Biosafety

生物安全核心的使命是双重的。首先也是最重要的是，核心提供完全管理、配备和 安全的工作环境，用于研究艾滋病毒、结核分枝杆菌病(MTB)和其他传染性病原体。 其次，核心积极开发最先进的分子方法来评估宿主/病原体 翻译应用程序的互动，例如开发实时定量PCR方法 研究样本中艾滋病毒复制和多样性的研究。此外，核心人员已经承担了 负责对所有用户进行实验室良好操作规范的培训，定期进行再培训以确保符合实验室要求 并提供服务，促进CWRU/UHC研究界所有调查人员的研究。 专门的BL-3和BL-2+实验室位于生物医学中心10楼的对面 传染病科的研究大楼。由五名成员组成的生物安全委员会开会监督 安全程序，一个六人科学小组开会，以确定核心在一个 四分音基。 这一核心的活动对CFAR的成功至关重要，因为在生物危险调查中既安全 病原体和评估宿主/艾滋病毒相互作用的新方法的发展是艾滋病的关键因素 CWRU/UHC的研究工作。核心的独特方面(即，研究艾滋病毒与艾滋病毒之间相互作用的能力 和致病的结核分枝杆菌)，强调了这一核心作为 CWRU/UHC CFAR。

The mission of the Biosafety Core is two-fold. First and foremost, the core provides a fully managed, equipped, and safe working environment for the study of HIV, Mycobactetium tubercu/osis (MTB) and other infectious pathogens. Secondly, the core actively develops state-of-the-art molecular methodologies for assessment of host/pathogen interactions for translational applications, such as the development of real-time quantitative PCR methodologies for the study of HIV replication and diversity in research samples. In addition, the core personnel have assumed the responsibility for training all users in good laboratory practices, periodic retraining to assure compliance with laboratory regulations, and providing services to promote research by all investigators in the CWRU/UHC research community. Dedicated BL-3 and BL-2+ laboratories are located across from each other on the 10th floor of the Biomedical Research Building in the Division of Infectious Diseases. A five-member Biosafety Committee meets to supervise safety procedures, and a six-member Scientific Team meets to determine developmental direction by the core on a quartedy basis. The activities of this core are essential to the success of CFAR, as both safety in investigation of biohazardous pathogens and development of new methodologies to assess host/HIV interactions constitute key elements in the AIDS research efforts at CWRU/UHC. The unique aspects of the core (i.e., the ability to study the interaction between HIV and virulent Mycobacterium tuberculosis), underscores the importance of this core as a major research strength of the CWRU/UHC CFAR.