Bulk culture of commercially viable embryonic stem cells.

商业上可行的胚胎干细胞的大量培养。

• 批准号: BB/E527239/1
• 负责人: Christopher Ward
• 金额: $ 11.5万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE527239%2F1
• 关键词: Bulk; culture; commercially; viable; embryonic

Dr Ward has developed and patented an enabling technology that permits the growth of embryonic stem cells in high density 3D cultures without the need for potentially harmful mouse feeder lines and expensive growth factors. The technology helps maintain ES cells in an undifferentiated state for prolonged periods (at least 30 days) and can significantly reduce the technical burden associated with current methods. By offering a route to simple, cost effective large volume culture of both mouse and human ES cells we will help to transform the utility of stem cells and widen their use as research tools and ultimately as therapeutic treatments of degenerative diseases. The objectives of this study are:- 1) Optimize and refine methods fro 3D stem cell culture; 2) Construct ES cell lines and media formulations for sale to the research community; 3) Produce a 'best practice' translatable manufacturing process; 4) To promote and market the technology to potential development partners and licensees.

沃德博士开发了一种使能技术并获得专利，该技术允许胚胎干细胞在高密度3D培养中生长，而不需要潜在有害的小鼠饲养细胞系和昂贵的生长因子。该技术有助于维持胚胎干细胞长时间（至少30天）处于未分化状态，并可以显着减少与当前方法相关的技术负担。通过提供一种简单，具有成本效益的小鼠和人类ES细胞大体积培养的途径，我们将有助于改变干细胞的效用，并扩大其作为研究工具的用途，并最终作为退行性疾病的治疗方法。本研究的目的是：1）优化和完善3D干细胞培养方法; 2）构建ES细胞系和培养基配方，销售给研究界; 3）产生“最佳实践”可翻译的制造工艺; 4）向潜在的开发合作伙伴和许可方推广和销售该技术。

项目成果

Novel peptides for deciphering structural and signalling functions of E-cadherin in mouse embryonic stem cells.

• DOI: 10.1038/srep41827
• 发表时间: 2017-02-07
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Segal JM;Ward CM
• 通讯作者: Ward CM

Sox2 is essential for formation of trophectoderm in the preimplantation embryo.

Sox2 对于植入前胚胎中滋养外胚层的形成至关重要。

• DOI: 10.1371/journal.pone.0013952
• 发表时间: 2010-11-12
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Keramari M;Razavi J;Ingman KA;Patsch C;Edenhofer F;Ward CM;Kimber SJ
• 通讯作者: Kimber SJ

Familial Alzheimer's disease modelling using induced pluripotent stem cell technology.

• DOI: 10.4252/wjsc.v6.i2.239
• 发表时间: 2014-04-26
• 期刊: World journal of stem cells
• 影响因子: 4.1
• 作者: Mohamet, Lisa;Miazga, Natalie J;Ward, Christopher M
• 通讯作者: Ward, Christopher M

Abrogation of E-cadherin-mediated cellular aggregation allows proliferation of pluripotent mouse embryonic stem cells in shake flask bioreactors.

• DOI: 10.1371/journal.pone.0012921
• 发表时间: 2010-09-23
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Mohamet L;Lea ML;Ward CM
• 通讯作者: Ward CM

Loss of function of e-cadherin in embryonic stem cells and the relevance to models of tumorigenesis.

• DOI: 10.1155/2011/352616
• 发表时间: 2011
• 期刊: Journal of oncology
• 作者: Mohamet L;Hawkins K;Ward CM
• 通讯作者: Ward CM