THE LUNG IN HIV DISEASE AND TUBERCULOSIS

HIV 疾病和结核病中的肺部

• 批准号: 7378008
• 负责人: Zahra Toossi Toossi
• 金额: $ 3.99万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7378008
• 关键词: LUNG; HIV; DISEASE; TUBERCULOSIS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In persons infected with human immunodeficiency virus-1 (HIV), the lung is a major target of opportunistic infection and non-infectious complications. Pulmonary tuberculosis (TB) accounts for 85% of cases of TB, and is common during HIV infection. TB may enhance HIV-related pulmonary complications, and expansion of the virus. The main objectives of this study are to examine the functions of alveolar macrophages from a spectrum of HIV infected patients and to quantitate the level of HIV in these cells. These studies attempt to examine the following: 1) The mechanisms by which TB activates bronchoalveolar cells to enhanced HIV activity, 2) The status of production and activity of cytokines and chemokines and their receptors in the lung, the MTB-infected macrophages and during HIV infection, 3) The mechanisms whereby M. tuberculosis (MTB) genes are modulated in infected alveolar macrophages from healthy subjects and in HIV-infected subjects. Bronchoscopies are performed on the GCRC to obtain cells used in this study. Broncholaveolar cells (BAC) and peripheral blood mononuclear cells (PBMC) will be obtained from HIV-infected subjects and uninfected healthy volunteers. Cells will be separated further in the laboratory and prepared for various analyses and experimentation in an

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在感染人类免疫缺陷病毒-1（HIV）的人群中，肺部是机会性感染和非感染性并发症的主要目标。肺结核（TB）占TB病例的85%，在HIV感染期间很常见。结核病可能会增加艾滋病毒相关的肺部并发症和病毒的扩散。本研究的主要目的是检查肺泡巨噬细胞的功能，从一个频谱的艾滋病毒感染的患者，并定量HIV在这些细胞中的水平。这些研究试图研究以下内容：1）结核病激活支气管肺泡细胞以增强艾滋病毒活性的机制，2）肺、结核病感染的巨噬细胞和艾滋病毒感染期间细胞因子和趋化因子及其受体的产生和活性状况，3）结核杆菌感染的机制。结核病（MTB）基因在来自健康受试者和HIV感染受试者的感染肺泡巨噬细胞中受到调节。对GCRC进行支气管镜检查以获得本研究中使用的细胞。 将从HIV感染受试者和未感染的健康志愿者中获得支气管肺泡细胞（BAC）和外周血单核细胞（PBMC）。细胞将在实验室中进一步分离，并准备用于各种分析和实验。