CORE--Biosafety Core

CORE--生物安全核心

• 批准号: 6802582
• 负责人: Zahra Toossi Toossi
• 金额: $ 13.77万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6802582
• 关键词: AIDS; Mycobacterium tuberculosis; biohazard control; biomedical facility; human immunodeficiency virus; human tissue; microorganism culture; occupational health /safety; polymerase chain reaction; virus infection mechanism

The mission of the Biosafety Core is two-fold. First and foremost, the core provides a fully managed, equipped, and safe working environment for the study of HIV, Mycobacterium tuberculosis (MTB) and other infectious pathogens. Secondly, the core actively develops state-of-the-art molecular methodologies for assessment of host/pathogen interactions for translational applications, such as the development of real-time quantitative PCR methodologies for the study of HIV replication and diversity in research samples. In addition, the core personnel have assumed the responsibility for training all users in good laboratory practices, periodic retraining to assure compliance with laboratory regulations, and providing services to promote research by all investigators in the CWRU/UHC research community. Dedicated BL-3 and BL-2+ laboratories are located across from each other on the 10th floor of the Biomedical Research Building in the Division of Infectious Diseases. A five-member Biosafety Committee meets to supervise safety procedures, and a six-member Scientific Team meets to determine developmental direction by the core on a quarterly basis. The activities of this core are essential to the success of CFAR, as both safety in investigation of biohazardous pathogens and development of new methodologies to assess host/HIV interactions constitute key elements in the AIDS research efforts at CWRU/UHC. The unique aspects of the core (i.e., the ability to study the interaction between HIV and virulent Mycobacterium tuberculosis), underscores the importance of this core as a major research strength of the CWRU/UHC CFAR.

生物安全核心的使命是双重的。首先，核心为艾滋病毒、结核分枝杆菌(MTB)和其他传染性病原体的研究提供了一个全面管理、配备齐全和安全的工作环境。其次，该中心积极开发最先进的分子方法，用于评估宿主/病原体相互作用的翻译应用，例如开发实时定量聚合酶链式反应方法，用于研究研究样本中的艾滋病毒复制和多样性。此外，核心人员承担了对所有用户进行良好实验室做法培训的责任，定期进行再培训以确保遵守实验室规定，并提供服务以促进CWRU/UHC研究界所有研究人员的研究。专门的BL-3和BL-2+实验室位于生物医学中心10楼的对面 传染病科的研究大楼。一个由五名成员组成的生物安全委员会开会监督安全程序，一个由六名成员组成的科学小组每季度开会确定核心的发展方向。这一核心的活动对CFAR的成功至关重要，因为生物危险病原体调查的安全性和评估宿主/艾滋病毒相互作用的新方法的开发都是CWRU/UHC艾滋病研究工作的关键要素。核心的独特方面(即研究艾滋病毒和致病结核分枝杆菌之间相互作用的能力)突显了这一核心作为CWRU/UHC CFAR的主要研究力量的重要性。