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Purchase of a 600 MHz ACAS magnet and cryogenic probe for high throughput metabolomics and ligand discovery

购买 600 MHz ACAS 磁体和低温探针，用于高通量代谢组学和配体发现

基本信息

批准号：
BB/E013198/1
负责人：
Michael Overduin
金额：
$ 31.16万
依托单位：
University of Birmingham
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2007
资助国家：
英国
起止时间：
2007 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FE013198%2F1
关键词：
Purchase 600 MHz ACAS magnet

项目摘要

This proposal requests funding to purchase a sample probe and superconducting magnet to be used for a magnetic resonance spectrometry at the University of Birmingham for scientific research by a broad range of users from across the United Kingdom. This probe and magnet will be used to characterize the structures and interactions of animal, plant and microbial proteins and metabolites. This information will be used to understand the functions of these molecules in solutions similar to those in the interior of a living organism or cell. In order to obtain such information, very weak electromagnetic signals originating from the hydrogen, nitrogen and carbon atoms of the protein must be detected. The requested probe offers the highest possible level of sensitivity for these experiments, allowing researchers to observe molecules that are rare, difficult or expensive to produce, or insufficiently soluble or stable at the high concentrations required for NMR analysis. The cryogenic probe allows one to either lower the concentration of the sample or reduce the time required to obtain NMR spectra of proteins. New experiments that are otherwise precluded by sensitivity requirements can be used, allowing larger proteins and rare small molecules to be detected and data to be collected more quickly. The requested magnet offers the latest in superconducting technology at a magnetic field strength of 14 Tesla. This magnet offers excellent separation of the hydrogen, nitrogen and carbon signals, and represents the state-of-the-art standard for metabolomics and ligand discovery research. In addition this magnet is actively cooled and actively shielded, meaning that it recycles helium and does not require weekly maintenance, thus reducing manpower costs in an environmentally friendly manner. The research that would be enabled by this probe focuses on proteins and biochemical systems which are involved in regulation of cell growth, and the control of the metabolic and signalling pathways within several organisms. The organisms to be studied include food-borne pathogenic bacteria, models for genomics such as Dictyostelium, aphids and the Arabidopsis plant, zebrafish, and mammalian tissues including the human brain. The availability of this probe and magnet would be a major draw for new users, allowing more experiments to be performed, better quality data to be collected, and new projects initiated and supported within a new national facility used for internationally competitive research in the post-genomic sciences.

该提案要求提供资金，用于购买一个样品探头和超导磁体，供伯明翰大学的磁共振光谱仪使用，供联合王国各地的广大用户进行科学研究。该探针和磁体将用于表征动物、植物和微生物蛋白质和代谢物的结构和相互作用。这些信息将用于了解这些分子在溶液中的功能，这些溶液类似于活生物体或细胞内部的溶液。为了获得这样的信息，必须检测源自蛋白质的氢、氮和碳原子的非常弱的电磁信号。所要求的探针为这些实验提供了尽可能高的灵敏度，使研究人员能够观察到稀有，难以或昂贵的分子，或者在NMR分析所需的高浓度下不充分溶解或稳定的分子。低温探针允许降低样品的浓度或减少获得蛋白质NMR谱所需的时间。可以使用因灵敏度要求而无法使用的新实验，从而可以检测到更大的蛋白质和罕见的小分子，并更快地收集数据。所要求的磁体提供最新的超导技术，磁场强度为14特斯拉。该磁体提供了氢、氮和碳信号的出色分离，代表了代谢组学和配体发现研究的最新标准。此外，该磁铁是主动冷却和主动屏蔽的，这意味着它可以吸收氦气，不需要每周维护，从而以环保的方式降低人力成本。该探针将使研究重点放在参与细胞生长调节的蛋白质和生化系统，以及几种生物体内代谢和信号通路的控制。待研究的生物体包括食源性致病菌、基因组学模型（如网骨藻、蚜虫和拟南芥）、斑马鱼和哺乳动物组织（包括人脑）。这种探针和磁铁的可用性将是新用户的主要吸引力，允许进行更多的实验，收集更高质量的数据，并在用于后基因组科学国际竞争研究的新国家设施内启动和支持新项目。