。相机和检测器技术的最新发展正在产生一代超快和超灵敏的相机，这些相机应该为非常快速的细胞事件成像提供急需的解决方案。该项目将采用这样的相机在一个模型细胞系统中的快速亚细胞事件的成像。将评估该技术在更广泛的显微镜应用中的潜在应用。

项目成果

Imaging visible light with Medipix2.

使用 Medipix2 对可见光进行成像。

• DOI: 10.1063/1.3501385
• 发表时间: 2010
• 期刊: The Review of scientific instruments
• 作者: Mac Raighne A

Development of a high-speed single-photon pixellated detector for visible wavelengths

开发可见光波长的高速单光子像素化探测器

• DOI: 10.1016/j.nima.2009.03.152
• 发表时间: 2009
• 期刊: Accelerators, Spectrometers, Detectors and Associated Equipment
• 作者: Raighne A