Virology, Proteomics and Microbial Pathogenesis

病毒学、蛋白质组学和微生物发病机制

• 批准号: 7930072
• 负责人: Zahra Toossi Toossi
• 金额: $ 29.8万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7930072
• 关键词: AIDS/HIV problem; Arts; Bioinformatics; Biological Assay; Cell Separation; Cells; Collection; Faculty; Funding; Genetic Recombination; Growth; HIV; HIV-1; Hepatitis C virus; Investigation; Laboratories; Life; Mass Spectrum Analysis; Measurement; Methodology; Methods; Monitor; Mononuclear; Mycobacterium tuberculosis; Pathogen detection; Pathogenesis; Pathway Analysis; Post-Translational Protein Processing; Production; Property; Proteins; Proteomics; Public Health; Recombinants; Research; Research Infrastructure; Research Personnel; Resources; Role; Safety; Sampling; Services; Systems Biology; Technology; Training; Uganda; United States National Institutes of Health; Virulent; Virus; Workplace; Yeasts; microbial; novel; pandemic disease; pathogen; recombinant virus; repository; virology

Core E, Virology, Proteomics and Microbial Pathogenesis (VPMP), is the main resource provided by the CFAR to support virological investigations of HIV and studies of its co-pathogens, Mycobacterium tuberculosis (MTB) and Hepatitis C Virus (HCV). The Core has been newly configured to provide a wide range of services in support of modern virology research that now includes mass spectrometry, live cell sorting, and proteomics, novel services for the rapid production of recombinant viruses, as well as state-of- the-art biosafety facilities, that include high throughput live cell sort facilities for infectious samples. The specific aims of the Virology, Proteomics and Microbial Pathogenesis core are: ¿ Virology: Provide recombinant HIV viruses using novel recombination methods in yeast recently developed by the Arts laboratory to rapidly generate viruses with desired properties. Provide access to a virus repository carrying a collection of over 500 well-characterized primary HIV isolates. Provide training to enhance the use of recombinant HlV-1 strains. ¿ Proteomics: Operate mass spectrometry, proteomics facilities and provide a systems biology and bioinformatics infrastructure to support a wide range of projects in HIV/AIDS research currently funded by the NIH. Specifically, proteomic services focus on protein abundance measurements, identification of post-translational modifications, structural proteomics analysis, and pathway analysis. Provide training to enhance the use and understanding of role of advanced Proteomics and Bioinformatics technologies in HIV research. ¿ Microbial Pathogenesis: Provide fully managed, well-equipped, and safe working environments (Biosafety level 2+ and 3) for the study of HIV, virulent MTB, and HCV, including live cell sorting (LCS) facilities for separation of HIV-infected, HCV-infected, and MTB-infected mononuclear cells. Safety monitoring, management, and training will be provide to the entire CFAR including the Uganda Laboratory Core C. Develop and provide quantitative PCR assays to monitor pathogen growth. Train and monitor researchers in Biosafety laboratories, and in the use of PCR methodologies for pathogen detection.

核心E，即病毒学、蛋白质组学和微生物致病学(VPMP)，是CFAR提供的主要资源，用于支持艾滋病毒的病毒学调查及其共病原体、结核分枝杆菌(MTB)和丙型肝炎病毒(HCV)的研究。核心已被新配置为提供广泛的服务，以支持现代病毒学研究，现在包括质谱学、活细胞分选和蛋白质组学，用于快速生产重组病毒的新服务，以及状态 最先进的生物安全设施，包括用于传染病样本的高通量活细胞分类设施。病毒学、蛋白质组学和微生物致病学核心的具体目标是： ？病毒学：使用Art实验室最近开发的在酵母中重组的新方法提供重组HIV病毒，以快速生成具有所需特性的病毒。提供对携带500多个特征良好的初级艾滋病毒分离株集合的病毒库的访问。提供培训以加强重组HLV-1毒株的使用。 蛋白质组学：运营质谱学、蛋白质组学设施，并提供系统生物学和生物信息学基础设施，以支持目前由国家卫生研究院资助的一系列艾滋病毒/艾滋病研究项目。具体地说，蛋白质组学服务侧重于蛋白质丰度测量、翻译后修饰的鉴定、结构蛋白质组学分析和途径。 分析。提供培训，以提高对先进蛋白质组学和生物信息学技术在艾滋病毒研究中的作用的使用和了解。 微生物致病：为HIV、毒力结核杆菌和丙型肝炎病毒的研究提供全面管理、设备齐全和安全的工作环境(生物安全级别2+和3级)，包括用于分离感染艾滋病毒、感染丙型肝炎病毒和感染结核杆菌的单核细胞的活细胞分选(LCS)设施。将向整个CFAR提供安全监测、管理和培训，包括 乌干达实验室核心C开发并提供定量的聚合酶链式反应分析，以监测病原体的生长。培训和监督生物安全实验室的研究人员，并使用聚合酶链式反应方法进行病原体检测。