JC VIRUS EXPRESSION IN THE OROPHARYNX AND HIV

JC 病毒在口咽部和 HIV 中的表达

• 批准号: 7379035
• 负责人: Joseph R Berger
• 金额: $ 4.43万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7379035
• 关键词: JC; VIRUS; EXPRESSION; OROPHARYNX; HIV

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study is an exploratory analysis of the difference in the prevalence of the JC Virus in oropharyngeal secretions and blood of healthy individuals, persons with Acquired Immune Deficiency Syndroms (AIDS), and pes with progressive multifocal leukoencephalopathy (PML). Saliva and throat washings as well as whole blood samples will be taken from each group. Saliva samples and throat washings will be analyzed for JCV using polymerase chain reaction (PCR). Whole blood samples will be collected and analyzed for JCV using PCR and for the presence of JCV in peripheral blood leukocytes (PBLs) using CD4 lymphocyte counts. These measures will be used to determine whether JCV is harbored in the oropharynx, whether a correlation exists between JCV levels in the oropharynx and those in PBLs and to determine whether the level and strain of JCV shed from the oropharynx correlates with the degree of immunosuppression. It is predicted that JCV will be present in oropharyngeal fluids, the frequency of detection of JCV will be increased in patients with AIDS-associated PML compared with healthy controls and HIV-seropositive patients without PML and that higher levels of the JC Virus will correlate with increased immunosuppression.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本研究探索性分析了健康个体、获得性免疫缺陷综合征（AIDS）患者和进行性多灶性白质脑病（PML）患者的口咽分泌物和血液中JC病毒的患病率差异。将从每组中采集唾液和咽喉冲洗液以及全血样本。将使用聚合酶链反应（PCR）分析唾液样本和咽洗液中的JCV。将采集全血样本，并使用PCR分析JCV，使用CD 4淋巴细胞计数分析外周血白细胞（PBL）中是否存在JCV。这些测量将用于确定JCV是否存在于口咽中，口咽中的JCV水平与PBL中的JCV水平之间是否存在相关性，并确定从口咽脱落的JCV水平和菌株是否与免疫抑制程度相关。据预测，JCV将存在于口咽液中，与健康对照组和无PML的HIV血清阳性患者相比，艾滋病相关PML患者中JCV的检测频率将增加，并且更高水平的JC病毒将与增加的免疫抑制相关。